Asian-Australasian Journal of Animal Sciences

  • 제22권11호
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  • Pages.1566-1573
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  • 2009
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Differential Action of trans-10, cis-12 Conjugated Linoleic Acid on Adipocyte Differentiation of Ovine and 3T3-L1 Preadipocytes

  • Iga, T. (Department of Food Production Science, Faculty of Agriculture, Shinshu University) ;
  • Satoh, T. (Department of Food Production Science, Faculty of Agriculture, Shinshu University) ;
  • Yamamoto, S. (Department of Food Production Science, Faculty of Agriculture, Shinshu University) ;
  • Fukui, K. (Department of Food Production Science, Faculty of Agriculture, Shinshu University) ;
  • Song, S.H. (Department of Animal Physiology, Graduate School of Agricultural Science, Tohoku University) ;
  • Choi, K.C. (National Institute of Animal Science) ;
  • Roh, S.G. (Department of Animal Physiology, Graduate School of Agricultural Science, Tohoku University) ;
  • Sasaki, S. (Department of Food Production Science, Faculty of Agriculture, Shinshu University)
  • 투고 : 2008.08.26
  • 심사 : 2009.02.07
  • 발행 : 2009.11.01
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초록

Trans-10, cis-12 conjugated linoleic acid (CLA) has been reported to inhibit the adipocyte differentiation of preadipocytes in non-ruminant animals (mice, rat, and human). However, the effects of trans-10, cis-12 CLA have not been clear in ruminants. The objective of this study was to investigate the effects of trans-10, cis-12 CLA on adipocyte differentiation of ovine preadipocytes. Differentiation of these preadipocytes was facilitated by treatment with trans-10, cis-12 CLA. Trans-10, cis-12 CLA increased the number and size of oil red O-stainable lipid drops as well as the levels of GPDH activity. PPAR-$\gamma{2}$ and adipophilin mRNA, adipogenic marker genes, were increased by treatment with trans-10, cis-12 CLA. This result was different from that observed with 3T3-L1 preadipocytes, a clonal cell line derived from rodents. Furthermore, trans-10, cis-12 CLA alone induced the adipocyte differentiation of ovine preadipocytes in differentiation-induction medium without troglitazone. These results suggest that CLA is an inducer and regulator in adipocyte differentiation of ovine preadipocytes, with species differences between ovine and rodent preadipocytes.

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