서울시내 수계시설에서 분리된 Legionella spp.의 병원성에 대한 분자역학적 연관성

Molecular Epidemiological Relationship of the Pathogenicity of Legionella spp. Isolated from Water Systems in Seoul

  • 김진아 (서울특별시 보건환경연구원 역학조사팀) ;
  • 정지헌 (서울특별시 보건환경연구원 역학조사팀) ;
  • 김수진 (서울특별시 보건환경연구원 역학조사팀) ;
  • 진영희 (서울특별시 보건환경연구원 역학조사팀) ;
  • 오영희 (서울특별시 보건환경연구원 역학조사팀) ;
  • 한기영 (서울특별시 보건환경연구원 역학조사팀)
  • Kim, Jin-Ah (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Jung, Ji-Hun (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kim, Soo-Jin (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Jin, Young-Hee (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Oh, Young-Hee (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Han, Gi-Young (Seoul Metropolitan Government Research Institute of Public Health and Environment)
  • 투고 : 2009.03.27
  • 심사 : 2009.04.28
  • 발행 : 2009.06.30

초록

레지오넬라균은 치명적인 폐렴을 일으킬 수 있는 균으로서, 기후 온난화와 함께 노출의 수위가 높아지고 있는 병원성균이다. 본 연구는 2008년 6월부터 8월까지 서울시내 25개구 소재 수계시설에서 분리한 레지오넬라균 73개 주에 대해 혈청형, Dot/Icm라 일컬어지는 병원성 유전자 분석 그리고 Pulsed field gel electrophoresis (PFGE)를 실시하여 유전자형과 그들 사이의 상관관계를 파악하였다. 73개주 중 69주는 Legionella pneumophila로 혈청형의 분포는 sg1 43주, sg6 9주, sg5 8주, sg3 8주, sg2 1주로 파악되었으며, Legionella spp. 4개주는 Legionella nautarum였다. 분리된 Legionella pneumophila 대부분이 여러 개의 병원성 유전자를 보유하고 있었으며, 반면에 Legionella nautarum은 병원성 유전자가 많이 결핍되어있었다. PFGE pattern을 분석해 볼 때, Legionella pneumophila가 동일한 혈청형안에서 다양하게 분화되어감을 볼 수 있었으며, 병원성 유전자의 분포와 깊은 상관관계를 보였다.

Legionella spp. is the causative agent of Legionellosis, which induces a potentially fatal form of pneumonia. With a concentrated performance during the summer of 2008, we secured 73 isolates from the water systems of 25 wards in Seoul. We analysed serotypes, pathogenic genes (Dot/Icm), and patterns of pulsed-field gel electrophoresis (PFGE) in an attempt to confirm relationships among them. Different from the previous year's pattern (2007), among the isolates, 69 were Legionella pneumophila and 4 were Legionella spp. The serotype distribution of Legionella pneumophila was sg1 43, sg6 9, sg5 8, sg3 8, and sg2 1. The serotype for the 4 Legionella spp. was Legionella nautarum. Most of the Legionella pneumophila had several pathogenic genes. On the other hand, the 4 Legionella spp. were defective in pathogenicity in genomic terms. The PFGE patterns of the serotypes showed a tendency for diversity of Legionella pneumophila and a close correlation with genetic pathogenicity.

키워드

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