Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of glutamine and AgNO3 on plant regeneration of Sedum sarmentosum

돌나물의 식물체 분화에 미치는 Glutamine과 AgNO3의 영향

  • 안정호 (충남농업기술원) ;
  • 김현순 (농촌진흥청 국제기술협력과) ;
  • 이승엽 (원광대학교 생명자원과학연구소)
  • Published : 2009.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

This work was conducted to establish an efficient plant regeneration system for genetic transformation and the in vitro conservation of Sedum sarmentosum genetic resources. Effects of glutamine and $AgNO_3$ on plant regeneration between two genotypes were investigated using MS media supplemented with 0.2 mg/L NM and 3.0 mg/L BA. Calluses were formed on leaf explants placed on MS solid media supplemented with 3 mg/L 2,4-D and 1 mg/L BA. Calluses of Keumsan local strain produced shoots at a frequency of up to 100% after 50 days of culture on medium supplemented with glutamine. The highest number of shoots per callus was 17.6 at 350 mg/L glutamine. However, calluses of Wanju local strain gave rise to no shoots under the same culture conditions. Likewise, calluses of Keumsan local strain produced shoots at a frequency of up to 100% after 50 days of culture on medium supplemented with $AgNO_3$ whereas Wanju local strain sporadically produced shoots. The highest number of shoots per callus of Keumsan local stain was 16.1 at $15{\mu}M$ $AgNO_3$. Regenerated shoots were subcultured on hormone-free MS medium for rooting and shoot growth, and then 3-5 cm high plantlets were transplanted to the artificial soils comprising vermiculite and perlite, where they survived at a frequency of 88-100%. After being transplanted into upland soil:sand (1:1, v/v) in a greenhouse, regenerated plants showed a morphologically normal growth.

돌나물 (Sedum sarmentosum)의 형질전환 및 유전자원 기내보존을 위한 효율적인 식물체 분화 체계를 확립하기 위하여, 잎절편 유래의 켈러스로부터 식물체 분화에 미치는 glutamine과 $AgNO_3$의 영향과 분화 식물체의 순화 후 생존율을 조사하였다. 캘러스 유도는 3 mg/L 2,4-D와 1 mg/L BA, 식물체 분화는 0.2 mg/L NAA와 3.0 mg/L BA를 각각 첨가한 MS배지를 사용하였다. 금산 수집종에서 glutamine 첨가는 50일째에 100%의 식물체 분화를 보였으며, 350mg/L glutamine 첨가시 켈러스당 17.6개의 가장 많은 식물체가 분화되었다. $AgNO_3$ 첨가배지에서도 금산 수집종은 100% 식물체 분화를 보였으며, $15{\mu}M$ 첨가배지에서 캘러스당 16.1개의 식물체가 분화되어 가장 양호하였다. 완주 수집종은 glutamine 첨가에 관계없이 식물체가 전혀 분화되지 않았고, $5-15{\mu}M$ $AgNO_3$ 첨가배지에서만 소수의 식물체가 분화되었다. 분화 식물체는 생장조절제를 첨가하지 않은 MS 배지로 옮겨 shoot생장과 발근을 유도한 다음, vermiculite와 perlite를 혼합한 인공토양에서 순화시킨 결과 88-100%의 생존율을 보였다. 생존 식물체들은 강모래:밭흙 (1:1, v/v)을 혼합한 토양에 정식한 후에도 양호한 생장을 보였으며, 모식물과 형태적으로 차이가 없었다.

Keywords

References

  1. Ahn JH, Choe SS, Bae JH, Lee SY (2007) Effects of cutting date and bedsoil on root and shoot growth in autumn cutting of Sedum sarmentosum. J Bio-Environ Control 16:240-246
  2. Ahn JH, Lee SY (2004) Effects of growth regulators on callus induction and plant regeneration from leaf explants of Sedum sarmentosum. Kor J Plant Biotech 31:25-29 https://doi.org/10.5010/JPB.2004.31.1.025
  3. Ahn MS, Lim HC, Choi SR, Choi DC, Choi JS, Choi YG, Park YJ (2002) Effects of basal media, growth regulators and addition agents on callus formation and plant regeneration for anther culture of Hemerocallis spp. Kor J Hort Sd Technol 20:124-129
  4. Beyer EM Jr (1976) A potent inhibitor of ethylene action in plant. Physiol Plant 58:268-271 https://doi.org/10.1104/pp.58.3.268
  5. Chi GL, Pua EC (1989) Ethylene inhibitors enhanced de novo shoot regeneration from cotyledons of Brassica campestris ssp. chinensis (Chinese cabbage) in vitro. Plant Sci 64:243-250 https://doi.org/10.1016/0168-9452(89)90030-7
  6. Chraibi BK, Latch A, Roustan JP, Fallot J (1991) Stimulation of shoot regeneration form cotyledons of Helianthus annuus by the ethylene inhibitor, silver and cobalt. Plant Cell Rep 10:204-207
  7. Hatanaka T, Sawabe E, Azuma T, Uchida N, Yasuda T (1995) The role of ethylene in somatic ernbryogenesis from leaf discs of Coffea canephora. Plant Sci 107:199-204 https://doi.org/10.1016/0168-9452(95)04103-2
  8. Hyde CL, Phillips GC (1996) Silver nitrate promotes shoot development and plant regeneration of chile pepper (Capsicum annuum L.) via organogenesis. In Vitro Cell Dev Biol -Plant 32:72-80 https://doi.org/10.1007/BF02823134
  9. Jahne A, Becker D, Brettschneider R, Lorz H (1994) Regeneration of transgenic microspore-derived fertile barley. TAG 89:525-533
  10. Kim HJ, Bae JH, Lee SY (2007) Variation of fresh shoot quality by storage temperature after harvesting in local strain of Sedum sarmentosum. J Bio-Environ Control 16:240-246
  11. Kwack BH (1976) On the ecology of Sedum sarmentosum Bunge in Korea. J Kor Soc Hort Sci 17:69-77
  12. Larue TAG, Gamborg OL (1971) Ethylene production by plant cell cultures; Variations in production during growing cycle and bioassays with tobacco tissue cultures. Physiol Plant 15:437-497 https://doi.org/10.1111/j.1399-3054.1962.tb08047.x
  13. Lee SY, Ahn JH, Kim HJ (2006) Factors affecting shoot multiplication and rooting from cutting and in vitro node culture of Sedum sarmentosum. Kor J Hort Sci Technol 24:43-47
  14. Lee SY, Kim HJ, Bae JH, Shin JS, Lee SW (2007) Effect of shading on shoot growth and quality of Sedum sarmentosum in Korea. J Bio-Environ Control 16:388-394
  15. Lee SY, Kim TS, Kim HS (1988) Somatic embryogenesis of Bupleurum falcatum L. 1. Effects of growth regulator, glutamine and activated charcoal. Kor J Breed 20:191-198
  16. Lee SY, Yoo SO, Bae JH, Lee JH (2001) Effect of plant growth regulators on callus induction and plant regeneration of Farfugium japonica. Kor J Plant Biotech 29:45-49
  17. Lentini Z, Mussell H, Mutchler MA, Earle ED (1988) Ethylene generation and reversal of ethylene effects during development of in vitro rapid cycling Brassica campestris L. Plant Sci 54:75-81 https://doi.org/10.1016/0168-9452(88)90057-X
  18. Marton L, Browse J (1991) Facile transformation of Arabidopsis. Plant Cell Rep 10:235-239
  19. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  20. Murthy BNS, Singh RP, Saxena PK (1996) Induction of highfrequency somatic embryogenesis in geranium (Pelargonium x hortorum Bailey cv. Ringo Rose) cotyledonary cultures. Plant Cell Rept 15:423-426 https://doi.org/10.1007/BF00232068
  21. Olsen FL (1987) Induction of microspore embryogenesis in cultured anthers of Hordeum vulgare. The effects of ammonium nitrate, glutamine and asparagine as nitrogen sources. Carlsberg Res Commun 52:393-404 https://doi.org/10.1007/BF02907527
  22. Park KW, Kim YH (1997) Effect of different nutrient solutions on growth and quality in sedum (Sedum saramentosum) deep flow culture. Kor J Hort Sci Technol 15:152-153
  23. Purnhauser L, Medqyesy P, Czako M, Dix PJ, Marton L (1987) Stimulation of shoot regeneration in Triticum aestivum and Nicotiana plumbaginifolia viv. tissue culture using the ethylene inhibitor $AgNO_{3}$. Plant Cell Rep 6:1-4 https://doi.org/10.1007/BF00269725
  24. Taylor PWJ, Ko HL, Fraser TA, Masel N, Adkins SW (1994) Effect of silver nitrate on sugarcane cell suspension growth, Protoplast isolation, ethyIene production and shoot regeneration from cellsu spension cultures. J Exp Bot 45:1163-1168 https://doi.org/10.1093/jxb/45.8.1163
  25. Xu ZH, Sunderland N (1981) Glutamine, inositol and conditioning factor in the production of barley pollen callus in vitro. Plant Sci Lett 23:161-168 https://doi.org/10.1016/0304-4211(81)90007-9
  26. Yang SF (1985) Biosynthesis and action of ethylene. Hort Sci 20:41-45