Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Differential Action of trans-10, cis-12 Conjugated Linoleic Acid on Adipocyte Differentiation of Ovine and 3T3-L1 Preadipocytes

  • Iga, T. (Department of Food Production Science, Faculty of Agriculture, Shinshu University) ;
  • Satoh, T. (Department of Food Production Science, Faculty of Agriculture, Shinshu University) ;
  • Yamamoto, S. (Department of Food Production Science, Faculty of Agriculture, Shinshu University) ;
  • Fukui, K. (Department of Food Production Science, Faculty of Agriculture, Shinshu University) ;
  • Song, S.H. (Department of Animal Physiology, Graduate School of Agricultural Science, Tohoku University) ;
  • Choi, K.C. (National Institute of Animal Science) ;
  • Roh, S.G. (Department of Animal Physiology, Graduate School of Agricultural Science, Tohoku University) ;
  • Sasaki, S. (Department of Food Production Science, Faculty of Agriculture, Shinshu University)
  • Received : 2008.08.26
  • Accepted : 2009.02.07
  • Published : 2009.11.01
Download PDF
⟨ Previous Next ⟩

Abstract

Trans-10, cis-12 conjugated linoleic acid (CLA) has been reported to inhibit the adipocyte differentiation of preadipocytes in non-ruminant animals (mice, rat, and human). However, the effects of trans-10, cis-12 CLA have not been clear in ruminants. The objective of this study was to investigate the effects of trans-10, cis-12 CLA on adipocyte differentiation of ovine preadipocytes. Differentiation of these preadipocytes was facilitated by treatment with trans-10, cis-12 CLA. Trans-10, cis-12 CLA increased the number and size of oil red O-stainable lipid drops as well as the levels of GPDH activity. PPAR-$\gamma{2}$ and adipophilin mRNA, adipogenic marker genes, were increased by treatment with trans-10, cis-12 CLA. This result was different from that observed with 3T3-L1 preadipocytes, a clonal cell line derived from rodents. Furthermore, trans-10, cis-12 CLA alone induced the adipocyte differentiation of ovine preadipocytes in differentiation-induction medium without troglitazone. These results suggest that CLA is an inducer and regulator in adipocyte differentiation of ovine preadipocytes, with species differences between ovine and rodent preadipocytes.

Keywords

References

  1. Blanquart, C., O. Barbier, J. C. Fruchart, B. Staels and C. Glineur. 2003. Peroxisome proliferator-activated receptors: Regulation of transcriptional activities and roles in inflammation. J. Steroid Biochem. Mol. Biol. 85:267-273 https://doi.org/10.1016/S0960-0760(03)00214-0
  2. Bonnet, M., C. Leroux, Y. Faulconnier, J. F. Hocquette, F. Bocquier, P. Martin and Y. Chilliard. 2000. Lipoprotein lipase activity and mrna are up-regulated by refeeding in adipose tissue and cardiac muscle of sheep. J. Nutr. 130:749-756
  3. Brodie, A. E., V. A. Manning, K. R. Ferguson, D. E. Jewell and C. Y. Hu. 1999. Conjugated linoleic acid inhibits differentiation of pre- and post- confluent 3t3-l1 preadipocytes but inhibits cell proliferation only in preconfluent cells. J. Nutr. 129:602-606 https://doi.org/10.1007/BF02898006
  4. Brown, J. M., M. S. Boysen, S. Chung, O. Fabiyi, R. F. Morrison, S. Mandrup and M. K. McIntosh. 2004. Conjugated linoleic acid induces human adipocyte delipidation: Autocrine/ paracrine regulation of mek/erk signaling by adipocytokines. J. Biol. Chem. 279:26735-26747 https://doi.org/10.1074/jbc.M401766200
  5. Brown, J. M. and M. K. McIntosh. 2003. Conjugated linoleic acid in humans: Regulation of adiposity and insulin sensitivity. J. Nutr. 133:3041-3046
  6. Chilliard, Y., A. Ferlay, Y. Faulconnier, M. Bonnet, J. Rouel and F. Bocquier. 2000. Adipose tissue metabolism and its role in adaptations to undernutrition in ruminants. Proc. Nutr. Soc. 59:127-134 https://doi.org/10.1017/S002966510000015X
  7. Chung, S., J. M. Brown, J. N. Provo, R. Hopkins and M. K. McIntosh. 2005a. Conjugated linoleic acid promotes human adipocyte insulin resistance through nfkappab-dependent cytokine production. J. Biol. Chem. 280:38445-38456 https://doi.org/10.1074/jbc.M508159200
  8. Chung, S., J. M. Brown, M. B. Sandberg and M. McIntosh. 2005b. Trans-10,cis-12 cla increases adipocyte lipolysis and alters lipid droplet-associated proteins: Role of mtor and erk signaling. J. Lipid Res. 46:885-895 https://doi.org/10.1194/jlr.M400476-JLR200
  9. Cook, M. E., C. C. Miller, Y. Park and M. Pariza. 1993. Immune modulation by altered nutrient metabolism: Nutritional control of immune-induced growth depression. Poult. Sci. 72:1301-1305 https://doi.org/10.3382/ps.0721301
  10. Daniel, Z. C., R. J. Wynn, A. M. Salter and P. J. Buttery. 2004. Differing effects of forage and concentrate diets on the oleic acid and conjugated linoleic acid content of sheep tissues: The role of stearoyl-coa desaturase. J. Anim. Sci. 82:747-758
  11. Dunshea, F. R., E. Ostrowska, B. Luxford, R. J. Smits, R. G. Campbell, D. N. D`Souza and B. P. Mullan. 2002. Dietary conjugated linoleic acid can decrease backfat in pigs housed under commercial conditions. Asian-Aust. J. Anim. Sci. 15: 1011-1017
  12. Feve, B. 2005. Adipogenesis: Cellular and molecular aspects. Best Pract. Res. Clin. Endocrinol. Metab. 19:483-499 https://doi.org/10.1016/j.beem.2005.07.007
  13. Granlund, L., L. K. Juvet, J. I. Pedersen and H. I. Nebb. 2003. Trans-10, cis-12-conjugated linoleic acid prevents triacylglycerol accumulation in adipocytes by acting as a ppargamma modulator. J. Lipid Res. 44:1441-1452 https://doi.org/10.1194/jlr.M300120-JLR200
  14. Griinari, J. M., B. A. Corl, S. H. Lacy, P. Y. Chouinard, K. V. Nurmela and D. E. Bauman. 2000. Conjugated linoleic acid is synthesized endogenously in lactating dairy cows by delta(9)- desaturase. J. Nutr. 130:2285-2291
  15. Ha, Y. L., J. Storkson and M. W. Pariza. 1990. Inhibition of benzo(a)pyrene-induced mouse forestomach neoplasia by conjugated dienoic derivatives of linoleic acid. Cancer Res. 50:1097-1101
  16. Hong, Y. H., Y. Nishimura, D. Hishikawa, H. Tsuzuki, H. Miyahara, C. Gotoh, K. C. Choi, D. D. Feng, C. Chen, H. G. Lee, K. Katoh, S. G. Roh and S. Sasaki. 2005. Acetate and propionate short chain fatty acids stimulate adipogenesis via GPCR43. Endocrinology 146:5092-5099 https://doi.org/10.1210/en.2005-0545
  17. House, R. L., J. P. Cassady, E. J. Eisen, M. K. McIntosh and J. Odle. 2005. Conjugated linoleic acid evokes de-lipidation through the regulation of genes controlling lipid metabolism in adipose and liver tissue. Obes. Rev. 6:247-258 https://doi.org/10.1111/j.1467-789X.2005.00198.x
  18. LaRosa, P. C., J. J. Riethoven, H. Chen, Y. Xia, Y. Zhou, M. Chen, J. Miner and M. E. Fromm. 2007. Trans-10, cis-12 conjugated linoleic acid activates the integrated stress response pathway in adipocytes. Physiol. Genomics 31:544-553 https://doi.org/10.1152/physiolgenomics.00156.2007
  19. Lee, K. N., D. Kritchevsky and M. W. Pariza. 1994. Conjugated linoleic acid and atherosclerosis in rabbits. Atherosclerosis 108:19-25 https://doi.org/10.1016/0021-9150(94)90034-5
  20. Liew, C., H. A. Schut, S. F. Chin, M. W. Pariza and R. H. Dashwood. 1995. Protection of conjugated linoleic acids against 2-amino-3- methylimidazo[4,5-f]quinoline-induced colon carcinogenesis in the f344 rat: A study of inhibitory mechanisms. Carcinogenesis 16:3037-3043 https://doi.org/10.1093/carcin/16.12.3037
  21. MacDonald, H. B. 2000. Conjugated linoleic acid and disease prevention: A review of current knowledge. J. Am. Coll. Nutr. 19:111S-118S
  22. Miller, C. C., Y. Park, M. W. Pariza and M. E. Cook. 1994. Feeding conjugated linoleic acid to animals partially overcomes catabolic responses due to endotoxin injection. Biochem. Biophys. Res. Commun. 198:1107-1112 https://doi.org/10.1006/bbrc.1994.1157
  23. Mir, P. S., T. A. McAllister, S. Scott, J. Aalhus, V. Baron, D. McCartney, E. Charmley, L. Goonewardene, J. Basarab, E. Okine, R. J. Weselake and Z. Mir. 2004. Conjugated linoleic acid-enriched beef production. Am. J. Clin. Nutr. 79:1207S-1211S
  24. Moya-Camarena, S. Y., J. P. Van den Heuvel and M. A. Belury. 1999. Conjugated linoleic acid activates peroxisome proliferator-activated receptor alpha and beta subtypes but does not induce hepatic peroxisome proliferation in spraguedawley rats. Biochim. Biophys. Acta. 1436:331-342 https://doi.org/10.1016/S0005-2760(98)00121-0
  25. Perez-Matute, P., A. Marti, J. A. Martinez, M. P. Fernandez-Otero, K. L. Stanhope, P. J. Havel and M. J. Moreno-Aliaga. 2007. Conjugated linoleic acid inhibits glucose metabolism, leptin and adiponectin secretion in primary cultured rat adipocytes. Mol. Cell. Endocrinol. 268:50-58 https://doi.org/10.1016/j.mce.2007.01.013
  26. Poirier, H., I. Niot, L. Clement, M. Guerre-Millo and P. Besnard. 2005. Development of conjugated linoleic acid (cla)-mediated lipoatrophic syndrome in the mouse. Biochimie. 87:73-79 https://doi.org/10.1016/j.biochi.2004.11.006
  27. Ringseis, R., A. Muller, C. Herter, S. Gahler, H. Steinhart and K. Eder. 2006. Cla isomers inhibit tnfalpha-induced eicosanoid release from human vascular smooth muscle cells via a ppargamma ligand-like action. Biochim. Biophys. Acta 1760:290-300 https://doi.org/10.1016/j.bbagen.2005.12.002
  28. Roh, S. G., D. Hishikawa, Y. H. Hong and S. Sasaki. 2006. Control of adipogenesis in ruminants. Anim. Sci. J. 77:472-477 https://doi.org/10.1111/j.1740-0929.2006.00374.x
  29. Roh, S. G., Y. H. Hong, D. Hishikawa, H. Tsuzuki, H. Miyahara, Y. Nishimura, C. Goto, Y. Suzuki, K. C. Choi, H. G. Lee and S. Sasaki. 2005. Inhibition of growth hormone secretagogue receptor antagonist, [d-lys-3]-GHRP-6, in adipogenesis of ovine and rat adipocytes. Anim. Sci. J. 76:381 https://doi.org/10.1111/j.1740-0929.2005.00279.x
  30. Rosen, E. D. and B. M. Spiegelman. 2000. Molecular regulation of adipogenesis. Annu. Rev. Cell Dev. Biol. 16:145-171 https://doi.org/10.1146/annurev.cellbio.16.1.145
  31. Sasaki, S. 2002. Mechanism of insulin action on glucose metabolism in ruminants. Anim. Sci. J. 73:423-433 https://doi.org/10.1046/j.1344-3941.2002.00059.x
  32. Sisk, M. B., D. B. Hausman, R. J. Martin and M. J. Azain. 2001. Dietary conjugated linoleic acid reduces adiposity in lean but not obese zucker rats. J. Nutr. 131:1668-1674
  33. Takahashi, K., K. Kawamata and Y. Akiba. 2007. Effect of a mixture of conjugated linoleic acid (CLA) isomers on t cell subpopulation and responsiveness to mitogen in splenocytes of male broiler chicks. Asian-Aust. J. Anim. Sci. 20:954-961
  34. Valet, P., G. Tavernier, I. Castan-Laurell, J. S. Saulnier-Blache and D. Langin. 2002. Understanding adipose tissue development from transgenic animal models. J. Lipid Res. 43:835-860
  35. van Bilsen, M., G. J. van der Vusse, A. J. Gilde, M. Lindhout and K. A. van der Lee. 2002. Peroxisome proliferator-activated receptors: Lipid binding proteins controling gene expression. Mol. Cell. Biochem. 239:131-138 https://doi.org/10.1023/A:1020553428294
  36. Wang, Y., and P. J. Jones. 2004. Dietary conjugated linoleic acid and body composition. Am. J. Clin. Nutr. 79:1153S-1158S
  37. Wolins, N. E., B. K. Quaynor, J. R. Skinner, M. J. Schoenfish, A. Tzekov and P. E. Bickel. 2005. S3-12, adipophilin, and tip47 package lipid in adipocytes. J. Biol. Chem. 280:19146-19155 https://doi.org/10.1074/jbc.M500978200
  38. Zhou, J. 2008. Effect of dietary conjugated linoleic acid (cla) on abdominal fat deposition in yellow-feather broiler chickens and its possible mechanism. Asian-Aust. J. Anim. Sci. 21: 1760-1765

Cited by

  1. Natural antioxidants in milk and dairy products vol.70, pp.2, 2017, https://doi.org/10.1111/1471-0307.12359