Journal of Powder Materials (한국분말재료학회지)

The Korean Powder Metallurgy & Materials Institute (한국분말재료학회 (*구 분말야금학회))
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Response Characteristics of Electrochemical Non-enzyme Immunosensor using Fe3O4 Nanoparticle

Fe3O4 나노분말을 이용한 전기화학적 비효소 면역센서 응답특성

  • Kim, Chang-Kyu (Nuclear Materials Research Division, Korea Atomic Energy Research Institute (KAERI)) ;
  • Lee, Gyoung-Ja (Nuclear Materials Research Division, Korea Atomic Energy Research Institute (KAERI)) ;
  • Uhm, Young-Rang (Nuclear Materials Research Division, Korea Atomic Energy Research Institute (KAERI)) ;
  • Lee, Min-Ku (Nuclear Materials Research Division, Korea Atomic Energy Research Institute (KAERI)) ;
  • Rhee, Chang-Kyu (Nuclear Materials Research Division, Korea Atomic Energy Research Institute (KAERI))
  • 김창규 (한국원자력연구원 원자력재료연구부 나노소재응용랩) ;
  • 이경자 (한국원자력연구원 원자력재료연구부 나노소재응용랩) ;
  • 엄영랑 (한국원자력연구원 원자력재료연구부 나노소재응용랩) ;
  • 이민구 (한국원자력연구원 원자력재료연구부 나노소재응용랩) ;
  • 이창규 (한국원자력연구원 원자력재료연구부 나노소재응용랩)
  • Published : 2009.06.28
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Abstract

In this paper, the electrochemical non-enzyme immunosensor has been developed for the determination of salmonella antigen, using inverse voltammetry. For the estimation of salmonella antigen concentration, the $Fe_3O_4$ nanoparticles synthesized by microemulsion method were conjugated with salmonella antigen. Then, the immunocomplex between antibody immobilized on the transducer surface and antigen containing a magnetic nanoparticles was formed. From the linear relationship between the reduction peak current of Fe(III) and salmonella antigen concentration, it is suggested that the electrochemical non-enzyme biosensor is applicable to detect salmonella antigen in the concentration range of $10^1-10^5$ CFU/ml.

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