Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Metabolism of Wogonoside by Human Fecal Microflora and Its Anti-pruritic Effect

  • Trinh, Hien-Trung (Department of Pharmaceutical Science and Department of Life and Pharmaceutical Sciences, Kyung Hee University) ;
  • Jang, Seo-Young (Department of Food and Nutrition, Kyung Hee University) ;
  • Han, Myung-Joo (Department of Food and Nutrition, Kyung Hee University) ;
  • Kawk, Ho-Young (Graduate School of Biotechnology and PMRC, Kyung Hee University) ;
  • Baek, Nam-In (Graduate School of Biotechnology and PMRC, Kyung Hee University) ;
  • Kim, Dong-Hyun (Department of Pharmaceutical Science and Department of Life and Pharmaceutical Sciences, Kyung Hee University)
  • Published : 2009.04.30
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Abstract

To understand the relationship between the metabolism of wogonoside from the rhizome of Scutellaria baicalensis, and its anti-pruritic effect, we anaerobically incubated it with human fecal microflora, identified its metabolite identified, and investigated its anti-pruritic effect in compound 48/80 or histamineinduced pruritic mice. Wogonoside was metabolized to wogonin, with metabolic activity of $6.9{\pm}5.1\;nmol/h/mg$ wet weight of fecal microflora. Orally administered wogonoside had more potent anti-scratching behavioral effect in compound 48/80 or histamine-treated mice than intraperitoneally treated one, apart from orally administered its metabolite, wogonin, which was more potent than the orally administered one. Wogonoside showed more potent anti-pruritic effects when administered at 5 h prior to the pruritic agent treatment than when administered at 1 h before. However, wogonin orally administered 1 h before the treatment with pruritic agents showed a more potent anti-pruritic effect than when treated at 5 h before. Orally administered wogonoside may be metabolized to wogonin in the intestine and its anti-scratching behavioral effect may be dependent on its metabolism by intestinal microflora.

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References

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