Macromolecular Research

The Polymer Society of Korea (한국고분자학회)
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Nanopatterning of Proteins Using Composite Nanomold and Self-Assembled Polyelectrolyte Multilayers

  • Kim, Sung-Kyu (School of Chemical and Biological Engineering, Seoul National University) ;
  • Kim, Byung-Gee (School of Chemical and Biological Engineering, Seoul National University) ;
  • Lee, Ji-Hye (Department of Chemical Engineering, Chungnam National University) ;
  • Lee, Chang-Soo (Department of Chemical Engineering, Chungnam National University)
  • Published : 2009.04.25
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Abstract

This paper describes the simple nanopatterning of proteins on polyelectrolyte surfaces using microcontact printing with a nanopatternable, hydrophilic composite nanomold. The composite nanomold was easily fabricated by blending two UV-curable materials composed of Norland Optical Adhesives(NOA) 63 and poly(ethylene glycol) dimethacrylate(PEG-DMA). NOA 63 provided stable nanostructure formation and PEG-DMA induced high wettability of proteins in the nanomold. Using the composite mold and functionalized surface with polyelectrolytes, the fluorescent, isothiocyanate-tagged, bovine serum albumin(FITC-BSA) was successfully patterned with 8 nm height and 500 nm width. To confirm the feasibility of the protein assay on a nanoscale, a glycoprotein-lectin assay was successfully demonstrated as a model system. As expected, the lectins correctly recognized the nano-patterned glycoproteins such as chicken ovalbumin. The simple preparation of composite nanomold and functionalized surface with a universal platform can be applied to various biomolecules such as DNA, proteins, carbohydrates, and other biomolecules on a nanoscale.

Keywords

References

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