한국가금학회지 (Korean Journal of Poultry Science)

  • 제36권1호
  • /
  • Pages.85-88
  • /
  • 2009
  • /
  • 1225-6625(pISSN)
  • /
  • 2287-5387(eISSN)
한국가금학회 (The Korean Society of Poultry Science)
권호 표지
DOI QR코드

DOI QR Code

한국재래닭 COI 유전자의 단일염기다형 분석

Single Nucleotide Polymorphism Analysis of the COI Gene in Korean Native Chicken

  • Jin, S.D. (National Science Museum) ;
  • Seo, D.W. (Division of Animal Science and Resources, Chungnam National University) ;
  • Sim, J.M. (Division of Animal Science and Resources, Chungnam National University) ;
  • Baek, W.K. (National Science Museum) ;
  • Jung, K.C. (Division of Animal Science and Resources, Chungnam National University) ;
  • Jang, B.K. (National Institute of Animal Science, R.D.A.) ;
  • Choi, K.D. (The Graduate School of Bio & Information Technology, Hankyong National University) ;
  • Lee, J.H. (Division of Animal Science and Resources, Chungnam National University)
  • 발행 : 2009.03.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

조류에서 미토콘드리아내 유전자인 cytochrome c oxidase I(COI)는 종 구분을 위한 바이오마커로 많이 이용이 되고 있다. 본 연구는 이 유전자의 변이를 이용하여 닭의 품종 구분이 가능한지를 실험하기 위하여 실시하였다. 그 결과 한국 재래닭은 공시축으로 사용한 산란계인 White Leghorn과 육계인 Cornish가 가지고 있는 단일염기다형을 모두 가지고 있는 것으로 판명되어 품종구분을 위한 마커로 사용하기에는 적합하지 않은 것으로 확인되었다. 그러나 본 연구 결과를 통하여 한국재래계가 육계와 산란계의 특성을 모두 가지고 있다는 기존의 학설을 뒷받침하였으며, 품종 구분을 위하여 미토콘드리아와 genomic DNA 유래 단일염기다형 선발의 중요성을 확인할 수 있었다.

One of the mitochondrial genes, called cytochrome c oxidase I (COI), has been widely used for the species identification (called bio-barcode) in birds. In this study, the bio-barcode has been applied to chicken breeds in Korea whether it also can be used as a molecular marker for breed identification. Data indicated that Korean native chicken has the mixed SNP (single nucleotide polymorphism) patterns between White Leghorn (Layer) and Cornish (Broiler) and ultimately, it can not be used as the marker for breed identification. However, this result indicates the mixed use of the Korean native chicken, since it has been used for dual purpose for producing meat and egg for a long time. In order to use as a marker for species identification, more reliable mitochondrial and/or nuclear DNA markers need to be developed.

키워드

참고문헌

  1. Crawford RD 1990 Origin and history of poultry species. In: Poultry Breeding and Genetics (Ed. R. D. Crawford) Elsevier. pp7-8
  2. Dawnay N, Ogden R, McEwing R, Carvalho GR, Thorpe RS 2007 Validation of the barcoding gene COI for use in forensic genetic species identification. Forensic Sci Int 173(1):1-6 https://doi.org/10.1016/j.forsciint.2006.09.013
  3. Hebert PDN, Cywinska A, Ball SL, DeWaard JR 2003 Biological identifications through DNA barcodes. Proc R Soc Lond B Biol Sci 270:313-321 https://doi.org/10.1098/rspb.2002.2218
  4. Hebert PDN, Stoeckle MY, Zemlak TS, Francis CM 2004 Identification of birds through DNA barcodes. PLoS Biol 2:e312 https://doi.org/10.1371/journal.pbio.0020312
  5. Kumar S, Tamura K, Nei M 2004 MEGA 3.1: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinformatics 5:150-163 https://doi.org/10.1093/bib/5.2.150
  6. Liu YP, Wu GS, Yao YG, Miao YW, Luikart G, Baig M, Pereira AB, Ding ZL, Palanichamy MG, Zhang YP 2006 Multiple maternal origins of chickens: Out of the Asian jungles. Mol Phylogenet Evol 38(1):12-19 https://doi.org/10.1016/j.ympev.2005.09.014
  7. Liu ZG, Lei CZ, Luo J, Ding C, Chen GH, Chang H, Wang KH, Liu XX, Zhang XY, Xiao XJ, Wu SL 2004 Genetic variability of mtDNA sequences in Chinese native chicken breeds. Asian-Aust J Anim Sci 17(7):903-909 https://doi.org/10.5713/ajas.2004.903
  8. Niu D, Fu Y, Luo J, Ruan H, Yu XP, Chen G, Zhang YP 2002 The origin and genetic diversity of Chinese native chicken breeds. Biochem Genet 40:163-174 https://doi.org/10.1023/A:1015832108669
  9. Wallis JW, Aerts J, Groenen MA, Crooijmans RP, Layman D, Graves TA, Scheer DE, Kremitzki C, Fedele MJ, Mudd NK, Cardenas M, Higginbotham J, Carter J, McGrane R, Gaige T, Mead K, Walker J, Albracht D, Davito J, Yang SP, Leong S, Chinwalla A, Sekhon M, Wylie K, Dodgson J, Romanov MN, Cheng H, de Jong PJ, Osoegawa K, Nefedov M, Zhang H, McPherson JD, Krzywinski M, Schein J, Hillier L, Mardis ER, Wilson RK, Warren WC 2004 A physical map of the chicken genome. Nature 432(7018):761-764 https://doi.org/10.1038/nature03030
  10. Yoo HS, Eah JY, Kim JS, Kim YJ, Min MS, Paek WK, Lee H, Kim CB 2006 DNA barcoding Korean birds. Mol Cells 22(3):323-327
  11. Yoon D, Kwon YS, Lee KY, Jung WY, Sasazaki S, Mannen H, Jeon JT, Lee JH 2008 Discrimination of Korean cattle (Hanwoo) using DNA markers derived from SNPs in bovine mitochondrial and SRY genes. Asian-Aust J Anim Sci 21(1):25-28 https://doi.org/10.5713/ajas.2008.70209
  12. 오재돈 박미현 공홍식 이학교 전광주 연성흠 상병돈 최철환 조병욱 2005 한국 재래 닭 품종 특성 및 초기성장 개량을 위한 분자표지 개발. 한국가금학회지 32(1): 29-34

피인용 문헌

  1. Single nucleotide polymorphism analysis of Korean native chickens using next generation sequencing data vol.42, pp.2, 2015, https://doi.org/10.1007/s11033-014-3790-5
  2. The breeding history and commercial development of the Korean native chicken vol.73, pp.01, 2017, https://doi.org/10.1017/S004393391600088X
  3. DNA Markers for the Genetic Diversity in Korean Native Chicken Breeds: A Review vol.43, pp.2, 2016, https://doi.org/10.5536/KJPS.2016.43.2.63