한국임상수의학회지 (Journal of Veterinary Clinics)

  • 제26권6호
  • /
  • Pages.574-577
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  • 2009
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  • 1598-298X(pISSN)
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  • 2384-0749(eISSN)
한국임상수의학회 (The Korean Society of Veterinary Clinics)
권호 표지

초음파를 통한 미니돼지의 위 운동성 평가

Evaluation of Gastric Motility with Ultrasonography in Conscious Minipigs

  • 오선경 (서울대학교 수의과대학 수의영상학과) ;
  • 장진화 (서울대학교 수의과대학 수의영상학과) ;
  • 정주현 (서울대학교 수의과대학 수의영상학과) ;
  • 최민철 (서울대학교 수의과대학 수의영상학과)
  • Oh, Sun-Kyoung (Department of Veterinary Radiology, College of Veterinary Medicine, Seoul National University) ;
  • Chang, Jin-Hwa (Department of Veterinary Radiology, College of Veterinary Medicine, Seoul National University) ;
  • Jung, Joo-Hyun (Department of Veterinary Radiology, College of Veterinary Medicine, Seoul National University) ;
  • Choi, Min-Cheol (Department of Veterinary Radiology, College of Veterinary Medicine, Seoul National University)
  • 발행 : 2009.12.31
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초록

이 연구의 목적은 초음파를 통해 미니돼지의 위 운동성을 평가하는 것이며, 초음파를 통해 측정된 위 유문동의 수축 횟수 및 위 배출시간을 이용하였다. 실험동물은 나이 5개월령, 체중 13.5~15 kg의 건강한 미니돼지 5두를 사용하였으며, 원활한 위 초음파검사를 위해 48시간 절식시켰다. 먼저 공복상태에서 3분간 유문동의 수축 횟수를 측정하였고 유문부의 단면 넓이를 측정하여 이를 기초 값으로 사용하였다. 10 ml/kg의 생리식염수를 먹인 후, 15분 간격으로 3분간 유문동의 수축 횟수를 측정하였고, 각 시간대의 유문부 단면 넓이를 측정하였다. 위 배출시간은 유문부의 단면적이 기초 값으로 돌아올 때까지의 시간으로 하였다. 공복 시 유문동의 평균 수축횟수(평균${\pm}$표준편차)는 3분간 1.60${\pm}$2.30 회였고, 생리식염수 급여 후 평균 수축횟수는 3분간 5.40${\pm}$3.62 회로 측정되었다. 위 배출시간(평균${\pm}$표준 편차)은 평균 58.06${\pm}$5.23 초를 나타내었다. 이번 실험에서 얻어진 데이터들은 미니돼지의 위 운동성 평가의 기초 데이터베이스로 사용될 수 있으며, 정상 및 여러 질환모델로서의 미니돼지에서 활용될 수 있을 것으로 사료된다.

The purpose of this study is to evaluate gastric motility of conscious minipigs with ultrasonography from contraction number of the pyloric antrum and gastric emptying time (GET). Five-month-old, clinically healthy minipigs weighing 13.5-15 kg were used for this experiment. Assessment of gastric motility was performed using saline (10 ml/kg) in all five minipigs. Contraction number of the pyloric antrum for 3 minutes and gastric emptying time were calculated. Gastric emptying time was considered to be the time at which the antral area returned to the basal value. The mean value of contraction number of the pyloric antrum before feeding was 1.60${\pm}$2.30 (mean${\pm}$SD) for 3 min and the overall mean value after saline administration was 5.40${\pm}$3.62 for 3 min. The mean value of GET by area was 58.06${\pm}$5.23 min. This data can be used as basic database for evaluation of gastric motility in minipigs as normal and various experimental models.

키워드

참고문헌

  1. Armbrust L, Hoskinson J, Lora-Michiels M, Milliken G. Gastric emptying in cats using foods varying in fiber content and kibble shapes. Vet Radiol Ultrasound 2003; 44: 339-343 https://doi.org/10.1111/j.1740-8261.2003.tb00466.x
  2. Bateman DN, Whittingham TA. Measurement of gastric emptying by real-time ultrasound. Gut 1982; 23: 524-527 https://doi.org/10.1136/gut.23.6.524
  3. Bolondi L, Bortolotti M, Santi V, Galletti T, Gaiani S, Labo G. Measurement of gastric emptying time by real-time ultrasonography. Gastroenterology 1985; 89: 752-759
  4. Burns J, Fox S. The use of a barium meal to evaluate total gastric emptying time in the dog. Vet Radiol Ultrasound 1986; 27: 169-172 https://doi.org/10.1111/j.1740-8261.1986.tb00028.x
  5. Chandler M, Guilford W, Lawoko C, Whittem T. Gastric emptying and intestinal transit times of radiopaque makers in cats fed a high-fiber diet with and without low-dose intravenous diazepam. Vet Radiol Ultrasound 1999; 40: 3-8 https://doi.org/10.1111/j.1740-8261.1999.tb01831.x
  6. Choi M, Chang J, Lee K, Nam T, Yang I, Yoon Y, Yoon J. Radiographic and ultrasonographic evaluation of gastric empting time of dogs after acupunctural stimulation. J Vet Clin 2003; 20: 49-51
  7. Choi M, Seo M, Jung J, Lee K, Yoon J, Chang D, Park RD. Evaluation of canine gastric motility with ultrasonography. J Vet Med Sci 2002; 64: 17-21 https://doi.org/10.1292/jvms.64.17
  8. Frandson RD, Wilke L, Fails AD. Anatomy of the digestive system In: Anatomy and physiology of farm animals, \6^{th} ed. Philadelphia: Lippincott Williams & Wilkins. 2003; 315-318
  9. Goggin J, Hoskinson J, Kirk C. Jewell D, Butine M. Comparison of gastric emptying times in healthy cats simultaneously evaluated with radiopaque markers and nuclear scintigraphy. Vet Radiol Ultrasound 1999; 40: 89-95 https://doi.org/10.1111/j.1740-8261.1999.tb01845.x
  10. Hornof W, Koblik P, Strombeck D, Morgan J, Hansen G. Scintigraphic evaluation of solid-phase gastric emptying in the dog. Vet Radiol Ultrasound 1989; 30: 242-248 https://doi.org/10.1111/j.1740-8261.1989.tb01794.x
  11. King P, Adam R, Pryde A, Mcdicken W, Heading R. Relationships of human antroduodenal motility and transpyloric fluid movement; non-invasive observations with real-time ultrasound. Gut 1994; 25: 1384-1391 https://doi.org/10.1136/gut.25.12.1384
  12. Kunze C, Hoskinson J, Butine M, Goggin J. Evaluation of solid phase radiolabels of dog food for gastric emptying. Vet Radiol Ultrasound 1999; 40: 169-173 https://doi.org/10.1111/j.1740-8261.1999.tb01904.x
  13. Kusunoki H, Haruma K, Tani H, Okamoto E, Summi K, Kajiyama G. Real-time ultrasonographic assessment of antroduodenal motility after ingestion of solid and liquid meals by patients with functional dyspepsia. J Gaastroenterol Hepatol 2000; 15: 1022-1027 https://doi.org/10.1046/j.1440-1746.2000.02251.x
  14. Lester N, Roberts G, Newell S, Graham J, Hartless C. Assessment of barium impregnated polyethylene spheres (BIPS) as a measure of solid-phase gastric emptying in normal dogscomparison to scintigraphy. Vet Radiol Ultrasound 1999; 40: 465-471 https://doi.org/10.1111/j.1740-8261.1999.tb00376.x
  15. Miller E, Ullrey D. The pig as a model for human nutrition. Annu Rev Nutr 1987; 7: 361-382 https://doi.org/10.1146/annurev.nu.07.070187.002045
  16. Miyabayashi T, Morgan J. Gastric emptying in the normal dog: A contrast radiographic technique. Vet Radiol Ultrasound 1984; 25: 187-191 https://doi.org/10.1111/j.1740-8261.1984.tb02143.x
  17. Ozaki K, Monnai M, Onoma M, Muramatsu H, Yogo K, Watanabe T, Oda Y, Katagiri K, Arakawa H, Itoh Z, Omura S, Takanashi H. Effects of mitemcinal (GM-611), an orally active erythromycin-derived prokinetic agent, on delayed gastric emptying and postprandial glucose in a new minipig model of diabetes. J Diabetes Complications 2008; 22: 339-347 https://doi.org/10.1016/j.jdiacomp.2007.03.005
  18. Penninck D, D’Anjou MA. Gastrointestinal tract. In: Atlas of small animal ultrasonography, Ames: Blackwell Publishing. 2008; 281-286
  19. Ricci R, Bontempo I, Corazziari E, Bella AL, Torsoli. Real time ultrasonography of the gastric antrum. Gut 1993; 34: 173-176 https://doi.org/10.1136/gut.34.2.173
  20. Steyn P, Twedt D, Toombs W. The scintigraphic evaluation of solid phase gastric emptying in normal cats. Vet Radiol Ultrasound 1995; 36: 327-331 https://doi.org/10.1111/j.1740-8261.1995.tb00270.x
  21. Steyn P, Twedt D, Toombs W. The effect of intravenous diazepam on solid phase gastric emptying in normal cats. Vet Radiol Ultrasound 1997; 38: 469-473 https://doi.org/10.1111/j.1740-8261.1997.tb00874.x
  22. Swindle M, Horneffer P, Gardner T, Gott V, Hall T, Stuart R, Baumgartner W, Borkon A, Galloway E, Reitz B. Anatomic and anesthetic considerations in experimental cardiopulmonary surgery in swine. Lab Anim Sci 1986; 36: 357-361
  23. Won W, Choi M, Lee H. Ultrasonographic observation of gastric motility in dogs. Korean J Vet Clin Med 2000; 17: 403-410