Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Inhibitory Effect on the Lipopolysaccharide-Induced Nitric Oxide Formation in RAW264.7 and on the Proliferation of HeLa Cell Lines by the Stem Bark Extracts of Abies nephrolepis

분비나무 줄기 수피 추출물에 의한 HeLa Cell Line의 증식억제 효과와 RAW264.7 세포에서 Lipopolysaccharide에 의해 유도된 Nitric Oxide 생성 저해효과

  • Published : 2009.03.31
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Abstract

Abies nephrolepis(Pinaceae) extracts were tested for determined immune system regulating activity based on antiinflammatory activity, antioxidant activity and anti-proliferative effect on HeLa cell line. The A. nephrolepis extracts increased dose-dependently anti-proliferation of HeLa cell line. The DM fraction of the extracts having anti-proliferatative effects of HeLa cell line was fractionalized four subfractions($D1{\sim}D4$). Inflammation-induced NO production was inhibited by D2 and D4 in LPS-activated RAW264.7 macrophages. And also, this fractions showed antioxidant activity examined by DPPH radical scavenging effects. These results suggest that the potential use of DM fraction of A. nephrolepis in chemoprevention and regulation overproduction of NO on pathogenic conditions. The mechanism of the inflammatory effects, however, must be evaluated through various parameters in the induction cascade of NO production.

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