동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제23권6호
  • /
  • Pages.1392-1398
  • /
  • 2009
  • /
  • 1738-7698(pISSN)
  • /
  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

괴화(槐花) 물 추출물의 항염증 효과

Anti-inflammatory Effects of Sophora Japonica Aqueous Extract

  • 배기상 (원광대학교 한의과대학 본초학교실) ;
  • 조범연 (원광대학교 한의과대학 본초학교실) ;
  • 김민선 (원광대학교 한의과대학 본초학교실) ;
  • 박경철 (원광대학교 한의과대학 본초학교실) ;
  • 구본순 (원광대학교 한의과대학 본초학교실) ;
  • 서상완 (충북 제천 전통의약 산업센터) ;
  • 김성규 (충북 제천 전통의약 산업센터) ;
  • 윤승원 (충북 제천 전통의약 산업센터) ;
  • 정원석 (전남 한방 산업 진흥원) ;
  • 함경완 (원광대학교 한의과대학 본초학교실) ;
  • 송호준 (원광대학교 한의과대학 본초학교실) ;
  • 윤명자 (원광대학교 의과대학 전정와우 연구센터) ;
  • 전호성 (원광대학교 한의학전문대학원 제3의학과) ;
  • 권강범 (원광대학교 한의과대학 한방생리학교실) ;
  • 김재효 (원광대학교 한의과대학 경혈학교실) ;
  • 박성주 (원광대학교 한의과대학 본초학교실)
  • Bae, Gi-Sang (Department of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Jo, Beom-Yeon (Department of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Kim, Min-Sun (Department of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Park, Kyoung-Chel (Department of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Koo, Bon-Soon (Department of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Seo, Sang-Wan (ChungBuk Oriental Medicine Center) ;
  • Kim, Sung-Gyu (ChungBuk Oriental Medicine Center) ;
  • Yun, Seung-Won (ChungBuk Oriental Medicine Center) ;
  • Jung, Won-Seok (Division of Constitutional Medicine Research, Korea Institute of Oriental Medicine) ;
  • Ham, Kyung-Wan (Department of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Song, Ho-Joon (Department of Herbology, College of Oriental Medicine, Wonkwang University) ;
  • Youn, Myung-Ja (Vestibulocochlear Research Center, College of Medicine, Wonkwang University) ;
  • Jeon, Ho-Seong (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Kwon, Kang-Beom (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Kim, Jae-Hyo (Department of Meridian & Acupoint, College of Oriental Medicine, Wonkwang University) ;
  • Park, Sung-Joo (Department of Herbology, College of Oriental Medicine, Wonkwang University)
  • 발행 : 2009.12.25
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The purpose of this study was to investigate the anti-inflammatory effects of aqueous extract from Sophora Japonica (SJ) on the RAW 264.7 cells. To evaluate the anti-inflammatory effects of SJ, we examined the cytokine productions including nitric oxide (NO), interleukin (IL)-1b, IL-6 and tumor necrosis factor-a (TNF-a) in lipopolysaccharide (LPS)-induced RAW 264.7 cells and also inhibitory mechanisms such as mitogen-activated protein kinases (MAPKs) and nuclear factor kappa b (NF-kB) using Western blot. SJ inhibited LPS-induced production of NO, TNF-a but not of IL-1b and IL-6 in RAW 264.7 cells. SJ inhibited the activation of MAPKs such as extracelluar signal-regulated kinase (ERK 1/2), c-Jun NH2-terminal kinase (JNK) and p38 but not of NF-kB in the LPS-stimulated RAW 264.7 cells. In conclusion, SJ down-regulated LPS-induced NO and TNF-a productions via MAPKs, which could be a clinical basis for inflammatory diseases and autoimmune diseases.

키워드

참고문헌

  1. 신민교. 임상본초학, 서울, 영림사, p 489, 2002
  2. Kite, G.C., Veitch, N.C., Boalch, M.E., Lewis, G.P., Leon, C.J., Simmonds, M.S. Flavonol tetraglycosides from fruits of Styphnolobium japonicum (Leguminosae) and the authentication of Fructus Sophorae and Flos Sophorae. Phytochemistry 70(6):785-794, 2009 https://doi.org/10.1016/j.phytochem.2009.04.003
  3. Kim, J.M., Yun Choi, H.S.　Anti-platelet effects of flavonoids and flavonoid-glycosides from Sophora japonica.　Arch Pharm Res. 31(7):886-890, 2008 https://doi.org/10.1007/s12272-001-1242-1
  4. Tang, Y.P., Zhu, H.X., Duan, J.A. Two new isoflavone triglycosides from the small branches of Sophora japonic a。J Asian Nat Prod Res. 10(1-2):65-70, 2008 https://doi.org/10.1080/10286020701273858
  5. Tang, Y., Yang, R., Duan, J.A., Shang, E., Su, S., Zhu, M., Qian, D.　Isoflavone tetraglycosides from Sophora japonica Leaves.　J Nat Prod. 71(3):448-450, 2008 https://doi.org/10.1021/np070396t
  6. Jung, C.H., Zhou, S., Ding, G.X., Kim, J.H., Hong, M.H., Shin, Y.C., Kim, G.J., Ko, S.G.　Antihyperglycemic activity of herb extracts on streptozotocin-induced diabetic rats. Biosci Biotechnol Biochem. 70(10):2556-2559, 2006 https://doi.org/10.1271/bbb.60238
  7. Kim, B.H., Chung, E.Y., Min, B.K., Lee, S.H., Kim, M.K., Min, K.R., Kim, Y.　Anti-inflammatory action of legume isoflavonoid sophoricoside through inhibition on cyclooxygenase-2 activity. Planta Med. 69(5):474-476, 2003 https://doi.org/10.1055/s-2003-39712
  8. Chung, T.W., Moon, S.K., Chang, Y.C., Ko, J.H., Lee, Y.C., Cho, G., et al. Novel and therapeutic effect of caffeic acid and caffeic acid phenyl ester on hepatocarcinoma cells: complete regression of hepatoma growth and metastasis by dual mechanism. FASEB J. 18: 670-681, 2004
  9. Lee, T.K., Kim, D.I., Han, J.Y., Kim, C.H. Inhibitory effects of Scutellaria barbata D. Don. and Euonymus alatus Sieb. on aromatase activity of human leiomyomal cells. Immunopharm Immunotoxicol. 26: 315-327, 2004 https://doi.org/10.1081/IPH-200026840
  10. Kwqamata, H., Ochiai, H., Mantani, N., terasawa, K. Enhanced expression of inducible nitric oxide synthase by Juzen-taiho-to in LPS-activated RAW264.7 cells, a murine macrophage cell line. Am J Chin Med. 28: 217-226, 2000 https://doi.org/10.1142/S0192415X0000026X
  11. Lee, B.G., Kim, S.H. Zee, O.P., Lee, K.R., Lee, H.Y., Han, J.W., Lee, H.W. Suppression of inducible nitric oxide synthase expression in RAW 264.7 macrophages by two-carboline alkaloids extracted from Melia azedarach. Eur J Pharmacol. 406: 301-309, 2000 https://doi.org/10.1016/S0014-2999(00)00680-4
  12. Seo, W.G., Pae, H.O., Oh, G.S., Chai, K.Y., Yun, Y.G., Kwon, T.O., Chung, H.T. Inhibitory effect of ethyl acetate fraction from Cudrania tricuspidata on the expression of nitric oxide synthase gene in RAW 264.7 macrophages stimulated with interferon-and lipopolysaccharide. Gen Pharmacol. 35: 21-28, 2000 https://doi.org/10.1016/S0306-3623(01)00086-6
  13. Chiou, W.F., Chou, C.J., Chen, C.F. Camptothecin suppresses nitric oxide biosynthesis in RAW 264.7 macrophages. Life Sci. 69: 625-635, 2001 https://doi.org/10.1016/S0024-3205(01)01154-7
  14. Seo, W.G., Pae, H.O., Oh, G.S., Kim, N.Y., Kwon, T.O., Shin, M.K., Chai, K.Y., Chung, H.T. The aqueous extract of Rhodiola sachalinensis root enhances the expression of inducible nitric oxide synthase gene in RAW264.7 macrophage. J Ethnophamacol. 76: 119-123, 2001 https://doi.org/10.1016/S0378-8741(01)00220-3
  15. Horwood, N.J., Page, T.H., McDaid, J.P., Palmer, C.D., Campbell, J., Mahon, T., Brennan, F.M., Webster, D., Foxwell, B.M. Bruton's tyrosine kinase is required for TLR2 and TLR4-induced TNF, but not IL-6, production. J Immunol. 176(6):635-641, 2006
  16. Hirohashi, N., Morrison, D.C. Low-dose lipopolysaccharide (LPS) pretreatment of mouse macrophage modulates LPS-dependent interleukin-6 production in vitro. Infect Immun. 64(3):1011, 1996
  17. Matsuda, H., Morikawa, T., Ando, S., Toguchida, I. and Yoshikawa. M. Structural requirements of flavonoids for nitric oxide production inhibitory activity and mechanism of action. Bioorganic Med. Chem. 11: 1995-2000, 2003 https://doi.org/10.1016/S0968-0896(03)00067-1
  18. Calixto, J.B., Campos, M.M., Otuki, M.F., Santos, A.R. Anti-inflammatory compounds of plant origin. Part II. modulation of pro-inflammatory cytokines, chemokines and adhesion molecules. Planta Med. 70(2):93-103, 2004 https://doi.org/10.1055/s-2004-815483
  19. Tang, Y.P., Li, Y.F., Hu, J., Lou, F.C. Isolation and identification of antioxidants from Sophora japonica. J Asian Nat Prod Res. 4(2):123-128, 2002 https://doi.org/10.1080/10286020290027407
  20. Tang, Y., Lou, F., Wang, J., Zhuang, S. Four new isoflavone triglycosides from Sophora japonica. J Nat Prod. 64(8):1107-1110, 2001 https://doi.org/10.1021/np010081s
  21. Paniwnyk, L., Beaufoy, E., Lorimer, J.P., Mason, T.J. The extraction of rutin from flower buds of Sophora japonica. Ultrason Sonochem. 8(3):299-301, 2001 https://doi.org/10.1016/S1350-4177(00)00075-4
  22. Kang, S.C., Lee, C.M., Choi, H., Lee, J.H., Oh, J.S., Kwak, J.H., Zee, O.P. Evaluation of oriental medicinal herbs for estrogenic and antiproliferative activities. Phytother Res. 20(11):1017-1019, 2006 https://doi.org/10.1002/ptr.1987
  23. Mazal, P.R., Schaufler, R., Altenhuber Müller, R., Haitel, A., Watschinger, B., Kratzik, C., Krupitza, G., Regele, H., Meisl, F.T., Zechner, O., Kerjaschki, D., Susani, M. Derivation of nephrogenic adenomas from renal tubular cells in kidney-transplant recipients. N Engl J Med. 347(9):653-659, 2002 https://doi.org/10.1056/NEJMoa013413
  24. Abe, M., Suzuki, O., Tasaki, K., Tominaga, K., Wakasa, H. Analysis of lectin binding properties on human Burkitt's lymphoma cell lines that show high spontaneous metastasis to distant organs in SCID mice: the binding sites for soybean agglutinin lectin masked by sialylation are closely associated with metastatic lymphoma cells. Pathol Int. 46(12):977-983, 1996 https://doi.org/10.1111/j.1440-1827.1996.tb03577.x
  25. Matsumura, K., Nakasu, S., Nioka, H., Handa, J. Lectin histochemistry of normal and neoplastic peripheral nerve sheath. 1. Lectin binding pattern of normal peripheral nerve in man. Acta Neuropathol. 86(6):554-558, 1993 https://doi.org/10.1007/BF00294292
  26. la Sala, A., Gadina, M., Kelsall, B.L. G(i)-protein-dependent inhibition of IL-12 production is mediated by activation of the phosphatidylinositol 3-kinase-protein 3 kinase B/Akt pathway and JNK. J. Immunol. 175: 2994-2999, 2005 https://doi.org/10.4049/jimmunol.175.5.2994
  27. Wu, G.H., Zhang, Y.W., Wu, Z.H. Modulation of postoperative immune and inflammatory response by immune-enhancing enteral diet in gastrointestinal cancer patients. World J Gastroenterol. 7: 357-362, 2001 https://doi.org/10.3748/wjg.v7.i3.357
  28. Bryan, N.S. Nitrite in nitric oxide biology: cause or consequence? A systems based review. Free Radic Biol Med. 41: 691-701, 2006 https://doi.org/10.1016/j.freeradbiomed.2006.05.019
  29. Bhattacharyya, A., Pathak, S., Datta, S., Chattopadhyay, S., Basu, J., Kundu, M. Mitogen-activated protein kinases and NF-kappaB regulate H. pylori-mediated IL-8 release from macrophages. Biochem J. 366: 376-382, 2002
  30. Binetruy, B., Smeal, T., Kariu, M. Ha-Ras augments c-Jun activity and stimulates phosphoylation of its activation domain. Nature. 351: 122-127, 1991 https://doi.org/10.1038/351122a0
  31. Tracey, K.J., Cerami, A., Tumor necrosis factor : a pleiotropic cytokine and therapetic target. Annual Reviews of Medicine 45, 491-503, 1994 https://doi.org/10.1146/annurev.med.45.1.491
  32. Tracey, K.J., Beutler B., Lowry, S.F., Merryweather, J., Wolper, S., Milsark, I.W., Hariri, R.J., Fahey, J.J., Zentella, A., Albert, J.D., Shock and tissue injury induced by recombinant human cachectin. Science 232, 977-980, 1986 https://doi.org/10.1126/science.3754653
  33. Garrington, T.P., Johnson, G.L. Organization and regulation of mitogen-activated protein kinase signaling pathways. Curr Opin Cell Biol. 11: 211-218, 1999 https://doi.org/10.1016/S0955-0674(99)80028-3
  34. Seo, J.H., Lim, J.W., Kim, H., Kim, K.H. Helicobacter pylori in a Korean isolate activates mitogen-activated protein kinases. AP-1, and NF-kappaB and induces chemokine expression in gastric epithelial AGS cells. lab Invest. 84: 49-62, 2004 https://doi.org/10.1038/labinvest.3700010
  35. Lee, A.K., Sung, S.H., Kim, Y.C., Kim, S.G. Inhibition of lipopolysaccharide-inducible nitiric oxide synthase.TNF-a and COX-2 expression by suchinone effects on I-κBa phosphorylation. C/EBP and AP-1 activation. British J.Phamacol. 139: 11-20, 2003 https://doi.org/10.1038/sj.bjp.0705231
  36. Meng, F., Lowell, C.A. Lipopolysaccharide(LPS)-induced macrophage activation and signal transduction in the absence of Src-family kinase Hck, Fgr, and Lyn. J Exp Med. 185(9):1661, 1997 https://doi.org/10.1084/jem.185.9.1661
  37. Aggarwal, B.B. Signaling pathways of the TNF superfamily : a double-edged sword. Nature Reviews Immunology 3: 745-756, 2003 https://doi.org/10.1038/nri1184