Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Toxicity, antibacterial and parasiticidal effects of hydrogen peroxide for israel carp (Cyprinus carpio)

과산화수소의 이스라엘잉어 (Cyprinus carpio)에 대한 독성 및 세균과 기생충 제거 효과

  • Kim, Myoung-Sug (Pathology Team, National Fisheries Research and Development Institute) ;
  • Cho, Ji-Young (Deptartment of Marine biotechnology, Soonchunhyang University) ;
  • Kim, Dae-Hee (Central Regional Inland Fisheries Research Institute) ;
  • Jeon, Hyoung-Joo (Central Regional Inland Fisheries Research Institute) ;
  • Kim, Eung-Oh (South Sea Fisheries Research Institute)
  • 김명석 (국립수산과학원 병리연구과) ;
  • 조지영 (순천향대학교 해양생명공학과) ;
  • 김대희 (국립수산과학원 중부내수면연구소) ;
  • 전형주 (국립수산과학원 중부내수면연구소) ;
  • 김응오 (국립수산과학원 남해수산연구소)
  • Published : 2009.04.01
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Abstract

To investigate the effect of hydrogen peroxide in israel carp, Cyprinus carpio, toxicity and microbial activities were determined. For hydrogen peroxide toxicity test, the median lethal concentration ($LC_{50}$) to israel carps Cyprinus carpio (average weight 0.44 g) by acute toxicity was determined after 24 hour treatment. All israel carp were alive in 24 hours treatment at 80 $\mu\ell/\ell$ concentration and $LC_{50}$ value was 148.9 $\mu\ell/\ell$. For biocidal activities of hydrogen peroxide, remove of parasite and growth inhibition of pathogenic bacteria were determined. The parasite Trichodina sp. infected on the skin and gills of israel carps (average weight 0.1 g) was completely eliminated at 40 $\mu\ell/\ell$ of hydrogen peroxide treatment for 24 hour. Most of the minimum inhibitory concentrations (MICs) against 30 fish pathogenic bacteria were less than 40 $\mu\ell/\ell$.

과산화수소의 이스라엘잉어에 대한 급성독성 시험을 실시하여 반수치사농도 ($LC_{50}$)를 구하고 기생충 제거 효과와 살균력을 조사하였다. 이스라엘잉어 (평균체중 0.44 g)는 80 $\mu\ell/\ell$에서 24시간 동안 폐사가 발생하지 않았으며 24시간 $LC_{50}$은 148.9 $\mu\ell/\ell$ 이었다. 이스라엘잉어 (평균 체중 0.1 g)에 기생된 트리코디나는 40 $\mu\ell/\ell$의 과산화수소로 1회 약욕 하였을 때 모두 제거되었다. 30종의 어병 임상균주를 사용하여 시험한 결과, 과산화수소에 대한 어병 세균의 최소억제 농도는 거의 40 $\mu\ell/\ell$보다 낮았으며 과산화수소에 의한 세균 증식 억제 효과를 확인 할 수 있었다.

Keywords

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