Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지

Production of Piglet Derived from In Vitro Produced Porcine Early Embryos

돼지 초기배 체외수정란 이식으로 산자 생산

  • Choe, Chang-Yong (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Kim, Hyun-Jong (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Cho, Sang-Rae (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Yeon, Sung-Heum (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Han, Man-Hye (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Kim, Jae-Bum (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Kim, Sung-Jae (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Kang, Da-Won (Department of Physiology, College of Medicine and Institute of Health Sciences, Gyeongsang National University) ;
  • Son, Dong-Soo (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
  • 최창용 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 김현종 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 조상래 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 연성흠 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 한만희 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 김재범 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 김성재 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 강다원 (경상대학교 의과대학 생리학교실) ;
  • 손동수 (농촌진흥청 국립축산과학원 가축유전자원시험장)
  • Published : 2009.03.31
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Abstract

It is not easy for porcine embryos produced by in vitro systems to develop into blastocysts with high quality. To solve this problem, many researchers have developed novel culture methods. However, the formation of blastocysts with high quality is still low. In this study, we aimed to produce piglet following transfer of in vitro produced early embryos ($2{\sim}4$ cell stage embryos) or morula and blastocyst. The $2{\sim}4$ cell stage embryos were transferred to five estrus-synchronized recipients (200 embryos per recipient). One of the five sows farrowed three piglets, which contain two live piglets and one dead piglet, 114 days after embryo transfer. However, two recipients transferred with morula and blastocysts did not farrow. Microsatellite analysis confirmed that the genomic DNA of two live piglets were not genetically identical to that of the recipient. These results indicate that it is possible to obtain piglets by transfer of early embryos produced by in vitro production (IVP) systems.

Keywords

References

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