Molecules and Cells

  • 제27권6호
  • /
  • Pages.657-665
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  • 2009
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  • 1016-8478(pISSN)
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  • 0219-1032(eISSN)
한국분자세포생물학회 (Korean Society for Molecular and Cellular Biology)
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Thermodynamic Analyses of the Constitutive Splicing Pathway for Ovomucoid Pre-mRNA

  • Ro-Choi, Tae Suk (Medical Research Center for Cancer Molecular Therapy, Dong-A University College of Medicine) ;
  • Choi, Yong Chun (Medical Research Center for Cancer Molecular Therapy, Dong-A University College of Medicine)
  • 투고 : 2009.01.28
  • 심사 : 2009.04.22
  • 발행 : 2009.06.30
⟨ 이전 논문 다음 논문 ⟩

초록

The ovomucoid pre-mRNA has been folded into mini-hairpins adaptable for the RNA recognition motif (RRM) protein binding. The number of mini-hairpins were 372 for pre-mRNA and 83-86 for mature mRNA. The spatial arrangements are, in average, 16 nucleotides per mini-hairpin which includes 7 nt in the stem, 5.6 nt in the loop and 3.7 nt in the inter-hairpin spacer. The constitutive splicing system of ovomucoid-pre-mRNA is characterized by preferred order of intron removal of 5/6 > 7/4 > 2/1 > 3. The 5' splice sites (5'SS), branch point sequences (BPS) and 3' splice sites (3'SS) were identified and free energies involved have been estimated in 7 splice sites. Thermodynamic barriers for splice sites from the least (|lowest| -Kcal) were 5, 4, 7, 6, 2, 1, and 3; i.e., -18.7 Kcal, -20.2 Kcal, -21.0 Kcal, -24.0 Kcal, - 25.4 Kcal, -26.4 Kcal and -28.2 Kcal respectively. These are parallel to the kinetic data of splicing order reported in the literature. As a result, the preferred order of intron removals can be described by a consideration of free energy changes involved in the spliceosomal assembly pathway. This finding is consistent with the validity of hnRNP formation mechanisms in previous reports.

키워드

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피인용 문헌

  1. Chemical Approaches for Structure and Function of RNA in Postgenomic Era vol.2012, pp.None, 2009, https://doi.org/10.1155/2012/369058