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http://dx.doi.org/10.4150/KPMI.2009.16.3.180

Response Characteristics of Electrochemical Non-enzyme Immunosensor using Fe3O4 Nanoparticle  

Kim, Chang-Kyu (Nuclear Materials Research Division, Korea Atomic Energy Research Institute (KAERI))
Lee, Gyoung-Ja (Nuclear Materials Research Division, Korea Atomic Energy Research Institute (KAERI))
Uhm, Young-Rang (Nuclear Materials Research Division, Korea Atomic Energy Research Institute (KAERI))
Lee, Min-Ku (Nuclear Materials Research Division, Korea Atomic Energy Research Institute (KAERI))
Rhee, Chang-Kyu (Nuclear Materials Research Division, Korea Atomic Energy Research Institute (KAERI))
Publication Information
Journal of Powder Materials / v.16, no.3, 2009 , pp. 180-184 More about this Journal
Abstract
In this paper, the electrochemical non-enzyme immunosensor has been developed for the determination of salmonella antigen, using inverse voltammetry. For the estimation of salmonella antigen concentration, the $Fe_3O_4$ nanoparticles synthesized by microemulsion method were conjugated with salmonella antigen. Then, the immunocomplex between antibody immobilized on the transducer surface and antigen containing a magnetic nanoparticles was formed. From the linear relationship between the reduction peak current of Fe(III) and salmonella antigen concentration, it is suggested that the electrochemical non-enzyme biosensor is applicable to detect salmonella antigen in the concentration range of $10^1-10^5$ CFU/ml.
Keywords
$Fe_3O_4$; Biosensor; Non-enzyme; Electrochemical; Inverse voltammetry;
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