한국광학회지 (Korean Journal of Optics and Photonics)

  • 제20권3호
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  • Pages.182-188
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  • 2009
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  • 1225-6285(pISSN)
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  • 2287-321X(eISSN)
한국광학회 (Optical Society of Korea)
권호 표지
DOI QR코드

DOI QR Code

공기주입 장치와 공기제거 장치를 사용한 표면 플라즈몬 공명 타원계측기

Surface Plasmon Resonance Ellipsometry Using an Air Injection System with an Extraction of Air System

  • 이홍원 (한남대학교 광.전자물리학과) ;
  • 조은경 (한남대학교 광.전자물리학과) ;
  • 조재흥 (한남대학교 광.전자물리학과) ;
  • 원종명 (안동대학교 물리학과) ;
  • 신기량 (안동대학교 물리학과) ;
  • 제갈원 (한국표준과학연구원 나노바이오융합연구단) ;
  • 조용재 (한국표준과학연구원 나노바이오융합연구단) ;
  • 조현모 (한국표준과학연구원 나노바이오융합연구단)
  • Lee, Hong-Won (Dept. of Applied Optics and Electromagnetics, Hannam University) ;
  • Cho, Eun-Kyoung (Dept. of Applied Optics and Electromagnetics, Hannam University) ;
  • Jo, Jae-Heung (Dept. of Applied Optics and Electromagnetics, Hannam University) ;
  • Won, Jong-Myoung (Dept. of Physics, Andong National University) ;
  • Shin, Gi-Ryang (Dept. of Physics, Andong National University) ;
  • CheGal, Won (Center for Nano-Bio Convergence Research, KRISS) ;
  • Cho, Yong-Jai (Center for Nano-Bio Convergence Research, KRISS) ;
  • Cho, Hyun-Mo (Center for Nano-Bio Convergence Research, KRISS)
  • 발행 : 2009.06.25
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초록

완충용액과 분석시료의 확산으로 발생하는 측정 오차를 최소화하기 위하여 공기주입 장치와 공기제거 장치를 설치한 표면 플라즈 몬 공명 타원계측기(surface plasmon resonance ellipsometer: SPRE)를 새로이 제안하고 이를 제작하였다. SPRE에서 완충용액과 분석시료간의 상호 확산은 분석시료의 농도를 변화시켜 굴절률 측정에 영향을 주고, 이 결과 생체분자 물질의 접합 특성이 명확하게 나타나지 않는다. 이러한 SPRE의 측정 장치에 공기를 주입하는 장치를 추가로 설치하여 두 용액의 확산을 막고, 특히 불필요한 공기가 채널 내부로 유입되어 생기는 잡음신호를 없애기 위하여 공기제거 장치를 사용함으로써 신뢰성 있는 SPRE의 측정 결과를 얻을 수 있음을 확인하였다.

The surface plasmon resonance ellipsometer (SPRE), using a multiple air injection system with an extraction of air system, has been proposed and developed to minimize measurement error of signals due to diffusion of reagent into running buffer. Since the diffusion of reagent into running buffer affects the refractive index of the running buffer by changing the concentration, characteristics of binding between various bio-molecules don't appear clearly in measurement results. The diffusion between running buffer and reagent can be blocked by using an air bubble injection system. An extraction of air system is used to remove the noise signal due to unnecessary air bubbles flowing in a channel. Reliability of measurement results has been improved by using the valve system.

키워드

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