한국가금학회지 (Korean Journal of Poultry Science)

  • 제36권3호
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  • Pages.207-213
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  • 2009
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  • 1225-6625(pISSN)
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  • 2287-5387(eISSN)
한국가금학회 (The Korean Society of Poultry Science)
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한국재래닭의 ADSL 유전자 내 단일염기변이를 이용한 경제형질과의 연관성 분석

Identification of Novel Single Nucleotide Polymorphisms on ADSL Gene Using Economic Traits in Korean Native Chicken

  • 이진아 (한경대학교 유전정보연구소) ;
  • 전세아 (한경대학교 유전정보연구소) ;
  • 오재돈 (한경대학교 유전정보연구소) ;
  • 박경도 (한경대학교 유전정보연구소) ;
  • 최강덕 (한경대학교 유전정보연구소) ;
  • 전광주 (한경대학교 유전정보연구소) ;
  • 이학교 (한경대학교 유전정보연구소) ;
  • 공홍식 (한경대학교 유전정보연구소)
  • Lee, J.A. (Genomic Informatics Center, Hankyong National University) ;
  • Jeon, S.A. (Genomic Informatics Center, Hankyong National University) ;
  • Oh, J.D. (Genomic Informatics Center, Hankyong National University) ;
  • Park, K.D. (Genomic Informatics Center, Hankyong National University) ;
  • Choi, K.D. (Genomic Informatics Center, Hankyong National University) ;
  • Jeon, G.J. (Genomic Informatics Center, Hankyong National University) ;
  • Lee, H.K. (Genomic Informatics Center, Hankyong National University) ;
  • Kong, H.S. (Genomic Informatics Center, Hankyong National University)
  • 발행 : 2009.09.30
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초록

퓨린 합성의 반응을 촉진시키며 뇌기능장애, 성장장애 그리고 에너지대사에 핵심적인 역할을 하는 ADSL(Adenylosuccinate lyase)의 exon 영역을 중심으로 PCR을 수행하여 DNA 염기서열 분석을 통해 한국재래닭에서 단일염기다형성을 확인하였다. 염기분석 결과 총 11개(intron 5: T7724C, C7732T intron 8: G10108T intron 9: A10356T, G10375A, A10402 intron 10: A12716T, T12717A intron 12: C15491T exon 13: C15542T, C15550T)를 확인할 수 있었으며 특히 exon 13지역의 변이들은 각각 아미노산이 바뀌는 missense mutation 임이 확인되었다(Alanine$\rightarrow$Valine, Proline$\rightarrow$Serine). 또한 C15542T 변이는 NCBI의 SNP 데이터베이스에 등록된 것으로 확인되었고, C15550T는 SNP 데이터베이스에 등록되지 않은 신규 변이지역으로 확인되었다. 이는 단백질 발현이 향상되는 3'UTR 지역 근처인 exon 13 부위이며 추가적으로 ADSL 유전자의 아미노산 변이가 닭 집단의 성장 및 에너지 대사와의 연관성 검증을 통해 본 연구 결과는 중요하게 활용될 것으로 기대된다.

Adenylosuccinate lyase (ADSL) deficiency is a disease of purine metabolism which affects patients both biochemicall and behaviorally. An obstacle of this purine nucleotide cycle(PNC) can be caused brain functional disorder and growth disorder. So ADSL deficiency, which is associated with sever mental retardation, autistic features and energy metabolism. This study was performed to identify SNP on ADSL gene in chicken. The nucleotides were observed as T to C ($7724^{th}$ nucleotide), C to T ($7732^{nd}$ nucleotide), G to T ($10108^{th}$ nucleotide), A to T ($10356^{th}$ nucleotide), G to A($10375^{th}$ nucleotide), A to C ($10402^{nd}$ nucleotide), A to T ($12716^{th}$ nucleotide), T to A ($12717^{th}$ nucleotide), C to T ($15491^{st}$ nucleotide), C to T ($15542^{nd}$ nucleotide) and C to T ($15550^{th}$ nucleotide). The nucleotide substitutions at $15542^{nd}$ and $15550^{th}$ (GeneBank accession no. AY665559) were found as missense mutation (alanine$\rightarrow$valine, proline$\rightarrow$serine, respectively). This study will be useful for farther researches for identifying association between these SNPs and energy metabolism in chicken. The C15550T SNP showed three genotypes, CC, CT, TT by digestion with the genotype TT had significantly faster the first lay day (150.0) than CT (162.0, P<0.05) and genotype TT (150.0, P<0.05) had significantly higher the egg production rate than CT (172.4, P<0.05). According to result of this study, a C15550T was found to have a significantly effect first lay day and mean egg production. It will be possible to use SNP marker on selecting chicken to improve important economic traits, which is the first lay day and mean egg production.

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참고문헌

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피인용 문헌

  1. ) vol.59, pp.5, 2018, https://doi.org/10.1080/00071668.2018.1493188