Korean Journal of Plant Taxonomy (식물분류학회지)

The Korean Society of Plant Taxonomists (한국식물분류학회)
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Cytogenetic Study of Maackia amurensis Rupr. & Maxim. and M. fauriei (Levl.) Takeda Using Karyotyping Analysis and the FISH Technique

핵형분석과 FISH 기술을 이용한 솔비나무와 다릅나무의 세포유전학적 연구

  • Kim, Soo-Young (Biological Resources Coordination Division, National Institute of Biological Resources) ;
  • Kim, Chan-Soo (Warm-Temperate Forest Research Center, Korea Forest Research Institute)
  • 김수영 (국립생물자원관 생물자원총괄과) ;
  • 김찬수 (국립산림과학원 난대산림연구소)
  • Published : 2009.09.30
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Abstract

Chromosome analysis using karyotyping and bicolor FISH were carried out for two Maackia species (M. fauriei and M. amurensis) found in Korea. The somatic metaphase chromosome number was 2n = 2x = 18 in both, and the size of these chromosomes ranged from 3.58 to $5.82{\mu}m$. The chromosome complements consisted of two pairs of metacentric (chromosomes 1 and 7), four pairs of submetacentrics (chromosomes 4, 6, 8 and 9) and three pairs of subtelocentrics (chromosomes 2, 3 and 5) in M. fauriei but, chromosomes 4 (subtelocentric) and 7 (submetacentric) of M. amurensis have different morphology. Using bicolor FISH, a pair of 45S rDNA loci were observed for both M. fauriei and M. amurensis, but the number and site of the 5S rDNA signal were different in the two species. M. fauriei has two pairs of 5S signals on chromosomes 7 and 8 but, M. amurensis has four paris on chromosomes 3, 4, 7 and 7. Hence, the 5S rDNA is a useful FISH for Maackia species.

국내에 자생하는 Maackia속 2종(솔비나무, 다릅나무)의 염색체수 및 핵형을 분석하고 5S와 45S rDNAs를 이용한 bicolor FISH를 수행하였다. 솔비나무와 다릅나무의 체세포 염색체수는 동일하게 2n = 2x = 18로 관찰되었고, 염색체의 길이는 $3.58{\sim}5.82{\mu}m$이였다. 솔비나무의 염색체 조성은 2쌍의 중부 염색체(염색체 1과 7번), 4쌍의 차중부 염색체(염색체 4, 6, 8, 9번), 그리고 3쌍의 차단부 염색체(염색체 2, 3, 5번)로 확인되었다. 다릅나무의 염색체는 4번이 차단부 염색체, 7번이 차중부 염색체로 솔비나무와 차이를 보였으나 다른 염색체의 동원체 위치는 유사하게 관찰되었다. 5S와 45S rDNA를 이용한 FISH 결과, 45S rDNA 유전자는 솔비나무와 다릅나무에서 각각 1쌍으로 관찰되었고 2번 염색체의 2차 협착 부위에서 확인되었다. 5S rDNA유전자를 이용한 물리지도 작성에서는 두 종 사이를 구별할 수 있는 결과를 확인할 수 있었다. 솔비나무의 경우 염색체 7번과 8번의 동원체 부위에서 2쌍이 각각 관찰되었고, 다릅나무에서는 염색체 7번과 8번뿐만 아니라 3번과 4번 염색체에서도 관찰되어 모두 4쌍으로 확인되었다. 따라서, 5S rDNA유전자를 이용한 FISH방법을 통해 세포학적으로 두 종을 구분할 수 있었다.

Keywords

Acknowledgement

Supported by : 학술진흥재단

References

  1. Boufford, D. E., H. Ohashi, T. C. Huang, C. F. Shieh, J. L. Tsai, K .C. Yang, C. I. Peng, C. S. Kuoh and A. Hsiao. 2003. In Flora of Taiwan (6). Editorial Committee of the Flora of Taiwan (2n ed.). National Taiwan Univ. Taiwan. Pp. 15-139
  2. Castilho, A. and J. S. Heslop-Harrison. 1995. Physical mapping of 5S and 18S-25S rDNA and repetitive DNA sequences in Aegilops umbellulata. Genome 38: 91-96 https://doi.org/10.1139/g95-011
  3. Choi, B. H. 2007. Fabaceae Lindl. In The Genera of Vascular Plants of Korea. Editorial Committee of the Flora of Korea. Academy Publishing Co. Seoul. Pp. 585-622
  4. Fukui, K., N. Ohmido and G. S. Khush. 1994. Variability in rDNA loci in the genus Oryza detected through fluorescence in situ hybridization. Theor. Appl. Genet. 87: 893-899
  5. Goldblatt, P. 1981. Chromosome Numbers in Legumes II. Annals of the Missouuri Botanical Garden 68: 551-557 https://doi.org/10.2307/2398889
  6. Goldblatt P. and G. Davides. 1977. Chromosome Numbers in Legumes. Annals of the Missouri Botanical Garden 64: 121-128 https://doi.org/10.2307/2395237
  7. Kim, S. Y., H. W. Choi, D. H. Koo, C. S. Kim and J. W. Bang. 2005. Karyotype analysis and physical mapping of rDNAs using McFISH in Jeffersonia dubia Benth. Korean J. Med. Crop Sci. 13: 48-51
  8. Kim, S. Y., H. W. Choi and J. W. Bang. 2004. Physical mapping of rDNAs using McFISH in Anemarrhena asphodeloides Bunge. Korean J. Med. Crop Sci. 12: 515-518
  9. Kim, S. Y., H. W. Choi, D. H. Koo, W. K. Lee, J. K. Lee and J. W. Bang. 2006a. Characterization of eight Rumex species by FISH (fluorescence in situ hybridization) and 5S rDNA spacer sequences. Korean J. Genetics 28: 243-251
  10. Kim, S. Y., H. W. Choi, C. S. Kim, J. S. Sung, J. K. Lee and J. W. Bang. 2006b. Cytogenetic analysis of Astragalus species. Korean J. Med. Crop Sci. 14: 250-254
  11. Kim, S. Y., J. W. Bang and J. K. Lee. 2006c. Cytogenetic analysis using mitosis, meiosis chromosomes and bicolor fluorescence in situ hybridization of Bupleurum latissimum Nakai. Korean J. Med. Crop Sci. 14: 354-359
  12. Kolbek, J., M. Srutek and E. Box. 2003. Forest Vegetation of Northeast Asia. Kluwer Academic Publishers, Dordrecht
  13. Koo, D. H., S. Y. Kim, K. W. Bang, N. S. Seong and J. W. Bang. 2003. Cytogenetic analysis of Angelica plants using Feulgen staining and multicolor fluorescence in situ hybridization. Korean J. Plant Biotechnology 30: 123-127 https://doi.org/10.5010/JPB.2003.30.2.123
  14. Lee, J. K. and S. Y. Kim. 2007. Chromosome Index of Plants in Korea 2007. Korea Research Institute of Bioscience and Biotechnology
  15. Lee, J. K. and S. Y. Kim. 2008. Chromosomes of Endemic Plants in Korea 2008. Korea Research Institute of Bioscience and Biotechnology
  16. Lee, T. B. 2003. Colored Flora of Korea (I). Hyangmunsa, Seoul (in Korean)
  17. Lee, W. T. 1996a. Lineamenta Florae Koreae. Academy Publishing Co. Seoul (in Korean)
  18. Lee, W. T. 1996b. Standard Illustration of Korean Plants. Academy Publishing Co. Seoul (in Korean)
  19. Lee, W. K., H. W. Choi, D. H. Koo, S. Y. Kim and J. W. Bang. 2005. Molecular cytogenetics of five Pulsatilla species to the 5S, 45S rDNA genes by fluorescence in situ hybridization. Korean J. Genetics 27: 179-185
  20. Leitch, I. J. and J. S. Heslop-Harrison. 1992. Physical mapping of the 18S-5.8S-26S rDNA genes in barley by in situ hybridization. Genome 53: 1013-1018
  21. Levan, A., K. Frekga and A. Sandberg. 1964. Nomenclature for centromeric position in chromosomes. Hereditas 52: 201-220 https://doi.org/10.1111/j.1601-5223.1964.tb01953.x
  22. Levings, C. K. 2006. A Monograph of the Genus Maackia. Master dissertation of Miami Univ
  23. Li, P., L. Fu and T. Hong. 1998. Higher Plants of China. Qingtao Publishing House
  24. Maluszynska, J. and J. S. Heslop-Harrison. 1991. Localization of tandemly repeated DNA sequences in Arabidopsis thaliana. Plant J. 1: 159-166 https://doi.org/10.1111/j.1365-313X.1991.00159.x
  25. Maluszynska, J. and J. S. Heslop-Harrison. 1993. Physical mapping of rDNA in Braddica species. Genome 36: 774-781 https://doi.org/10.1139/g93-102
  26. Tropicos.org. 2009. Missouri Botanical Garden,
  27. Mukai, Y., T. R. Endo and B. S. Gell. 1991. Physical mapping of the 18S rRNA multigene family in common wheat: identification of a new locus. Chromosoma 100: 71-78 https://doi.org/10.1007/BF00418239
  28. Ohashi, H. 2001. Maackia Rupr. & Maxim. In Flora of Japan (IIb): Angiospermae; Dicotyledonieae; Archichlamideae (b). Iwatsuki, K., D.E. Boufford & H. Ohba. (Eds.). Kodansha Ltd., Tokyo. Pp. 221-222
  29. Probatova N. S. and A. P. Sokolovskaya. 1981. Kariogicheskoe issledovanie sosudistykh rastenij ostrovov Dal'nevostocnogo gosudarstvennogo morskogo sapovednika. Sb. Cvetkaye Rastenija Ostrovov Dalnevostochnogo Morskogo Sapovendnika. Pp. 92-114
  30. Ruprecht, F. J. and C. J. Maximowicz. 1856. Maackia. Bull. Cl. Phys.-Math. Acad. Imp. Sci. Saint-Petersbourg 15: 128-143
  31. Takeda, H. 1913. Maackia. Notes from the Royal Botanic Garden, Edinburgh. 8: 101
  32. Volkova, S. A., D. D. Basargin and P. G. Gorovoy. 1994. Chromosome numbers in representatives of some families of the flora of Russian Far East. Botani${\check{c}}$esij ${\check{Z}}$urnal (Moscow & Leningrad) 79: 122-123
  33. Yeh, M. S., H. Yuasa and F. Maekawa. 1986. Chromosome numbers in the Leguminosae. Sci. Rep. Res. Inst. Evol. Biol. 3: 57-71