Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Evaluation of Antimicrobial, Antithrombin, and Antioxidant Activity of Dioscorea batatas Decne

마(Dioscorea batatas Decne)의 항균, 항산화 및 항혈전 활성 평가

  • Kim, Jee-In (Dept. of Biological Science, Andong National University) ;
  • Jang, Han-Su (Gyeongbuk Institute for Bioindustry) ;
  • Kim, Jong-Sik (Dept. of Biological Science, Andong National University) ;
  • Sohn, Ho-Yong (Dept. of Food and Nutrition, Andong National University)
  • Received : 2009.04.14
  • Accepted : 2009.06.05
  • Published : 2009.06.28
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Abstract

Yam (Dioscorea batatas Decne) has been recognized as healthy food due to its various biological activities, such as anti-obesity, anti-constipation, anti-proliferation, anti-mutagenic activity, and decrease of blood glucose and cholesterol level. In this study, the methanol extract and its organic solvent fractions were prepared from dried Dioscorea batatas Decne, and their antimicrobial, antithrombin, and antioxidant activity were evaluated, respectively. The 66.7% of methanol extract was fractioned into water residue, and most of total-polyphenol/total-flavonoids were found in ethylacetate fraction. This ethylacetate fraction at $500\;{\mu}g/disc$ concentration showed strong antibacterial activity against gram-negative and gram-positive bacteria, except Staphylococcus aureus. Antifungal activity was not observed in methanol extract and its fractions. But, strong antithrombin activity was found in methanol extract. At 4.8 mg/mL concentration, thrombin time was 99.5 sec, which is coincides with 30% activity of aspirin, a commercial available antithrombosis agent. The ethylacetate fraction showed strong DPPH scavenging activity ($IC_{50}$ of $80.5\;{\mu}g/mL$), compared to vitamin C ($IC_{50}$ of $15.2\;{\mu}g/mL$). Also, the ethylacetate fraction showed strong SOD-like activity and reducing power, which are coincide with 43% of vitamin C and 82.7% of butylated hydroxytoluene activity, respectively.

본 연구에서는 마의 부가적인 생리활성 탐색을 위해 건조 마로부터 메탄올 추출물을 조제하고, 이로부터 헥산, 에틸아세테이트, 부탄올의 다양한 유기용매 분획물 및 물 잔류물을 조제하여 각각의 항균, 항혈전 및 항산화 활성을 평가하였다. 마 추출물의 66.7%는 물 잔류물로서, 메탄올 추출물의 상당량이 수용성 성분임을 확인하였으며, 총 폴리페놀 및 총 플라보노이드 함량은 에틸아세테이트 분획에서 가장 높게 나타났다. 항균 활성의 경우, 에틸아세테이트 분획물 $500\;{\mu}g/disc$에서 Staphylococcus aureus를 제외한 실험세균 모두에서 우수한 항세균 활성이 나타났으며, 항진균 활성은 모든 시료에서 나타나지 않았다. 항혈전 활성은 메탄올 추출물에서 우수하였으며, 4.8 mg/mL 농도에서 99.5초의 트롬빈 타임을 나타내어 아스피린과 동일 활성을 위해서는 3.2배 농도가 필요하였다. 메탄올 추출물 및 유기용매 분획물의 항산화 활성 평가 결과, 에틸아세테이트 분획이 $80.5\;{\mu}g/mL$의 $IC_{50}$를 나타내어 우수한 DPPH 소거능을 나타내었으며, SOD 유사활성은 vitamin C의 43%, 환원력의 경우 BHT의 82,7%를 나타내어 매우 강력한 항산화 활성 물질을 포함하고 있음을 확인하였다.

Keywords

References

  1. Ahn J. H., K. H. Son, H. Y. Sohn, and S. T. Kwon. 2005. In vitro culture of adventitious roots from Dioscorea nipponica Makino for the production of steroidal saponins. Kor. J. Plant Biotechnol. 32: 317-223
  2. Han, Y. N., S. H. Hahn, and I. R. Lee. 1990. Purification of mucilages from Dioscorea batatas and D. japonica and their content analysis. Kor. J. Pharmacogn. 21: 274-283
  3. Hironaka, K., K. Takada, and K. Ishilbashi. 1990. Chemical composition of mucilage of chinese yam. Nippon Shokuhin Kogyo Gakkaishi 37: 48-51 https://doi.org/10.3136/nskkk1962.37.48
  4. Kang, T. H., S. Choi, T. Lee, M. Son, and S. Y. Kim. 2008, Characteristics of antidiabetic effect of Dioscorea rhizoma (1) - Hypoglycemic effect. Kor. J. Food Nutr. 21: 425-429
  5. Kang, T. H., S. Choi, T. Lee, M. Son, J. Park, and S. Y. Kim. 2008, Characteristics of antidiabetic effect of Dioscorea rhizoma (2) - prevention of diabetic neuropathy by NGF induction. Kor. J. Food Nutr. 21: 430-435
  6. Kim M. W. 2001. Effects of H2O-fraction of Dioscorea japonica Thunb and selenium on lipid peroxidation in streptozotocin-induced diabetic rats. Kor. J. Soc. Food Cookery Sci. 17, 344-352
  7. Kim, E. Y, I. H. Baik, J. H. Kim, S. R. .Kim, and M. R. Rhyu. 2004. Screening of the antioxidant activity of some medicinal plants. Kor. J. Food Sci. Technol. 36: 333-338
  8. Kim, J. O., M. J. Jung, H. J. Choi, J. T. Lee, A. K., Lim, J. H. Hong, and D. I. Kim. 2008. Antioxidant and biological activity of hot water and ethanol extracts from Phellinus linteus. J. Kor. Soc. Food Sci Nutr. 37: 684-690 https://doi.org/10.3746/jkfn.2008.37.6.684
  9. Kum, E. J., S. J. Park, B. H. Lee, J. S. Kim, K. H. Son, and H. Y. Sohn. 2006. Antifungal activity of phenanthrene derivatives from aerial bulbils of Dioscroea batatas decne. J. Life Sci. 16, 647-652 https://doi.org/10.5352/JLS.2006.16.4.647
  10. Kwon, C. S., H. Y. Sohn, S. H. Kim, J. H. Kim, K. H. Son, J. S. Lee, J. K. Lim, and J. S. Kim. 2003. Anti-obesity effect of Dioscorea nipponica Makino with lipase-inhibitory activity in rodents. Biosci. Biotechnol. Biochem. 67, 1451-1456 https://doi.org/10.1271/bbb.67.1451
  11. Kwon, C. S., I. S. Son, H. Y. Shim, I. S. Kwun, and K. M. Chung. 1999. Effects of yam on lowering cholesterol level and its mechanism. Kor. J. Food Nutr. 32: 637-643
  12. Kwon, E. G., E. M. Choe, and S. J. Gu. 2001. Effects of mucilage from yam (Dioscorea batatas DECNE) on blood glucose and lipid composition in alloxan-induced diabetic mice. Kor. J. Food Sci. Technol. 33, 795-801
  13. Lee I.-S., S.-Y. Chung, C.-S. Shim, and S. J. Koo. 1995. Inhibitory effects of yam (Dioscorea batatas DECNE) extracts on the mutagenicity. Kor. J. Soc. Food Sci.. 11: 351-355
  14. Lee, B. Y., and H. K. Kim. 1998. Quality properties of korean yam by various drying methods. Kor. J. Food Sci. Technol. 30: 877-882
  15. Marklund, S., and G. Marklund. 1974. Involvement of superoxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem. 47: 469-474 https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  16. Na, M., B. S. Min, R. B. An, W. Jin, Y. H. Kim, K. S. Song, Y. H. Seong and K. Bae. 2004. Effect of the rhizomes of Astilbe chinensis on UVB-induced inflammatory response. Phytother. Res. 18: 1000-1004 https://doi.org/10.1002/ptr.1599
  17. Park, C. S. 2005. Antioxidant and nitrite scavenging ability of medicinal plant extracts. Kor. J. Food Preserv. 12: 631-636
  18. Park, C. S., K. M. Yang, and M. L. Kim. 2006. Functional properties of medicinal plant extracts. Kor. J. Food. Cookery Sci. 23: 720-727
  19. Ryu, H. Y., E. J. Kum, K. H. Bae, Y. K. Kim, I. S. Kwon, and H. Y. Sohn. 2007. Evaluation for the antimicrobial, antioxidant and antithrombosis activity of Korean traditional liquors. Kor. J. Microbiol. Biotechnol. 35: 310-315
  20. Ryu, H. Y., S. J. Park, B. H. Lee, and H. Y. Sohn. 2007. Control of yam putrefactive psychrotrophic bacterium using clove oil and preparation of functional Fresh-cut. Kor. J. Microbiol. Biotechnol. 35: 66-72
  21. Ryu, H. Y., Y. S. Kim, S. J. Park, B. H. Lee, S. T. Kwon and H. Y. Sohn. 2006. Isolation and characterization of yamputrefactive psychrotrophic bacteria from rotted yam. Kor. J. Microbiol. Biotechnol. 34: 109-114
  22. Shin, K. O., J. R. Jeon, J. S. Lee, J. Y. Kim, C. H. Lee, S. D. Kim, Y. S. Yu, and D. H. Nam. 2006. Lactic acid fermentation of chinese yam (Dioscorea batatas Decne) flour and its pharmacological effect on gastrointestinal function in rat model. Biotechnol. Bioprocess Eng. 11: 240-244 https://doi.org/10.1007/BF02932037
  23. Shin, S. R. 2004. Changes on the components of yam snack by processing methods. Kor. J. Food Preservation 11, 516-521
  24. Sohn, H. Y. H. Y. Ryu, Y. Jang, H. S. Jang, Y. M. Park, and S. Y. Kim. 2008. Evaluation of antimicrobial, antithrombin, and antioxidant activity of aerial part of Saxifraga stolonifera. Kor. J. Microbiol. Biotechnol. 36: 195-200
  25. Sohn, H. Y., C. S. Kwon, K. H. Son, G. S. Kwon, H. Y. Ryu, and E. J. Kum. 2006. Antithrombin and thrombosis prevention activity of buckwheat seed, Fagopyrum esculentum Moench. J. Kor. Soc. Food Sci. Nutr. 35: 132-138 https://doi.org/10.3746/jkfn.2006.35.2.132
  26. Sohn, H. Y., K. H. Son, C. S. Kwon, G. S. Kwon, and S. S. Kang. 2004. Antimicrobial and cytotoxic activity of 18 prenylated flavonoids isolated from medicinal plants: Morus alba L., Morus mongolica Schneider, Broussnetia papyrifera (L.) Vent, Sophora flavescens Ait and Echinosophora koreensis Nakai. Phytomedicine 11: 666-672 https://doi.org/10.1016/j.phymed.2003.09.005