Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
권호 표지
DOI QR코드

DOI QR Code

In Vitro Antilisterial Potential of a Marine Isolate of Aspergillus sp. Collected from the South Coast of Korea

  • Published : 2009.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study was carried out to assess the antilisterial potential of ethyl acetate (EtOAc) extract of a marine isolate of Aspergillus sp. The in vitro antilisterial efficacy of ethyl acetate extract was examined using disc diffusion, minimum inhibitory concentration (MIC) determination, cell viable count and scanning electron microscopic (SEM) methods against the employed strains of Listeria monocytogenus. The ethyl acetate extract ($300{\mu}g\;disc^{-1}$) exhibited a promising antilisterial effect as diameters of inhibition zones against L. monocytogenes ATCC 19111, 19116, 19118, 19166 and 15313, which were found in the range of 11-17 mm along with their MIC values ranging from 125 to $1000{\mu}g\;ml^{-1}$ respectively. Also the EtOAc extract had strong detrimental effect on the viable count of the tested L. monocytogens ATCC 19166. Furthermore, scanning electron microscopic (SEM) study demonstrated potential detrimental effect of ethyl acetate extract on the morphology of L. monocytogenes ATCC 19116 at the used MIC concentration. These findings strongly support the role of ethyl acetate extract of a marine isolate of Aspergillus sp. as an antiliterial potential.

Keywords

References

  1. Montville, T. J., Winkowski, K., and Ludescher, R. D. (1995) Models and mechanisms for bacteriocin action and application. Int. Dairy J. 5, 797-814 https://doi.org/10.1016/0958-6946(95)00034-8
  2. Farber, J. M., and Peterkin, P. I. (1991) Listeria monocytogenes, a food-borne pathogen. Microbial. Rev. 55, 476-511
  3. Broseta, S. M., Diot, A., Bastian, S., Riviere, J., and Cerf, O. (2003) Estimation of low bacterial concentration: Listeria monocytogenes in raw milk. Int. J. Food Microbiol. 80, 1-15 https://doi.org/10.1016/S0168-1605(02)00117-4
  4. Dalton, C. B., Austin, C. C., Sobel, J. P., Hayes, S., Bibb, W. F., Graves, L. M., Swaminathan, B., Proctor, M. E., and Griffin, P. M. (1997) An outbreak of gastroenteritis and fever due to Listeria monocytogenes in milk. N. Engl. J. Med. 336, 100-105 https://doi.org/10.1056/NEJM199701093360204
  5. Salamina, G. E., Donne, D., Nicolini, A., Poda, G., Cesarone, D., Bucci, M., Fini, R., Maldini, M., Schuchat, A., Swaminathan, B., Bibb, W., Rocourt, J., Binkin, N., and Salmaso, S. (1996) A foodborne outbreak of gastroenteritis involving Listeria monocytogenes. Epidemiol. Infect. 117, 429-436 https://doi.org/10.1017/S0950268800059082
  6. Fenlon, D. R. (1999) Listeria monocytogenes in the natural environment. In: Ryser ET, Marth EH, eds. Listeria, listeriosis, and food safety, 2nd eds. New York: Marcel Dekker Inc
  7. Vazquez-Boland, J. A., Kuhn, M., Berche, P., Chakraborty, T., Dominguez- Bernal, G.., Goebel, W., Gonzalez-Zorn, B., Wehland, J., and Kreft, J. (2001) Listeria pathogenesis and molecular virulence determinants. Clin. Microbiol. Rev. 14, 584-640 https://doi.org/10.1128/CMR.14.3.584-640.2001
  8. Bille, J. (1990) Epidemiology of human listeriosis in Europe, with special reference to the Swiss outbreak. In: Miller AJ, Smith JL, Somkuti GA, eds. Foodborne listeriosis. New York: Elsevier
  9. Schlech, W. F. III (2000) Foodborne listeriosis. Clin. Infect. Dis. 31, 770-775 https://doi.org/10.1086/314008
  10. Shen, H. C., Tato, M., and Fan, X. (1998) Listeria monocytogenes as a probe to study cell-mediated immunity. Curro Opin. Immunol. 10, 450-458 https://doi.org/10.1016/S0952-7915(98)80120-9
  11. Martinez, R. C. R., and De Martinis, E. C. P. (2005) Antilisterial activity of a crude preparation of Lactobacillus sakei 1 bacteriocin and its lack of influence on Listeria monocytogenes haemolytic activity. Food Control 16, 429-433 https://doi.org/10.1016/j.foodcont.2004.05.002
  12. Faulkner, D. J. (2001) Marine natural products. Nat. Prod. Rep. 18, 1-49 https://doi.org/10.1039/b006897g
  13. Bajpai, V. K., and Kang, S. C. (2008) The antidermatophytic potential of the marine isolate of Aspergillus sp. collected from South Coast of Korea. K. J. Environ. Agric. 27(1), 80-85 https://doi.org/10.5338/KJEA.2008.27.1.080
  14. Murray, P. R., Baron, E. J., Pfaller, M. A., Tenover, F. C., and Yolke, R. H. (1995) Manual of Clinical Microbiology, Vol. 6, eds. Washington: ASM Press
  15. Chandrasekaran, M., and Venkatesalu, V. (2004) Antibacterial and antifungal activity of Syzygium jambolanum seeds. J. Ethnopharmacol. 91, 105-108 https://doi.org/10.1016/j.jep.2003.12.012
  16. Shin, S. Y., Bajpai, V. K., Kim, H. R., and Kang, S. C. (2007) Antibacterial activity of eicosapentaenoic acid (EPA) against foodbome and food spoilage microorganisms. LWT 40, 1515-1519 https://doi.org/10.1016/j.lwt.2006.12.005
  17. Kockro, R. A., Hampl, J. A., Jansen, B., Peters, G.., Scheihing, M., and Giacomelli, R. (2000) Use of scanning electron microscopy to investigate the prophylactic efficacy of rifampin-impregnated CSF shunt catheters. J. Med. Microbiol. 49, 441-450 https://doi.org/10.1099/0022-1317-49-5-441
  18. Koyama, S., Yamaguchi, Y., Tanaka, S., and Motoyashiya, J. (1997) A new substance (yoshixol) with an interesting antibiotic mechanism from wood oil of Japanese traditional tree (kiso-hinoki), Chamaecyparis obtuse. Gen. Pharmacol. 28, 797-804 https://doi.org/10.1016/S0306-3623(96)00370-9
  19. Song, H. J., and Richard, J. (1997) Antilisterial activity of three bacteriocins used at sub minimal inhibitory concentrations and cross-resistance of the survivors. Int. J. Food Microbiol. 36, 155-161 https://doi.org/10.1016/S0168-1605(97)01254-3
  20. Roxanne, J. O., and Enzo, A. P. (2005) Antilisterial activity of ethanolic extracts of medicinal plants, Eremophila alternifolia and Eremophila dutton ii, in food homogenates and milk. Food Control 18, 387-390 https://doi.org/10.1016/j.foodcont.2005.11.004