Food Science and Biotechnology

  • 제18권2호
  • /
  • Pages.475-480
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  • 2009
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  • 1226-7708(pISSN)
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  • 2092-6456(eISSN)
한국식품과학회 (Korean Society of Food Science and Technology)
권호 표지

Effects of Aucubin Isolated from Eucommia ulmoides on UVB-induced Oxidative Stress in Human Keratinocytes HaCaT

  • Ho, Jin-Nyoung (Department of Food and Biotechnology, Han-Seo University) ;
  • Cho, Hong-Yon (Department of Food and Biotechnology, Korea University) ;
  • Lim, Eun-Jeong (Department of Food and Nutrition, Hanyang Women's College) ;
  • Kim, Hye-Kyung (Department of Food and Biotechnology, Han-Seo University)
  • 발행 : 2009.04.30
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초록

Ultraviolet B (UVB) radiation provokes the generation of reactive oxygen species (ROS) in the cells and skin, which induce oxidative stress in the exposed cells, leading to photoaging and cancer. Using the human keratinocytes HaCaT cell line, we investigated the photoprotective effects of aucubin isolated from Eucommia ulmoides. Pretreatment with aucubin markedly suppressed UVB-induced oxidative stress, which manifests as a decrease in intracellular lipid peroxidation, elevation of catalase activity, and reduced glutathione content. In addition, aucubin significantly reduced expression of matrix metalloproteinase-1 (MMP-1) protein (54%) and mRNA. Taken together, these results suggest that aucubin may offer protection against UVB-induced oxidative stress and may be used as a potential agent in prevention of UVB-induced photoaging.

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