Clinical and Experimental Pediatrics

The Korean Pediatric Society (대한소아청소년과학회)
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Molecular identification and clinical features of enteroviral infection in children of central Korea: An overview of enteroviral epidemiology between spring 2005 and autumn 2006

2005년 봄부터 2006년 가을까지 국내 중부지역의 enterovirus 감염의 분자유전학 조사와 임상양상

  • Roh, Eui Jung (Department of Pediatrics, College of Medicine, Konyang University Daejeon) ;
  • Jin, Yong Man (Department of Pediatrics, College of Medicine, Dankook University) ;
  • Chung, Eun Hee (Department of Pediatrics, College of Medicine, Dankook University) ;
  • Chang, Young Pyo (Department of Pediatrics, College of Medicine, Dankook University) ;
  • Park, Woo Sung (Department of Pediatrics, College of Medicine, Dankook University) ;
  • Park, Kwisaung (Chungcheongnam-Do Health and Environment Research Institute) ;
  • Jee, Young Mee (Division of Enteric and Hepatitis Viruses, Department of Virology, National Institute of Health, Korea Center for Disease Control and Prevention)
  • 노의정 (건양대학교 의과대학 소아청소년과학교실) ;
  • 진용만 (단국대학교 의과대학 소아청소년과학교실) ;
  • 정은희 (단국대학교 의과대학 소아청소년과학교실) ;
  • 장영표 (단국대학교 의과대학 소아청소년과학교실) ;
  • 박우성 (단국대학교 의과대학 소아청소년과학교실) ;
  • 박귀성 (충청남도 보건환경연구회) ;
  • 지영미 (질병관리본부 간염, 장바이러스과)
  • Received : 2009.05.11
  • Accepted : 2009.10.05
  • Published : 2009.11.15
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Abstract

Purpose:Enteroviruses (EVs) are commonly known to cause infection, especially in infants and children. This report presents an overview of enterovirus epidemiology in central Korea. Methods:From the spring of 2005 to the autumn of 2006, we collected the cerebrospinal fluid (CSF) and stool samples from the pediatric patients with a febrile illness or suspected meningitis who were admitted to hospitals in central Korea. In order to test for EVs, cell lines were derived from pretreated susceptible specimen, and the cytopathic effects were observed. Seminested real time-polymerase chain reaction (RT-PCR) and direct sequencing were performed for genotypic and phylogenetic analyses. Results:Of the 305 patients examined, 51 (16.7%) tested positive for EV. Of these 51 patients, 44 showed the following serotypes: Echovirus (ECV) 18 (18 cases, 35.2%), Coxsackievirus B (CVB) 5 (13 cases, 25.4%), ECV25 (5 cases, 9.8%), ECV9 (4 cases, 7.8%), ECV5 (3 cases, 5.8%), and EV74 (1 case, 1.9%). In 2005, between June and August, ECV18 and CVB5 were mostly responsible for the enteroviral infections among the patients in central Korea. In 2006, between July and August, ECV25 was mostly the cause of enteroviral infection. Conclusions:There is a need for continuous surveillance of enteroviral infection and its clinical manifestations, particularly for EV74, which was first identified in Korea.

목 적:Enteroviruse (EV)는 영아와 소아에서 가장 흔한 감염의 원인으로 경한 발열성 질환에서 뇌수막염이나 치명적인 심근염까지 다양한 질환을 일으키는 원인이다. 본 연구에서는 국내 중부지역의 EV 유병율에 대해 알아보고자 하였다. 방 법:2005년 4월부터 2006년 10월까지 국내 중부지역에서 발열성 질환이나 뇌수막염이 의심되어 입원한 소아에서 얻은 검체를 모아 RT-PCR을 시행하여 EV를 확인하였고, genotype과 phylogenetic 분석을 위해 direct sequencing을 시행하였다. 결 과:뇌척수액과 대변검체에서 PCR을 시행한 305명의 환아 중 51명(16.7%)에서 EV가 양성을 보였다. 그중 다음의 serotype이 44명에서 확인되었다: Echovirus (ECV) 18 (18 cases, 35.2%), Coxsackievirus (CVB) 5 (13 cases, 25.4%), ECV25 (5 cases, 9.8%), ECV9 (4 cases, 7.8%), ECV5 (3 cases, 5.8%), EV74 (1 case, 1.9%). 2005년 6월과 8월 사이에는 ECV18과 CVB5가 가장 많이 검출되었다. 2006년 7월과 8월 사이에는 ECV25가 가장 많이 검출되었다. 결 론:지속적인 enterovirus 감염의 감시가 필요하며 임상적인 의의가 밝혀져야 할 것이다. 특히 EV74가 처음으로 국내에서 발견되었기에 이를 보고하는 바이다.

Keywords

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