한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제37권4호
  • /
  • Pages.361-364
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  • 2009
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  • 1598-642X(pISSN)
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  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Trehalose에 의한 Rahnella aquatilis AY2000 균주가 생산하는 항효모성 물질의 활성보호

Trehalose Protects Activity of Anti-Yeast Substance Produced by Rahnella aquatilis Strain AY2000

  • 강민정 (동의대학교 자연과학대학 생명응용학과) ;
  • 이종환 (동의대학교 공과대학 생명공학과) ;
  • 이복규 (동의대학교 자연과학대학 분자생물학과) ;
  • 김광현 (동의대학교 자연과학대학 생명응용학과)
  • Kang, Min-Jung (Department of Life Science and Biotechnology, Dong-Eui University) ;
  • Lee, Jong-Hwan (Department of Biotechnology and Bioengineering, Dong-Eui University) ;
  • Lee, Bok-Kyu (Department of Molecular Biology, Dong-Eui University) ;
  • Kim, Kwang-Hyeon (Department of Life Science and Biotechnology, Dong-Eui University)
  • 발행 : 2009.12.28
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초록

AYS(Anti-yeast substance)는 열에 의해 항효모 활성이 쉽게 약화되는 물질로서 trehalose의 사용이 AYS의 열에 대한 불안정성을 어느 정도 극복할 수 있을 것인지를 조사하기 위해 trehalose가 첨가된 AYS와 trehalose가 첨가되지 않은 AYS에 대한 항효모 활성을 비교 측정하였다. 그 결과 trehalose가 첨가된 AYS는 고온($50-70^{\circ}C$)에서 단시간(2 hr) 동안 열처리한 경우에 trehalose가 함유되지 않은 AYS에 비해 그 활성이 비교적 잘 유지되었다. 따라서 trehalose를 AYS에 첨가하면 열처리 후에도 AYS의 항효모 활성을 다소 보호할 수 있다.

Rahnella aquatilis strain AY2000 produces an anti-yeast substance (AYS), however activity of the AYS tends to be decreased by heat. To investigate whether trehalose can protect AYS activity against heat, comparative studies on the AYS with and without trehalose were performed. After heat treatment at high temperatures ($50^{\circ}C-70^{\circ}C$), the AYS with trehalose had higher activity than the AYS without trehalose had. In case of AYS with trehalose (0.3-0.9M), activity of the AYS could be determined at ranging from $7.8\;{\mu}g/mL$ to $31.3\;{\mu}g/mL$ on S. cerevisiae by MTT method. Consequently, activity of the AYS after heating was well maintained by trehalose.

키워드

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