Clinical and Experimental Pediatrics

The Korean Pediatric Society (대한소아청소년과학회)
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Factors affecting the contamination of bag urine culture in febrile children under two years

2세 미만의 열성 환아에서 소변 주머니를 이용한 소변 배양 검사의 오염률에 영향을 미치는 인자

  • Choi, Wook Hyun (Department of Pediatrics, College of Medicine, Chung-Ang University) ;
  • Lim, In Seok (Department of Pediatrics, College of Medicine, Chung-Ang University)
  • 최욱현 (중앙대학교 의과대학 소아과학교실) ;
  • 임인석 (중앙대학교 의과대학 소아과학교실)
  • Received : 2008.08.25
  • Accepted : 2008.10.31
  • Published : 2009.03.15
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Abstract

Purpose : Since children under two years with suspected urinary tract infections (UTIs) cannot control urination, urine cultures in such children are usually performed via urine bags. This method is noninvasive but has a high contamination rate. We studied the contamination rate of bag urine culture in diagnosing UTI in infants under two years and the factors responsible for contamination. Methods : We examined patients under 2 years in whom urine culture through the urine bag method yielded over 105 colonies of a single pathogen. We defined UTI by referring to the guidelines of The Korean Society of Pediatric Nephrology, 2005. We examined the factors responsible for contamination according to sex, duration of urine collection, and whether diarrhea took place with contamination rate. Results : We examined 717 patients (412 males and 305 females). The contamination rate of one bag urine culture was 37.9%. Gender was not related to the contamination rate (P>0.05). Duration of urine collection showed an association with the contamination rate. The longer the duration of collecting urine, the higher was the contamination rate. Duration of urine collection was divided into three groups: first group, <2 hours; second group, 24 hours; and third group, ${\geq}4$ hours. Contamination rates were 30.0%, 42.2%, and 43.7% for the first, second, and third groups, respectively, with statistical significance (P=0.001). Diarrhea at admission had no impact on the contamination rate (P>0.05). Conclusion : The contamination rate of urine culture in the examined patients was 37.9%. Gender and diarrhea symptoms were not responsible for contamination. In infants with a suspected UTI, urine should be collected within 2 hours through the urine bag method. If urine collection takes >2 hours, the urine bag should be resterilized and reattached to the patient.

목 적 : 요로 감염이 의심되는 2세 미만의 소아는 소변을 가릴 수 없기 때문에, 일반적으로 소변 주머니를 이용하여 소변 검사를 시행한다. 이 검사 방법은 비칩습적인 검사이지만 오염률이 높은 단점이 있다. 이에 저자들은 소변 주머니를 이용한 소변 배양 검사의 오염률에 영향을 미칠 수 있는 인자들에 대하여 알아보았다. 방 법 : 2007년 1월부터 2007년 12월까지 중앙대학교병원 소아청소년과에 내원한 환아들 중 소변 주머니를 이용한 소변 배양 검사에서 단일균이 10만개 이상 자란 환아들을 대상으로 하였다. 소변 주머니를 재부착하였던 환아들을 제외하고, 요로 감염인 환아와 오염인 환아로 분류하여, 환아의 성별, 소변 주머니를 붙인 뒤 채취에 걸린 시간, 설사 증상의 동반 유무가 오염률에 어떤 영향을 미치는지 조사하였다. 결 과 : 872명 중에서717명의 환아가 연구에 포함되었고 환아이들 중 중 남아는 412명, 여아는 305명이었다. 37.9%의 오염률을 보였고, 성별과 오염률은 통계학적으로 유의한 차이가 없었다 (P>0.05). 소변 주머니를 붙인 뒤 소변 채취에 걸린 시간이 길어질수록 오염률이 증가하였고, 2시간 이내, 4시간 이내, 4시간 이상 10시간 이내로 세 군으로 나누어 오염률을 확인하였을 때 각각 30.0%, 42.2%, 43.7%로 통계학적으로 유의하게 증가하는 것으로 나타났다(P=0.001). 내원 당시 설사 증상의 동반 여부는 배양 검사의 오염률에 영향을 미치지 않았다(P>0.05). 결 론 : 성별 및 설사 증상 유무는 소변 주머니를 이용한 소변 배양 검사의 결과에 영향을 주지 않으며, 요로 감염이 의심되는 2세 미만의 환아에게서 2시간 이내에 소변 채취시에는 정확도를 높일 수 있다. 따라서 소변 주머니를 붙인 뒤 2시간 이상 경과된 환아에게서 독성 증상이 없다면 재소독 후 재부착을 실시하는 것이 오염률을 줄이는데 도움이 될 것으로 보인다.

Keywords

Acknowledgement

Supported by : 중앙대학교

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