미란형 구강편평태선과 재발성 아프타성 구내염 환자들의 비자극성 전타액내 T림프구 조절인자들의 발현 양상

Expression Pattern of T Lymphocyte Regulatory Factors in Unstimulated Whole Saliva of Erosive Oral Lichen Planus and Recurrent Aphthous Stomatitis Patients

  • 윤선학 (전남대학교 치의학연구소, 치의학전문대학원 구강내과학교실) ;
  • 고현미 (전남대학교 치의학연구소, 치의학전문대학원 구강해부학교실) ;
  • 박지일 (광주보건대학 치위생과) ;
  • 김재형 (전남대학교 치의학연구소, 치의학전문대학원 구강내과학교실)
  • Yoon, Seon-Hack (Department of Oral medicine, School of Dentistry, Dental Science Research Institute, Chonnam National University) ;
  • Ko, Hyun-Mi (Dental Science Research Institute, 2nd stage Brain Korea, School of Dentistry, Chonnam National University) ;
  • Park, Ji-Il (Department of Dental Hygiene, Gwangju Health college university) ;
  • Kim, Jae-Hyung (Department of Oral medicine, School of Dentistry, Dental Science Research Institute, Chonnam National University)
  • 발행 : 2009.12.30

초록

미란형 구강편평태선과 재발성 아프타성 구내염은 T림프구에 의해 매개되는 염증성 면역질환이다. T림프구에 영향을 주는 다양한 조절 인자들 중 CD28, CD45, CD152, CD154, CD279등의 mRNA가 미란형 구강편평태선 및 재발성 아프타성 구내염 환자들의 비자극성 전타액내에서 어떻게 발현되는지를 살펴보고, 이것의 진단학적 가치를 평가하고자 한다. 미란형 구강편평태선으로 진단받은 환자군 18명, 재발성 아프타성 구내염으로 진단받은 환자군 12명, 정상군 8명에게서 비자극성 전타액을 10분간 채득한 후, 상피세포를 분리하여 mRNA를 추출하였다. Real time PCR을 이용하여 각 군의 T림프구 조절 인자들의 mRNA 발현 양상을 비교분석하였다. 미란형 구강편평태선의 T림프구 조절인자들의 mRNA 발현 양상은 정상인과 비교 결과, CD45, CD279는 높게 측정되었고, CD154는 낮게 측정되었다. 재발성 아프타성 구내염 환자들의 T림프구 조절인자들의 mRNA 발현 양상은 정상인과 비교 결과, CD45, CD279는 높게 발현되었고, CD28, CD154는 낮게 발현되었다. 부가적으로 미란형 구강편평태선 환자군의 타액내 CD152의 발현 양상은 재발성 아프타성 구내염 환자군보다 높게 발현되었다. 미란형 구강편평태선과 재발성 아프타성 구내염 환자들의 비자극성 전타액내 T림프구 조절인자들의 mRNA 발현 양상은 각 질환의 진단에 기여할 수 있을 것으로 사료된다.

Erosive oral lichen planus (EOLP) and recurrent aphthous stomatitis (RAS) are T-cell mediated inflammatory immune disorders. It was investigated mRNA expression pattern of several regulatory factors, such as, CD28, CD45, CD152, CD154, CD279, which influence T lymphocyte in unstimulated whole saliva (UWS) of EOLP and RAS patients. It was collected unstimulated whole saliva during 10 minute in EOLP 18 people, RAS patients 12 people, healthy control 8 people. We investigated mRNA expression of T lymphocyte regulatory factors, such as, CD28, CD45, CD152, CD154, CD279, with real time reverse transcription polymerase chain reaction. In EOLP group, CD45, CD279 expressed higher and CD154 expressed lower than control. In RAS, CD45, CD270 expressed higher and CD28, CD154 expressed lower than control. In addition CD152 salivary mRNA expression of EOLP is higher than that of RAS. The above results were suggested that the mRNA expression of T lymphocyte regulatory factors in unstimulated whole saliva of EOLP and RAS contributes to diagnosis of diseases.

키워드

참고문헌

  1. Neville BW, Damm DD, Allen CM, Bouquot JE. Oral and Maxillofacial PATHOLOGY. 3rd ed., W.B. Saunders Co., USA, 2009, pp. 782-788
  2. 이윤미, 김명찬, 김종열. 구강편평태선 환자의 임상적특징에 관한 연구. 대한구강내과학회지 1996;21(1):141-151
  3. Ismail SB, Kumar SKS, Zain RB. Oral lichen planus and lichenoid reactions: etiopathogenesis, diagnosis, management and malignant transformation. J Oral Sci 2007;49:89-106 https://doi.org/10.2334/josnusd.49.89
  4. Gonzalez-Moles MA, Scully C, Gil-Montoya JA. Oral lichen planus: controversies surrounding malignant transformation. Oral Diseases 2008;14:229-243 https://doi.org/10.1111/j.1601-0825.2008.01441.x
  5. Sugerman PB, Savage NW, Walsh LJ et al. The pathogenesis of oral lichen planus. Crit Rev Oral Biol Med 2002;13(4):350-365 https://doi.org/10.1177/154411130201300405
  6. Lodi G, Scully C, Carrozzo M et al. Current controversies in oral lichen planus: report of an international consensus meeting. Part 1. Viral infection and etiopathogenesis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2005;100:40-51 https://doi.org/10.1016/j.tripleo.2004.06.077
  7. Porter SR, Scully C, Pedersen A. Recurrent aphthous stomatitis. Crit Rev Oral Biol Med 1998;9(3):306-321 https://doi.org/10.1177/10454411980090030401
  8. Jurge S, Kuffer R, Scully C, Porter SR. Mucosal diseases series, Number VI, Recurrent aphthous stomatitis. Oral diseases 2006;12:1-21 https://doi.org/10.1111/j.1601-0825.2005.01143.x
  9. Leitenberg D, Balamuth F, Bottomly K. Changes in the T cell receptor macromolecular signaling complex and membrane microdomains during T cell development and activation. Semin Immunol 2001; 13(2):129-138 https://doi.org/10.1006/smim.2000.0304
  10. Mackey MF, Barth RJ Jr, Noelle RJ. The role of CD40/CD154 interactions in the priming, differentiation, and effector function of helper and cytotoxic T cells. J Leukoc Biol 1998;63:418-428 https://doi.org/10.1002/jlb.63.4.418
  11. Sansom DM, Walker LS. The role of CD28 and cyotoxic T-lymphocyte antigen-4(CTLA-4) in regulartory T-cell biology. Immunological Reviews 2006;212:131-148 https://doi.org/10.1111/j.0105-2896.2006.00419.x
  12. Riley JL. PD-1 signaling in primary T cells. Immunological Reviews 2009;229:114-125 https://doi.org/10.1111/j.1600-065X.2009.00767.x
  13. 이종흔, 김종수. 구강생리학. 4판, 군자출판사, 1994. pp.177-210
  14. Wong DT. Salivary diagnostics powered by nanotechnologies, proteomics and genomics. JADA 2006;137:313-321 https://doi.org/10.14219/jada.archive.2006.0180
  15. Huntington ND, Tarlinton DM. CD45: direct and indirect government of immune regulation. Immunology Letters 2004;94:167-174 https://doi.org/10.1016/j.imlet.2004.05.011
  16. Holmes Nick. CD45: all is not yet crystal clear. Immunology 2006;117:145-455 https://doi.org/10.1111/j.1365-2567.2005.02265.x.
  17. Rodriguez-Nunez I, Blanco-Carrion A, Garcia AG, Rey JG. Peripheral T-cell subsets in patients with reticular and atropic-erosive oral lichen planus. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2001;91:180-188 https://doi.org/10.1067/moe.2001.110415
  18. van Kooten C, Banchereau J. CD40-CD40 ligand. J Leukoc Biol 2000 Jan;67(1):2-17 https://doi.org/10.1002/jlb.67.1.2
  19. Neppelberg E, Loro LL, Oijordsbakken G, Johannessen AC. Altered CD40 and E-cadherin expression - putative role in oral lichen planus. J Oral Pathol Med 2007;36:153-160 https://doi.org/10.1111/j.1600-0714.2007.00511.x
  20. Bour-Jordan H, Bluestone JA. Regulation the regulators: costimulatory signals controls the homeostasis and function of regulatory T cells. Immunological reviews 2009;229:41-66 https://doi.org/10.1111/j.1600-065X.2009.00775.x
  21. Zhao ZZ, Savage NW, Sugerman PB, Walsh LJ. Mast cell/T cell interactions in oral lichen planus. J Oral Pathol Med 2002;31:189-195 https://doi.org/10.1034/j.1600-0714.2002.310401.x
  22. Fife BT, Bluestone JA. Control of peripheral T-cell tolerance and autoimmunitiy via the CTLA-4 and PD-1 pathways. Immunological Reviews 2008;224:166-182 https://doi.org/10.1111/j.1600-065X.2008.00662.x
  23. Youngnak-Piboonratanakit P, Tsushima F, Otsuki N et al. The expression of B7-H1 on keratinocytes in chronic inflammatory mucocutaneous disease and its regulatory role. Immunology Letters 2004;94:215-222 https://doi.org/10.1016/j.imlet.2004.05.007