Asian-Australasian Journal of Animal Sciences

  • 제22권2호
  • /
  • Pages.194-200
  • /
  • 2009
  • /
  • 1011-2367(pISSN)
  • /
  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
권호 표지
DOI QR코드

DOI QR Code

Effects of Donor Cell Passage, Size and Type on Development of Porcine Embryos Derived from Somatic Cell Nuclear Transfer

  • Zhang, Y.H. (Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Song, E.S. (Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Kim, E.S. (Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Cong, P.Q. (Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Lee, S.H. (Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Lee, J.W. (Department of Animal Resource Science, College of Industrial Science, Kongju National University) ;
  • Yi, Y.J. (Research Center for Transgenic Cloned Pigs, Chungnam National University) ;
  • Park, Chang-Sik (Research Center for Transgenic Cloned Pigs, Chungnam National University)
  • 투고 : 2008.06.18
  • 심사 : 2008.09.06
  • 발행 : 2009.02.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The aim of this study was to investigate the effects of donor cell passage, size and type on the development of nuclear transfer embryos. Porcine cumulus cells, fetal fibroblasts and oviductal epithelial cells from 1-2, 3-6 and 7-10 passages were used for the nuclear transfer. In the oocytes with the cumulus donor cells, fusion and cleavage rates of oocytes and cell numbers per blastocyst among the three different passage groups did not show any differences, but the rates of blastocyst formation from 1-2 and 3-6 passage groups were higher than those from 7-10 passage group. The rates of fusion, cleavage and blastocyst formation, and the cell numbers per blastocyst were higher in the embryos with the sizes of <20 and 20 ${\mu}m$ cumulus donor cells compared to the >20 ${\mu}m$ cumulus donor cell. In the oocytes with the fetal fibroblast donor cells, the rate of blastocyst formation from the 3-6 passage group was higher than from 1-2 and 7-10 passage groups. The embryos with the size of 20 $\mu{m}$ fetal fibroblast donor cell showed higher rate of blastocyst formation compared to those with <20 and >20 ${\mu}m$ donor cells. In the oocytes with the oviductal epithelial cells, the rates of blastocyst formation from 1-2 and 3-6 passage groups were higher compared to those from 7-10 passage group. The embryos with the sizes of <20 and 20 ${\mu}m$ oviductal epithelial donor cells had a higher rate of blastocyst formation compared to those with >20 ${\mu}m$ donor cell. Fusion and cleavage rates of oocytes, and cell numbers per blastocyst among the three different donor cell types from the 3-6 passage did not show any differences. However, the rate of blastocyst formation of somatic cell nuclear transfer (SCNT) embryos with the fetal fibroblast donor cell was higher than that of blastocyst formation of SCNT embryos with the cumulus and oviductal epithelial donor cells.

키워드

참고문헌

  1. Aladjem, M. I., B. T. Spike, L. W. Rodewald, T. J. Hope, M. Klemm, R. Jaenisch and G. M. Wahl. 1998, ES cells do not activate p53-dependent stress responses and undergo p53- independent apoptosis in response to DNA damage, Curr. Biol. 8:145-155 https://doi.org/10.1016/S0960-9822(98)70061-2
  2. Arat, S., S. J. Rzucidlo, J. Gibbons, K. Miyoshi and S. L. Stice. 2001, Production of transgenic bovine embryos by transfer of transfected granulosa cells into enucleated oocytes, Mol. Reprod. Dev. 60(1):20-26 https://doi.org/10.1002/mrd.1057
  3. Arat, S., J. Gibbons, S. J. Rzucidlo, D. S. Respess, M. Tumlin and S. L. Stice. 2002, In vitro development of bovine nuclear transfer embryos from transgenic clonal lines of adult and fetal fibroblast cells of the same genotype, Biol. Reprod. 66(6):1768-1774 https://doi.org/10.1095/biolreprod66.6.1768
  4. Baguisi, A., E. Behboodi, D. T. Melican, J. S. Pollock, M. M. Destrempes, C. Cammuso, J. L. Williams, S. D. Nims, C. A. Porter, P. Midura, M. J. Palacios, S. L. Ayres, R. S. Denniston, M. L. Hayes, C. A. Ziomek, H. M. Meade, R. A. Godke, W. G. Gavin, E. W. Overstr$\ddot{o}$m and Y Echelard, 1999. Production of goats by somatic cell nuclear transfer, Nat. Biotechnol. 17:456-461 https://doi.org/10.1038/8632
  5. Barnes, F. L., P. Collas, R. Powell, W. A. King, M. Westhusin and D. Shepherd. 1993, Influence of recipient oocyte cell cycle stage on DNA synthesis, nuclear envelope breakdown, chromosome constitution, and development in nuclear transplant bovine embryos, Mol. Reprod. Dev. 36(1):33-41 https://doi.org/10.1002/mrd.1080360106
  6. Betthauser, J., E. Forsberg, M. Augenstein, L. Childs, K. Eilertsen, J. Enos, T. Forsythe, P. Golueke, G. Jurgella, R. Koppang, T. Lesmeister, K. Mallon, G. Mell, P. Misica, M. Pace, M. Pfister- Genskow, N. Strelchenko, G. Voelker, S. Watt, S. Thompson and M. Bishop. 2000, Production of cloned pigs from in vitro systems, Nat. Biotechnol. 18:1055-1059 https://doi.org/10.1038/80242
  7. Bondioli, K., J. Ramsoondar, B. Williams, C. Costa and W. Fodor. 2001, Cloned pigs generated from cultured skin fibroblasts derived from a H-transferase transgenic boar, Mol. Reprod.Dev. 60(2):189-195 https://doi.org/10.1002/mrd.1076
  8. Boquest, A. C., C. G. Grupen, S. J. Harrison, S. M. McIlfatrick, R. J. Ashman, A. J. d'Apice and M. B. Nottle. 2002, Production of cloned pigs from cultured fetal fibroblast cells, Biol. Reprod. 66(5):1283-1287 https://doi.org/10.1095/biolreprod66.5.1283
  9. Campbell, K. H., P. Loi, P. J. Otaegui and I. Wilmut. 1996, Cell cycle coordination in embryo cloning by nuclear transfer, Rev. Reprod. 1:40-46 https://doi.org/10.1530/ror.0.0010040
  10. Campbell, K. H. 1999, Nuclear equivalence, nuclear transfer, and the cell cycle, Cloning 1(1):3-15 https://doi.org/10.1089/15204559950020058
  11. Chesne, P., P. G. Adenot, C. Viglietta, M. Baratte, L. Boulanger and J. P. Renard. 2002, Cloned rabbits produced by nuclear transfer from adult somatic cells, Nat. Biotechnol. 20:366-369 https://doi.org/10.1038/nbt0402-366
  12. Cibelli, J. B., S. L. Stice, P. J. Golueke, J. J. Kane, J. Jerry, C. Blackwell, F. A. Ponce de León and J. M. Robl. 1998, Cloned transgenic calves produced from nonquiescent fetal fibroblasts, Sci. 280:1256-1258 https://doi.org/10.1126/science.280.5367.1256
  13. Houdebine, L. M. 1997, Generating biological models through gene transfer to domestic animals, Vet. Res. 28:201-205
  14. Kato, Y., T. Tani, Y. Sotomaru, K. Kurokawa, J. Kato, H. Doguchi, H. Yasue and Y. Tsunoda, 1998, Eight calves cloned from somatic cells of a single adult, Sci. 282:2095-2098 https://doi.org/10.1126/science.282.5396.2095
  15. Koo, D. B., Y. K. Kang, Y. H. Choi, J. S. Park, H. N. Kim, T. Kim, K. K. Lee and Y. M. Han. 2001, Developmental potential and transgene expression of porcine nuclear transfer embryos using somatic cell, Mol. Reprod. Dev. 58:15-21 https://doi.org/10.1002/1098-2795(200101)58:1<15::AID-MRD3>3.0.CO;2-Y
  16. Kubota, C., H. Yamakuchi, J. Todoroki, K. Mizoshita, N. Tabara and M. Barber. 2000, Six cloned calves produced from adult fibroblast cells after long-term culture, Proc. Natl. Acad. Sci. USA 97:990-995 https://doi.org/10.1073/pnas.97.3.990
  17. K$\ddot{u}$hholzer, B. and R. S. Prather. 2000, Advances in livestock nuclear transfer, Proc. Soc. Exp. Biol. Med. 224(4):240-245 https://doi.org/10.1046/j.1525-1373.2000.22427.x
  18. Lee, Y. S., S. A. Ock, S. K. Cho, B. G. Jeon, T. Y. Kang, S. Balasubramanian, S. Y. Choe and G. J. Rho. 2007, Effect of donor cell types and passages on preimplantation development and apoptosis in porcine cloned embryos, Asian-Aust. J. Anim. Sci. 20(5):711-717
  19. Mather, J. P. and D. Barnes. 1998, Animal cell culture methods: methods in cell biology, London: Academic Press, pp. 31-46
  20. Onishi, A., M. Iwamoto, T. Akita, S. Mikawa, K. Takeda, T. Awata, H. Hanada and A. C. Perry. 2000, Pig cloning by microinjection of fetal fibroblast nuclei, Sci. 289:1188-1190 https://doi.org/10.1126/science.289.5482.1188
  21. Polejaeva, I. A., S. H. Chen, T. D. Vaught, R. L. Page, J. Mullins, S. Ball, Y. Dai, J. Boone, S. Walker, D. L. Ayares, A. Colman and K. H. Campbell. 2000, Cloned pigs produced by nuclear transfer from adult somatic cells, Nature 407:86-90 https://doi.org/10.1038/35024082
  22. Reik, W., W. Dean and J. Walter. 2001, Epigenetic reprogramming in mammalian development, Sci. 293:1089-1093 https://doi.org/10.1126/science.1063443
  23. Rideout, III W. M., K. Eggan and R. Jaenisch. 2001, Nuclear cloning and epigenetic reprogramming of the genome, Sci. 293:1093-1098 https://doi.org/10.1126/science.1063206
  24. Rho, S., H. Shim, W. S. Hwang and J. T. Yoon. 2000, In vitro development of green fluorescent protein (GFP) transgenic bovine embryos after nuclear transfer using different cell cycles and passages of fetal fibroblasts, Reprod. Fertil. Dev. 12(1-2):1-6
  25. Schnieke, A. E., A. J. Kind, W. A. Ritchie, K. Mycock, A. R. Scott, M. Ritchie, I. Wilmut, A. Colman and K. H. S. Campbell. 1997, Human factor IX transgenic sheep produced by transfer of nuclei from transfected fetal fibroblasts, Sci. 278:2130-2133 https://doi.org/10.1126/science.278.5346.2130
  26. Shin, T., D. Kraemer, J. Pryor, L. Liu, J. Rugila, L. Howe, S. Buck, K. Murphy, L. Lyons and M. Westhusin. 2002, A cat cloned by nuclear transplantation, Nature 415:859 https://doi.org/10.1038/nature723
  27. Tao, T., A. C. Boquest, Z. Machaty, A. L. Petersen, B. N. Day and R. S. Prather. 1999, Development of pig embryos by nuclear transfer of cultured fibroblast cells, Cloning 1(1):55-62 https://doi.org/10.1089/15204559950020094
  28. Wakayama, T., A. C. Perry, M. Zuccotti, K. R. Johnson and R. Yanagimachi. 1998, Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei, Nature394:369-374 https://doi.org/10.1038/28615
  29. Walker, S. K., K. M. Hartwich and R. F. Seamark. 1996, The production of unusually large offspring following embryo manipulation: concepts and challenges, Theriogenol 45:111-120 https://doi.org/10.1016/0093-691X(95)00360-K
  30. Wells, D. N., P. M. Misica and H. R. Tervit. 1999, Production of cloned calves following nuclear transfer with cultured adult mural granulosa cells, Biol. Reprod. 60:996-1005 https://doi.org/10.1095/biolreprod60.4.996
  31. Wilmut, I., A. E. Schnieke, J. McWhir, A. J. Kind and K. H. Campbell. 1997, Viable offspring derived from fetal and adult mammalian cells, Nature 385:810-813 https://doi.org/10.1038/385810a0
  32. Yamazaki, Y., H. Makino, K. Hamaguchi-Hamada, S. Hamada, H.Sugino, E. Kawase, T. Miyata, M. Ogawa, R. Yanagimachi and T. Yagi. 2001, Assessment of the developmental totipotency of neural cells in the cerebral cortex of mouse embryo by nuclear transfer, Proc. Natl. Acad. Sci. USA 98:14022-14026 https://doi.org/10.1073/pnas.231489398
  33. Yoo, J. G. and G. J. Rho. 2007, Effect of oocyte activation regimens on ploidy of nuclear transfer embryos reconstructed with fetal fibroblasts in rabbit, Asian-Aust. J. Anim. Sci.20(5):718-724
  34. Yoshioka, K., C. Suzuki, A. Tanaka, M. K. Anas Idris and S. Iwamura. 2002, Birth of piglets derived from porcine zygotes cultured in a chemically defined medium, Biol. Reprod.66:112-119 https://doi.org/10.1095/biolreprod66.1.112
  35. Young, L. E., K. D. Sinclair and I. Wilmut. 1998, Large offspring syndrome in cattle and sheep, Rev. Reprod. 3:155-163 https://doi.org/10.1530/ror.0.0030155

피인용 문헌

  1. Positive effects of treatment of donor cells with aphidicolin on the preimplantation development of somatic cell nuclear transfer embryos in Chinese Bama mini-pig (Sus Scrofa) vol.83, pp.2, 2012, https://doi.org/10.1111/j.1740-0929.2011.00926.x
  2. Comparative analysis of various donor cell types for somatic cell nuclear transfer and its association with apoptosis and senescence vol.9, pp.1, 2014, https://doi.org/10.3892/mmr.2013.1776
  3. Buffalo (Bubalus bubalis) SCNT embryos produced from somatic cells isolated from frozen–thawed semen: effect of trichostatin A on the in vitro and in vivo developmental potential, quality and epigenetic status vol.24, pp.04, 2016, https://doi.org/10.1017/S0967199415000520
  4. ) vol.15, pp.5, 2017, https://doi.org/10.1089/bio.2017.0060
  5. SV40 Large T Antigen Disrupts Embryogenesis of Canine and Porcine Somatic Cell Nuclear Transfer Embryo vol.19, pp.1, 2017, https://doi.org/10.1186/s12575-017-0061-6
  6. ) Embryos Produced from Somatic Cells Isolated from Milk and Ear Skin Differ in Their Developmental Competence, Epigenetic Status, and Gene Expression vol.17, pp.5, 2015, https://doi.org/10.1089/cell.2015.0027
  7. Relationship between Developmental Ability and Cell Number of Day 2 Porcine Embryos Produced by Parthenogenesis or Somatic Cell Nuclear Transfer vol.22, pp.4, 2009, https://doi.org/10.5713/ajas.2009.80362
  8. Embryonic Development and Implantation Related Gene Expression of Oocyte Reconstructed with Bovine Trophoblast Cells vol.58, pp.4, 2009, https://doi.org/10.1262/jrd.11-112h