한국동물위생학회지 (Korean Journal of Veterinary Service)

  • 제32권4호
  • /
  • Pages.335-341
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  • 2009
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  • 3022-7372(pISSN)
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  • 2287-7630(eISSN)
한국동물위생학회 (The Korean Society of Veterinary Service)
권호 표지

개 혈액 재료에서의 Brucella 검출을 위한 진단방법의 비교

Comparison of diagnostic methods for detection of Brucella species in dog blood samples

  • Kwon, Soon-Oh (College of Veterinary Medicine, Kangwon National University) ;
  • Lam, Truong Quang (College of Veterinary Medicine, Kangwon National University) ;
  • Her, Moon (National Veterinary Research and Quarantine Service) ;
  • Ahn, Dong-Chun (College of Veterinary Medicine, Chonbuk National University) ;
  • Park, Sang-Hee (National Institute of Health) ;
  • Park, Mi-Yeoun (National Institute of Health) ;
  • Lee, Young-Ju (College of Veterinary Medicine, Kyuongpook National University) ;
  • Hahn, Tae-Wook (College of Veterinary Medicine, Kangwon National University)
  • 발행 : 2009.12.30
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초록

Canine brucellosis produce abortions and infertility in dogs and is currently diagnosed by serological methods such as rapid slide agglutination test with 2-mercaptoethanol (2-ME RSAT) and immunochromatographic assay (ICA). Bacterial isolation is considered gold standard for Brucella diagnosis and the polymerase chain reaction (PCR) is an alternative method to bacterial isolation. A total of 36 whole blood samples were collected from dogs reared in area of Chuncheon and were subjected to serology (2-ME RSAT and ICA for B. canis, Rose Bengal test and C-ELISA for B. abortus), blood culture and 3 types of PCRs (BSCP31, 16s rRNA, and OMP-2). All blood samples were negative by serology and blood cultures. The BCSP31 and the OMP-2 PCR detected 5 samples were positive whereas the 16S rRNA PCR detected all samples were negative as serological methods and blood culture did. From the results observed in the present study, we conclude that 16S rRNA PCR could be used for direct PCR for canine blood samples.

키워드

참고문헌

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