The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Mixed Infection of 16S rDNA I and V Groups of Phytoplasma in a Single Jujube Tree

  • Lee, Sang-Hun (Department of Plant Medicine, Chungbuk National University) ;
  • Han, Sang-Sub (Division of Forest Science, Chonbuk National University) ;
  • Cha, Byeong-Jin (Department of Plant Medicine, Chungbuk National University)
  • Published : 2009.03.31
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Abstract

Jujube trees infected with phytoplasma exhibit symptoms of typical witches' broom, such as yellowing, abnormally small leaves, short internodes and proliferation of shoots. A 1.2 kb fragment of the 16S rDNA from jujube phytoplasma was generated by R16F2n/R16R2 primer pair from earlier amplified P1/P7 PCR products of cloned jujube witches' broom phytoplasmas. Enzymatic restriction fragment length polymorphism (RFLP) and sequence analysis of 16S rDNA revealed that the jujube tree was infected with 16S rDNA I and V groups of phytoplasmas. Extensive comparative analyses of restriction enzyme profiles from Alu I, Hha I, Msp I, and Rsa I clearly classified the two into different phytoplasma groups. The phylogenie analyses based on 16S rDNA showed that the similarity of the two different clones was 87.5%. This is the first report of a mixed phytoplasmal infection in a single jujube tree.

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References

  1. Alma, A, Davis, R. E., Vibio, M., Danielli, A and Bosco, D.1996. Mixed infection of grapevines in northern Italy by phytoplasma including 16S rRNA RFLP subgroup 1 6SrI-B strains previously unreported in this host. Plant Dis. 80:418-421 https://doi.org/10.1094/PD-80-0418
  2. Bianco, P. A, Davis, R. E., Prince, J. P., Lee, I.-M., Gundersen, D. E., F ortusini, A and Belli, G 1993. Double and single infections by aster yellow and elm yellows MLOs in grapevines with symptoms characteristic of flavescence doree. Riv. Patol. Veg. 3:69-82
  3. Chang, B. H. and Kim, C. J. 1971. studies on the dwarf disease of mulberry tree. Serio J Korea: 17 -21
  4. Chiykowski, L. N. and Sinha, P. C. 1988. Some factors affecting the transmission of eastern peach X-mycoplasmalike organism by the leafhopper paraphlepsius irroratus. Can. J Plant Pathol. 10:85-92 https://doi.org/10.1080/07060668809501738
  5. Chiykowski, L. N. 1991. Vector-pathogen-host plant relationships of clover phyllody mycoplasmalike organism and the vetor leafhopper Paraphlepsius irroratus. Can. J Plant Pathol. 13:11-18 https://doi.org/10.1080/07060669109500958
  6. Davis, R. E. and Lee, I.-M. 1993. Cluster-specific polymerase chain reaction amplification of 16S rDNA sequences for detection and identification of mycoplasmalike organisms. Phytopathology 83:772-776 https://doi.org/10.1094/Phyto-83-772
  7. Deng, S. J. and Hiruki, C. 1991. Genetic relatedness between two nonculturable mycoplasmalike organisms revealed by nucleic acid hybridization and polymerase chain reaction. Phytopathology 81:1475-1479 https://doi.org/10.1094/Phyto-81-1475
  8. Han, S. S. and Cha, B. 2002. Genetic similarity between jujube witches' broom and muberry dwarf phytoplasmas transmitted by same vector in korea. Kor. J Plant Pathol. 18:98-101 https://doi.org/10.5423/PPJ.2002.18.2.098
  9. Kim, Y. H. 1980. Studies on mycoplasma-like organism associated with Witches' broom of Rhus javanica. Korea J Foresry 47:1-15
  10. Kim, Y. H., So, I. Y. and Han, S. S. 1994. MLO-DNA nucleotide sequence and diversity of mycoplasmal tree disease in Korea. Korea J Mycoplasmol. 5:53-59
  11. Kollar, A, Seemuller, E., Bonet, F., Saillaird, S. and Bove, J. M. 1990. Isolation of the DNA of various plant pathogenic mycoplasmalike organisms from infected plants. Phytopathology 80:3233-3237
  12. La, Y. J. and Woo, K. S. 1980. Transmission of jujube witches' broom mycoplasma by leafhopper Hishimonas sellatus Uhler. Kor. J For. 48:29-39
  13. Lee, I.-M., Gundersen., D. E. Hammond, R. W. and Davis, R. E. 1994. Use of mycoplasmalike organism (MLO) group-specific oligonucleotide primer for nested-PCR assays to detect mixed-MLO infections in a single host plant. Phytopathology 84:559-566 https://doi.org/10.1094/Phyto-84-559
  14. Lee, J. T 1988. Investigation on jujube diseases and their severities of incidence. Res. Rept. RDA (Agri. Institutional cooperation) 31:155-161
  15. Lee, J. T and Yea, M. C. 1993. Detection of mycoplasma-like organisms (MLOs) using PCR from diseased plants in Korea. Korea J Mycoplasmol. 4:36-44
  16. Liefting, L. W., Padovan, A C., Gibb, K. S., Beever, R. E., Andersen, M. T., Newcomb R. D., Beck, D. L. and Forester, R. L. S. 1998. 'Candidatus phytoplasma austraiense' is the phytoplasma associated with Australian grapevine yellows, papaya dieback and phormium yellow leaf disease. Eur. J Plant Pathol. 104:619-623 https://doi.org/10.1023/A:1008693904427
  17. McCoy, R. E., Caudwell, A, Chang, C. J., Chern, T-A, Chiykowski, L. N., Cousin, M. T, Dale, J. L., DeLeeuw, G T. N., Golino, D. A, Hackett, K. J., et al. 1989. Plant disease associated with mycoplasma-like organisms. In: The mycoplasmas, Vol. 5, ed. by R. F. Whitcomb and J. G Tully, pp. 546-640. New York Academic Press, New York, USA
  18. McCoy, R. E., Caudwell, A. and Chang, C. J. 1989. Plant diseases associated with mycoplasmalike organisms. pp. 545-560. In: The Mycoplasmas, Vol.5. Academic Press, New York
  19. Schneider, B., Seemuller, E., Smart, C. D. and Kirkpatrick, B. C. 1995. Phylogenetic classification of plant pathogenic mycoplasmal ike organism or phytoplasmas. In: Molecular and Diagnostic procedures in Mycoplasmology, Vol. 1 ed. by R. Razin and J. G Tully, pp. 369-380. Academic Press, San Diego, USA
  20. Shiomi, T. and Sugiura, M. 1984 Difference among Macrosteles orientalis-transmitted MLO, potato purple-top wilt MLO in Japan and aster yellow MLO from USA. Ann. Phytopath. Soc. Japan 50:455-460 https://doi.org/10.3186/jjphytopath.50.455
  21. Staniulis, J. B., Davis, R. E., Jomantiene, R., Kalvelyte, A. and Dally, E. L. 2000. Single and mixed phytoplasma infections in phyllody and dwarf-diseased clover plants in Lithuania. Plant Dis. 84: 1061-1066 https://doi.org/10.1094/PDIS.2000.84.10.1061

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  1. Current Status of Phytoplasmas and their Related Diseases in Korea vol.28, pp.3, 2012, https://doi.org/10.5423/PPJ.RW.04.2012.0055
  2. Mixed Infection of Two Groups (16SrI & V) of Phytoplasmas in a Single Jujube Tree in China vol.161, pp.9, 2013, https://doi.org/10.1111/jph.12101
  3. 16S rDNA sequence analysis of witches’ broom phytoplasma isolates from Chinese jujube in North China vol.45, pp.1, 2016, https://doi.org/10.1007/s13313-016-0396-5