Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Studies on the Specific Gene Expression in Lung Tissue of the Cloned Hanwoo

복제한우 폐조직에서 특이 유전자 발현에 관한 연구

  • Kim, Sang-Hwan (The Animal Biotechnology, GSBIT, Hankyong National University) ;
  • Chung, Duck-Won (The Animal Biotechnology, GSBIT, Hankyong National University) ;
  • Lee, Ho-Jun (Institute of Genetic Engineering, Hankyong National University) ;
  • Hwang, Sue-Yun (The Animal Biotechnology, GSBIT, Hankyong National University) ;
  • Min, Kwan-Sik (The Animal Biotechnology, GSBIT, Hankyong National University) ;
  • Yoon, Jong-Taek (The Animal Biotechnology, GSBIT, Hankyong National University)
  • 김상환 (한경대학교 생활환경정보통신 전문대학원 동물생명공학) ;
  • 정덕원 (한경대학교 생활환경정보통신 전문대학원 동물생명공학) ;
  • 이호준 (한경대학교 유전공학연구소) ;
  • 황수연 (한경대학교 생활환경정보통신 전문대학원 동물생명공학) ;
  • 민관식 (한경대학교 생활환경정보통신 전문대학원 동물생명공학) ;
  • 윤종택 (한경대학교 생활환경정보통신 전문대학원 동물생명공학)
  • Published : 2009.03.31
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Abstract

This study was conducted to investigate the specific expression genes in the cloned bovine tissues. Donor cells, cloned tissues were analysed by RAPD-RFLP method. The results were detected three genes (CH-U7B, CH-U7M and CH-U7P) in the cloned fetus. It was found a single copy genes by southern hybridization. Sequence analysis of CH-U7M gene was shown 99% homology to a previously reported EST from a cloned bovine fetus. The putative ORF was encode a protein of hydrophobicity index 0.03. Semi-quantitative RT-PCR by using the CH-LS001 specific primer was remarkably detected in the lung tissue of cloned fetus. Further investigation of these genes may provide one of the key information to explain the early death, abnormal fetus, large off-spring and the low pregnancy rate in the production of cloned bovine.

Keywords

References

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