Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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Optimization of Conditions for Isolating and Cultivating Bacillus sp. Se-103 with a Mesophilic Feather-Degrading Activity

중온성 우모 분해균 Bacillus sp. SE-103의 분리 및 배양 조건 최적화

  • Chang, Hyung-Soo (Dept. of Food and Nutrition, Sangji University) ;
  • Choi, Il (College of Life Science and Natural Resources, Sangji University)
  • 장형수 (상지대학교 식품영양학과) ;
  • 최일 (상지대학교 동물생명자원학부)
  • Published : 2009.12.31
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Abstract

This study was carried out to investigate the possibility to utilize feather meal by bacterial strains. A bacterial strain SE-103 producing keratinolytic enzyme was isolated from the soil of the poultry slaughterhouses. It was identified as Bacillus sp. by judging from its morphological and physiological characteristics. Subsequently the optimal culture conditions for the production of keratinolytic protease by Bacillus sp. SE-103 were investigated. The composition of optimal medium was 3.0% glucose, 0.4% urea, 0.2% $NaNO_3$, and 0.15% KCl. In addition, optimal initial pH and temperature were 6.0 and $35^{\circ}C$, respectively.

본 연구는 박테리아에 의한 우모분의 이용 가능성을 조사 하기 위해 우모 keratin 분해 활성이 높은 균주를 분리한 후 keratinolytic protease 활성이 가장 우수한 효소 생산을 위한 최적 배양 조건을 조사하였다. SE-103 균주로 형태학적 생화학적 특성을 조사한 결과Bacillus sp.로 확인되어 Bacillus sp. SE-103으로 명명하였다. 최적 배지 조성은 glucose 3.0%, urea 0.4%, $NaNO_3$ 0.2%, KCl 0.15%였으며, 배양 조건은 초기 pH 6.0, 배양 온도 $35^{\circ}C$, 24시간 진탕 배양하였을 때, 효소 생산이 가장 우수하게 나타났다.

Keywords

References

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