Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Anti-oxidative Properties of Lipids Extracted from Rainbow Trout (Oncorhynchus mykiss) Fed with Carotenoids and Conjugated Linoleic Acid

  • Eum, Gwang-Sik (Dept. of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University) ;
  • Kim, Jeong-Gyun (Dept. of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University) ;
  • Ha, Yeong-Lae (Divison of Applied Life Science, Gyeongsang National University) ;
  • Park, Chan-II (Dept. of Marine Life Science/Institute of Marine Industry, Gyeongsang National University) ;
  • Kang, Seok-Joong (Dept. of Marine Life Science/Institute of Marine Industry, Gyeongsang National University) ;
  • Choi, Yeung-Joon (Dept. of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University) ;
  • Choi, Byeong-Dae (Dept. of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University)
  • Published : 2009.12.31
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Abstract

A commercial diet supplemented with carotenoids and conjugated linoleic acid was fed to rainbow trout (Oncorhynchus mykiss) for 8 weeks. To investigate the anti-oxidative properties of these compounds, lipids from the muscle and viscera of the fish were subjected to different assays. At $10\;{\mu}g/mL$, L-ascorbic acid exhibited 95% 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity, while the tissue lipids showed little radical scavenging activity. At 50 and $100\;{\mu}g/mL$, the lipids of the muscles and viscera showed 11.7-22.6% and 11.3-24.9% DPPH radical-scavenging activity, respectively. A lipid peroxidation inhibitory assay using the ferric thiocyanate method was also performed in comparison with $\alpha$-tocopherol at a concentration of 6.0 mg/mL. Our results indicate that the anti-oxidative property of the lipids in fish muscle, which was 85.2% compared to 85.3% for the visceral lipids, was stronger than that of $\alpha$-tocopherol (74.3%) following 3 days of storage at $40^{\circ}C$.

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