한국식품과학회지 (Korean Journal of Food Science and Technology)

한국식품과학회 (Korean Society of Food Science and Technology)
권호 표지

방사선 조사된 라면수프의 조사선원에 따른 전자스핀공명 분석특성

Analytical Characteristics of Electron Spin Resonance for Identifying Irradiated Ramen Soup with Radiation Sources

  • 안재준 (경북대학교 식품공학과) ;
  • 이주운 (한국원자력연구원 정읍방사선과학연구소) ;
  • 정형욱 (식품의약품안전청) ;
  • 권중호 (경북대학교 식품공학과)
  • Ahn, Jae-Jun (Department of Food Science & Technology, Kyungpook National University) ;
  • Lee, Ju-Woon (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Chung, Hyung-Wook (Korea Food & Drug Administration) ;
  • Kwon, Joong-Ho (Department of Food Science & Technology, Kyungpook National University)
  • 발행 : 2009.04.30
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초록

시판 분말 라면수프(RS-1, RS-2)에 대하여 방사선 조사선원(감마선, 전자선, 0-20 kGy)에 따른 ESR 분석특성을 연구한 결과, 수프에 함유된 결정형 당이 방사선 에너지에 의해 free radical을 생성하는 것으로 나타났다. 즉, 방사선 처리되지 않은 2종의 라면수프에서는 $Mn^{2+}$ radical이 나타났으나, 조사시료의 ESR spectra는 RS-1에서 g = 2.011, 2.006, 2.002 및 1.999, RS-2에서 g = 2.010, 2.006, 2.002 및 1.999의 4개의 peak를 가진 multi-component signal이 각각 확인되어 조사여부의 판별이 가능하였다. 또한 감마선 및 전자선과 조사선량에 따른 ESR intensity를 비교한 결과, 동일 측정조건에서는 모든 선량에서 전자선 조사시료가 감마선 조사시료보다 높은 강도를 나타내었다. 라면수프의 방사선 조사선량과 ESR intensity 간의 R2는 0.9665 이상을 보이면서 각 signal의 intensity는 조사선량이 증가함에 따라 일정한 비율로 증가하였으며, g-value가 나타나는 자장영역은 일정하였다. 이들 중 강도가 큰 $g_2$(2.010-2.011)와 $g_3$(2.002)의 signal 증가는 조사선량과 높은 선형적 상관을 나타내었다($R^2$= 0.9750-0.9981).

The identification characteristics of irradiated commercial Ramen soup were investigated depending on radiation sources and doses by electron spin resonance (ESR) spectroscopy. Two commercial powder soups (RS-1, RS-2) were irradiated at 0 to 20 kGy under ambient conditions by both a Co-60 gamma irradiator and an electron beam (EB) accelerator, respectively. Crystalline sugar-induced multi-component signals with g-values of 2.010/2.011, 2.006, 2.002 and 1.999 were detected in the irradiated Ramen soup (RS-1, RS-2), whereas $Mn^{2+}$ signals were observed in non-irradiated samples, thereby distinguishing each other. Under the same analytical conditions, the intensity of ESR signals was higher in EB-irradiated samples than the gamma-irradiated ones. Determination coefficients ($R^2$) between irradiation doses and corresponding ESR responses were above 0.9665 in all the samples, and the magnetic field of specified g-value remained constant. The predominant ESR signals of $g_2$ (2.010-2.011) and $g_3$ (2.002) increased with corresponding doses of irradiation ($R^2$= 0.9750-0.9981).

키워드

참고문헌

  1. KATI. Korea Agro-Fisheries Trade Corporation homepage. http://www.at.or.kr. Accessed July 5, 2008
  2. Diel JF. Potential and actual applications of irradiated foods. pp.217-253. In: safety of Irradiated Foods, Marcel Dekker, Inc., New York, NY, USA (1990)
  3. KFDA. Korea Food Standard Code, 2-1-9~2-1-10 Korea Food & Drug Administration, Seoul, Korea (2008)
  4. WHO/FAO. Food irradiation - A technique for preserving and improving the safety of food. World Health Organization, Geneva, Switzerland (1988)
  5. IAEA. International Atomic Energy Agency homepage. http://nucleus.iaea.org/NUCLEUS/nucleus/Content/index.jsp. Accessed July 5, 2008
  6. Morehouse JM, Ku Y, Albrecht HL, Yang GC. Chromatographic and electron spin resonance investigations of gamma-irradiated frog legs. Radiat. Phys. Chem. 32: 61-68 (1991)
  7. Lesgards G, Raffi J, Pouliquen I, Chaouc AA, Giamarchi P, Prost M. Use of radiation-induced alkanes and alkenes to detect irradiated food containing lipids. J. AOAC Int. 70: 179-185 (1993)
  8. Schreiber GA, Schulzku G, Helle N, Bgl KW. Methods for routine control of irradiated food: optimization of a method for detection of radiation-induced hydrocarbons and its application to various foods. Radiat. Phys. Chem. 43: 433-444 (1994) https://doi.org/10.1016/0969-806X(94)90059-0
  9. Desrosiers MF, Simic MG. Postirradiation dosimetry of meat by electron spin resonance spectroscopy of bones. J. Agr. Food Chem.36: 601-603 (1988) https://doi.org/10.1021/jf00081a049
  10. Sanderson DCW, Slater C, Caims KJ. Detection of irradiated food. Nature 30: 23-24 (1989)
  11. Heide L, Bgl KW. The identification of irradiated spices with thermo- and chemiluminescence measurements. Int. J. Food Sci.Technol. 22: 93-103 (1987) https://doi.org/10.1111/j.1365-2621.1987.tb00463.x
  12. Schreiber GA, Ziegelmann B, Quitzsch G, Helle N, Bgl KW. Luminescence techniques to identify the treatment of foods by ionizing irradiation. Food Struct. 12: 385-396 (1993)
  13. Schreiber GA, Helle N, Bgl KW. An interlaboratory trial on the identification of irradiated spices, herbs, and spice-herb mixtures by thermoluminescence analysis. J. AOAC Int. 78: 88-93 (1995)
  14. Schreiber GA, Hoffmann A, Helle N, Bgl KW. Methods for routine control of irradiated food : Determination of the irradiation status of shellfish by thermoluminescence analysis. Radiat. Phys.Chem. 43: 533-544 (1994) https://doi.org/10.1016/0969-806X(94)90164-3
  15. CEN. Detection of irradiated food irradiated food containing cellulose,method by ESR spectroscopy. European Committee for Standard. English version of EN 1787, Brussels, Belgium (2000)
  16. CEN. Detection of irradiated food irradiated food containing crystalline sugar, method by ESR spectroscopy. European Committee for Standard. English version of EN 13708, Brussels, Belgium(2001)
  17. CEN. Detection of irradiated food irradiated food containing bone, method by ESR spectroscopy. European Committee for Standard. English version of EN 1786, Brussels, Belgium (1996)
  18. Origin. Origin Tutorial Manual. version 6.0. Microcal Software,Inc., Northampton, MA, USA. pp. 20-45 (1999)
  19. SAS. SAS Users Guide, SAS Institute Inc. Cary, NC, USA(1986)
  20. Raffi JJ, Benzaria SM. Identification of irradiated foods by electron spin resonance techniques. J. Radiat. Steril. 1: 281-304(1993)
  21. Delince$\acute{e}$ H. Detection of food treated with ionizing radiation.Trends Food Sci. Technol. 9: 73-82 (1998) https://doi.org/10.1016/S0924-2244(98)00002-8
  22. Stachowics W, Strzelozak-Burlinska G, Michalik J. Application of electron paramagnetic resonance (EPR) spectroscopy for control of irradiated food. J. Sci. Food Agr. 58: 407-415 (1992) https://doi.org/10.1002/jsfa.2740580316
  23. Alan O. Electron Spin Resonance Spectroscopy. Vol. 2. Academic Press, New York, NY, USA. pp. 1013-1031 (2001)
  24. Rhodes CJ, Thuy TT, Harry M. A determination of antioxidant efficiencies using ESR and computational methods. Spectrochim. Acta. A 60: 1401-1410 (2004) https://doi.org/10.1016/j.saa.2003.10.038
  25. Morsy MA, Khaled MM, Novel. EPR characterization of the anti-oxidant activity of tea leaves. Spectrochim. Acta A 58: 1271-1277 (2002) https://doi.org/10.1016/S1386-1425(01)00716-8
  26. Martin P, Vlasta B, Andrej S. Antioxidant properties of tea investigated by EPR spectroscopy. Biophys. Chem. 106: 39-56 (2003) https://doi.org/10.1016/S0301-4622(03)00159-5
  27. Jezierski A, Czechowski F, Jerzykiewicz M, Golonka I, Drozd J,Bylinska E, Chen Y, Seaward MRD. Quantitative EPR study on free radicals in the natural polyphenols interacting with metal ions and other environmental pollutants. Spectrochim. Acta A 58: 1293-1300 (2002) https://doi.org/10.1016/S1386-1425(01)00718-1
  28. Abdel-Fattah AA. Identification and dose assessment of irradiated cumin by EPR spectrometry. Appl. Radiat. Isotopes 56: 557-562(2002) https://doi.org/10.1016/S0969-8043(01)00246-9