Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effects of Rubus Coreanus Miquel on the Apoptosis of U937 Cells

복분자가 U937세포의 apoptosis에 미치는 효과

  • Published : 2009.12.25
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Abstract

Methanol extracts of Rubus Coreanus Miquel (RCM) were found to exhibit apoptosis induction of U937 human histiocytic leukemia cells. Treatment of RCM exerted strong cytotoxicity against U937 human leukemia cells. RCM induced apoptosis of U937 leukemia cells in a dose dependent manner. Nitric oxide (NO) production was increased in RCM-treated RAW264.7 macrophage cell line. RCM increased the p53 and $NF{\kappa}B$ gene and decreased the $I{\kappa}B$ gene expression in U937 cells. RCM also increased the $NF{\kappa}B$ protein expression, but decreased the PCNA and BcL-xL protein expression in cultured U937 cells. These data suggest that RCM are effective on the apoptosis induction of human leukemia cells and anti-cancer properties.

Keywords

References

  1. Cha, H.S., Lee, M.K., Hwang, J.B., Park, K.M. Physicochemical characteristics of Rubus coreanus Miquel. J. Korean Soc. Food Sci. Nutr., 30(6):1021-1025, 2001
  2. 윤 인, 위지향, 문제학, 안태희, 박근형. 복분자열매에서 항산화활성을 지닌 quercetin의 분리 및 동정. 한국식품과학회지 35(3):499-502, 2003
  3. 최 면, 신건재, 최근표, 도재호, 김종대. 흰쥐의 항산화활성에 미치는 홍삼, 삼백초, 복분자 추출물의 상승효과. 한국약용작물학회지 11(2):148-154, 2003
  4. 원경숙, 이태원, 은재순, 송정모. 복분자가 생쥐의 특이적 면역 반응에 미치는 영향. 동의생리병리학회지 17(3):656-661, 2003
  5. 이택렬, 김대근, 소준노, 권 진, 송정모, 은재순, 복분자 미숙과 및 성숙과가 생쥐의 복강 Macrophages에 미치는 영향. 대한동의병리학회지 17(4):991-995, 2003
  6. Guang-zhao Li, 채옥희, 송창호. 생체실험에서 복분자의 compound 48/80에 의한 아나필락틱 쇽과 혈관투과성 증가의 억제효과. 대한체질인류학회지 17(2):99-107, 2004
  7. Mosmann, T. Rapid colorimetric assay for cellular growth and survival application to proliferation and cytotoxic assays. J. Immunol. Methods, 65: 55-63, 1983 https://doi.org/10.1016/0022-1759(83)90303-4
  8. Telford, W.G., King, L.E. and Fraker, P.J. Evaluation of glucocorticoid-induced DNA fragmentation in mouse thymocytes by flow cytometry. Cell Prolif., 24: 447-459, 1991 https://doi.org/10.1111/j.1365-2184.1991.tb01173.x
  9. Nicoletti, I., Migliorati, G., Pagliacci, M.C., Grignani, G. and Riccardi, C.A. Rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J. Immunol. Methods, 139: 271-279, 1991 https://doi.org/10.1016/0022-1759(91)90198-O
  10. Kondo, Y. and Takano, F. Nitric oxide production in mouse peritoneal macrophage enhanced with glycyrrhizin. Biol. Pharm. Bull. 17(5):759-761, 1994 https://doi.org/10.1248/bpb.17.759
  11. Miyashita, T. and Reed, J.C., Tumor suppressor p53 is a direct transcriptional activator of the human bax gene. Cell, 80: 293-299, 1995 https://doi.org/10.1016/0092-8674(95)90412-3
  12. W. Neil Burnette. 'Western blotting': electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A'. Analytical Biochemistry, 112(2):195-203, 1981 https://doi.org/10.1016/0003-2697(81)90281-5
  13. Dowdy, S. and Wearden, S. Statistics for research, Wiley, New York, p 262, 1983
  14. Thomsen, L.L., Ching, L.M. and Baguley, B.C. Evidence for the production of nitric oxide by activated macrophages treated with the antitumor agents flavone-8-acetic acid and xantherone-4-acetic acid. Cancer Res., 51: 6073-6078, 1991
  15. Liew, F.Y., Li, Y., Severn, A., Millott, S.M., Schmidt Salter, M. and Moncada, S. A possible novel pathway of regulation by murine T helper type-2(Th2) cells of a Th1 cell activity via the modulation of the induction of nitric oxide synthetase on macrophages. Eur. J. Immunol. 21: 2489-2494, 1991 https://doi.org/10.1002/eji.1830211027
  16. Kastan, M.B., Canman, C.E. and Leonard, C.J. p53, cell cycle control and apoptosis: implications for cancer. Cancer Metas. Rev. 14(1):3-15, 1995 https://doi.org/10.1007/BF00690207
  17. Floryk, D. and Huberman, E. Differentiation of androgen-independent prostate cancer PC-3 cells is associated with increased NF-κB activity, Cancer Res. 65(24):11588-11596, 2005 https://doi.org/10.1158/0008-5472.CAN-05-1831
  18. Dai, Y., Rahmani, M. and Grant, S. An intact NF-$\kappa$B pathway is required for histone deacetylase inhibitor-induced G1 arrest and maturation in U937 human myeloid leukemia cells, Cell Cycle, 2: 467-472, 2003
  19. Barnes, P.J., Karin, M. Nuclear Factor-$\kappa$B-a pivotal transcription factor in chronic inflammatory diseases. N Engl. J Med., 336: 1066-1071, 1997 https://doi.org/10.1056/NEJM199704103361506
  20. Jacobs, M.D., Harrison, S.C. Structure of an $I{\kappa}B{\alpha}/NF-{\kappa}B $ Complex. Cell 95(6):749-758, 1998 https://doi.org/10.1016/S0092-8674(00)81698-0
  21. Scott, M., Bonnefin, P., Vieyra,, D., Boisvert, F.M., Young, D., Bazett-Jones, D.P. and　Riabowl, K. UV-induced binding of ING1 to PCNA regulates the induction of apoptosis, Journal of Cell Science, 114: 3455-3462, 2001