Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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The Effect of the Injinhotang Extract on the Hepatocarcinogenesis Induced by N-Nitrosodiethylamine and Carbon Tetrachloride in Rats

N-Nitrosodiethylamine과 사염화탄소로 유발된 흰쥐의 간암발생에 대한 인진호탕 추출액의 효과

  • Yoon, Jung-Sik (Dept. of Anatomy, College of Oriental Medicine, Dongshin University) ;
  • Kim, Jeong-Sang (Dept. of Anatomy, College of Oriental Medicine, Dongshin University)
  • 윤중식 (동신대학교 한의과대학 해부학교실) ;
  • 김정상 (동신대학교 한의과대학 해부학교실)
  • Published : 2009.04.30
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Abstract

In order to examine the effect of Injinhotang extract on the hepatocarcinogenesis induced by N-nitrosodiethylamine (NDEA) and carbon tetrachloride ($CCl_4$) in 8 week-old rats. Experimental rats were subdivided into three groups; normal group (Nor), hepatic cancer inducing control group (Con), and control group administered Injinhotang extract 260 mg/kg/day (IJH). The body weight decreased significantly (p<0.05) in the Con compared with the Nor. The body weight of IJH group more increased than Con. Rats intoxicated with NDEA had significantly (p<0.05) increased levels of serum AST, ALT, LDH, ALP, and AFP. On the contrary, group treated with Injinhotang extract had inhibited levels of serum AST, ALT, LDH, ALP, and AFP. The bcl-2 mRNA expression levels in rat liver were more increased in the IJH than Con, but these levels of c-myc mRNA were more decreased in the IJH than Con. Also, cytoplasmic vacuolizations in the liver of NDEA-administrated rats were inhibited by the treatment of Injinhotang extract. These results suggest that administration of Injinhotang extract suppresses or retards NDEA and $CCl_4$-induced hepatocarcinogenesis in rats.

본 실험은 N-nitrosodiethylamine(NDEA)와 사염화탄소 ($CCl_4$)로 유발된 흰쥐의 간암 생성에 대한 인진호탕의 효과를 조사하였다. 실험은 8주 동안 실시하였고, 세 군으로 분류하였다. 정상군(Nor), 간암을 유발한 대조군(Con), 간암 유발 후 인진호탕 추출액(260 mg/kg/day)을 투여한 실험군 (IJH)으로 구분하였다. 체중은 정상군에 비하여 대조군에서 유의적으로 감소하였지만 실험군에서는 증가하였다. NDEA로 유발된 흰쥐의 혈청 AST, ALT, LDH, ALP와 AFP의 수준은 증가하였다. 반대로, 인진호탕 추출액을 처리했을 때, 혈청 AST, ALT, LDH, ALP와 AFP의 수준이 감소하였다. 흰쥐의 간에서 bcl-2 mRNA의 수준은 대조군에 비해 실험군에서 증가되었다. 그러나 c-myc mRNA의 수준은 대조군에 비해 감소하였다. 또한 NDEA로 손상된 간세포 조직의 공포화가 인진호탕 추출액의 투여로 감소하는 것을 관찰 할 수 있었다. 이상의 결과로 보아 인진호탕 추출액 투여가 NDEA와 사염화탄소로 유도된 흰쥐의 간암 생성을 억제 또는 지연하는 것으로 사료된다.

Keywords

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