Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Candidacidal Effects of Rev (11-20) Derived from HIV-1 Rev Protein

  • Lee, Juneyoung (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University) ;
  • Lee, Dong Hwan (Christian Academy in Japan) ;
  • Lee, Dong Gun (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University)
  • Received : 2009.08.26
  • Accepted : 2009.09.01
  • Published : 2009.10.31
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Abstract

Rev is an essential regulatory protein for HIV-1 replication. Rev (11-20) is known as the significant region regarding the function of a nuclear entry inhibitory signal (NIS) of Rev. In this study, anticandidal effects and mechanism of action of Rev (11-20) were investigated. The result exhibited that Rev (11-20) contained candidacidal activities. To understand target site(s) of Rev (11-20), the intracellular localization of the peptide was investigated. The result showed that Rev (11-20) rapidly accumulated in the fungal cell surface. The cell wall regeneration test also indicated that Rev (11-20) exerted its anticandidal activity to fungal plasma membrane rather than cell wall. The fluorescent study using 1,6-diphenyl-1,3,5-hexatriene (DPH) further confirmed the membrane-disruption mechanism(s) of Rev (11-20). The present study suggests that Rev (11-20) possesses significant potential regarding therapeutic agents for treating fungal diseases caused by Candida species in humans.

Keywords

Acknowledgement

Supported by : Rural Development Administration

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