Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Acid Production and Phytate Degradation using a Leuconostoc mesenteroides KC5l Strain in Saccharified-Rice Suspension

현미 당화액에서 Leuconostoc mesenteroides KC51 균주에 의한 산의 생성과 Phytate의 분해

  • In, Man-Jin (Department of Human Nutrition and Food Science, Chungwoon University) ;
  • Choi, Seo-Yeon (Department of Human Nutrition and Food Science, Chungwoon University) ;
  • Kim, Hye-Rim (Department of Human Nutrition and Food Science, Chungwoon University) ;
  • Park, Dan-Bi (Department of Human Nutrition and Food Science, Chungwoon University) ;
  • Oh, Nam-Soon (Department of Food Science and Technology, Kongju National University) ;
  • Kim, Dong-Chung (Institute of Basic Science, Sungkyunkwan University)
  • 인만진 (청운대학교 식품영양학과) ;
  • 최서연 (청운대학교 식품영양학과) ;
  • 김혜림 (청운대학교 식품영양학과) ;
  • 박단비 (청운대학교 식품영양학과) ;
  • 오남순 (공주대학교 식품공학과) ;
  • 김동청 (성균관대학교 기초과학연구소)
  • Published : 2009.03.31
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Abstract

A saccharified-rice was fermented using Leuconostoc(Ln.) mesenteroides KC51 strain in various dry matter (DM) contents (4%, 8%, and 12%) at $30^{\circ}C$ for 18 h. The changes of viable cell number, acid production and phytate degradation in saccharified-rice during fermentation were investigated. The viable cell population of Ln. mesenteroides KC51 was increased rapidly in proportion to DM contents during the 9 h of cultivation. The changes of pH and titratable acidity in saccharified-rice were dependent on DM contents. At high DM content (12%), the viable cell number of Ln. mesenteroides KC51 increased to 9.56 log CFU/g after 6 h of fermentation. The pH and titratable acidity reached to pH 3.38 and 0.93% after 18 h of fermentation, respectively. The phytate, known as an antinutrient factor, in saccharified-rice was degraded by Ln. mesenteroides KC51 cultivation. The decrease of phytate during fermentation approximately coincided with the increase of Ln. mesenteroides KC51 population observed in fermented saccharified-rice. Regardless of DM contents, the levels of phytate were reduced to around 50% of initial concentration.

쌀과 유산균을 이용한 곡류 발효 식품을 개발하기 위하여 김치에서 분리한 유산균 Ln. mesenteroides KC51를 호화, 액화, 당화시킨 현미 분말 현탁액에 배양하였다. 멸균한 현미 당화물에 KC51 균주를 접종하고 $30^{\circ}C$에서 진탕 배양한 결과, 생균수는 당화물의 농도 증가에 따라서 접종 후 9시간까지 급격히 증가하였으며 그 이후 완만하게 감소하였다. 당화물 농도가 12% 일 때 Ln. mesenteroides KC51 균주의 생육은 9.56 log CFU/g까지 증가하였다. 적정산도는 당화물의 농도가 증가함에 따라서 증가하였으며, 당화물의 농도가 12%에서 배양 18시간 째 0.93%까지 증가하였으며, pH는 3.38로 감소하였다. 이때 젖산과 초산의 함량이 각각 0.395%와 0.242%로 대략 3:2의 생성비율을 보였다. 현미 배양액 중 항영양인자인 phytate의 농도는 유산균의 증식에 따라 감소되었으며, Ln. mesenteroides KC51 균주의 최대 phytate 분해율은 당화물의 농도와 무관하게 $45{\sim}47%$를 나타내었다.

Keywords

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