KSBB Journal

  • 제24권5호
  • /
  • Pages.489-494
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  • 2009
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  • 1225-7117(pISSN)
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  • 2288-8268(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

유채대의 이단 고온 처리에 의한 알콜 발효용 당화물 생산

Two-step High Temperature Pretreatment Process for Bioethanol Production from Rape Stems

  • 한재건 (강원대학교 BT특성화 학부대학) ;
  • 오성호 (강원대학교 BT특성화 학부대학) ;
  • 정명훈 (강원대학교 BT특성화 학부대학) ;
  • 김승섭 (강원대학교 BT특성화 학부대학) ;
  • 서현범 (충주대학교 바이오산업학과) ;
  • 정경환 (충주대학교 바이오산업학과) ;
  • 장영석 (농촌진흥청 작물과학원 목포시험장) ;
  • 김일철 (전남대학교 생물학과) ;
  • 이현용 (강원대학교 BT특성화 학부대학)
  • Han, Jae-Gun (College of Bioscience & Biotechnology, Kangwon National University) ;
  • Oh, Sung-Ho (College of Bioscience & Biotechnology, Kangwon National University) ;
  • Jeong, Myoung-Hoon (College of Bioscience & Biotechnology, Kangwon National University) ;
  • Kim, Seung-Seop (College of Bioscience & Biotechnology, Kangwon National University) ;
  • Seo, Hyeon-Beom (Department of Biotechnology, Chungju National University) ;
  • Jeong, Kyung-Hwan (Department of Biotechnology, Chungju National University) ;
  • Jang, Young-Seok (Mokpo Experiment Station, National Institute of Crop Science, RDA) ;
  • Kim, Il-Cheol (Department of Biology, Chonnam National University) ;
  • Lee, Hyeon-Yong (College of Bioscience & Biotechnology, Kangwon National University)
  • 발행 : 2009.10.29
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초록

우리 연구팀은, 농산부산물인 유채대의 알콜 발효용 당화물 생산 가능성을 연구하였다. 이를 위해 농산부산물인 유채대를 연속적으로 5 mL/min의 속도로 산을 이용하지 않고 증류수만을 이용해 이단 고온 처리 ($200^{\circ}C$ and 15 Mpa, $375^{\circ}C$ and 23 Mpa)하였다. 본 전처리 공정을 통한 가수수분해물의 당화물 생성은 최종적으로 자일로스와 글루코스의 경우 25.6 g/L, 5.5 g/L가 생성되었다. 이는 유채대에 존재하는 글루코스와 자일로스의 초기 양 대비 각각 18%와 59%의 전환 수율을 나타낸다. 또한 이 공정은 타 공정들에 비하여 대표발효 저해 산물인 HMF의 생성량이 0.2 ppm으로 극히 낮은 수치를 보였으며, 가수분해물의 에탄올 생산시, 글루코스의 발효를 통한 에탄올 생성 전환수율이 90% 이상으로 높은 생성율을 보였다. 따라서 본 공정을 통해 다른 농산부산물이나 해조류 전체에 응용된다면, 고 수율의 에탄올 생산용 당화물을 생산할 것으로 예상한다.

Two-step pretreatment process was investigated to efficiently hydrolyzed rape stems for obtaining fermentable sugars. The process was consisted of two consecutive steps as $200^{\circ}C$ and 15 MPa and $374^{\circ}C$ and 24 MPa with the flow rate of 5 mL/min. Under this condition, 5.5 (g/L) of glucose and 25.6 (g/L) of xylose were obtained from rape stems, showing 18% of glucose yield based on 25% cellulose in the rape stems. It was also found that this process could generate less amounts of toxic residues, such as HMF (Hydroxy- Methyl-Furfural) and other fulfural components during hydrolysis process. It could reaction maintain relatively high ethanol production yield as 90% of theoretical conversion yield from glucose. Therefore, this pretreatment process could be applied to hydrolyze other cellulosic and marine resources such as woods, stem and algae for bioethanol production.

키워드

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