Asian-Australasian Journal of Animal Sciences

  • 제22권2호
  • /
  • Pages.155-161
  • /
  • 2009
  • /
  • 1011-2367(pISSN)
  • /
  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
권호 표지
DOI QR코드

DOI QR Code

Cloning of Chicken Microsomal Glutathione S-transferase 1 Gene (MGST1) and Identification of Its Different Splice Variants

  • Wang, X.-T. (State Key Laboratory of Agrobiotechnology and the Key laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University) ;
  • Zhang, H. (State Key Laboratory of Agrobiotechnology and the Key laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University) ;
  • Zhao, C.-J. (State Key Laboratory of Agrobiotechnology and the Key laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University) ;
  • Li, J.-Y. (State Key Laboratory of Agrobiotechnology and the Key laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University) ;
  • Xu, G.-Y. (State Key Laboratory of Agrobiotechnology and the Key laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University) ;
  • Lian, L.-S. (College of Animal Science and Technology, Yunnan Agricultural University) ;
  • Wu, C.-X. (State Key Laboratory of Agrobiotechnology and the Key laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University) ;
  • Deng, Xuemei (State Key Laboratory of Agrobiotechnology and the Key laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University)
  • 투고 : 2008.03.16
  • 심사 : 2008.08.04
  • 발행 : 2009.02.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Mammal microsomal glutathione transferase 1 (MGST1) can conjugate many toxic or carcinogenic substances and depress oxidative stress. In this study, Chicken MGST1 and its variants were cloned for the first time and were composed of 956 or 944 nucleotides. The 12 nt deletion in the exon 2 did not alter the GT-AG rule and the ORFs for the two MGST1 variants were the same, which both comprised 465 nucletides and encoded a peptide with 155 amino acids. It was found that the two different splice variants identified using RT-PCR expressed in all three organs investigated of Dwarf Brown Chicken, namely liver, spleen and shell gland. Moreover, the expression level of MGST1 mRNA in the liver of Dwarf Brown chickens was the highest (p<0.01), and there were no significant differences between the spleen and the shell gland. These results provide a base for studying the biological function of Chicken MGST1.

키워드

참고문헌

  1. DeJong, J., H. Morgenstern, H. Jornvall, J. W. Depierre and C. P. Tu. 1998, Gene expression of rat and human microsomal glutathione S-transferases, J. Biol. Chem. 263:8430-8436
  2. Iida, A., S. Saito, A. Sekine, S. Harigae, S. Osawa, C. Mishima, K. Kondo, Y. Kitamura and Y, Nakamura. 2001. Catalog of 46 single-nucleotide polymorphisms (SNPs) in the microsomal glutathione S-transferase 1 (MGST1) gene, J. Hum. Genet. 46:590-594 https://doi.org/10.1007/s100380170026
  3. Jiang, Z. Y., S. Q. Jiang, Y. C. Lin, X. Y. Ma, P. B. Xi, T. Cao and X. Q. Wang. 2008, Effect of genistein on antioxidative defence system and membrane fluidity in chick skeletal muscle cells, Asian-Aust. J. Anim. Sci. 21:1220-1225
  4. Kumar, S. K., Tamura and M. Nei. 2004, MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment, Brief. Bioinform. 5:150-163 https://doi.org/10.1186/1471-2105-5-150
  5. Kelner, M. J., R. D. Bagnell, M. A. Montoya, L. A. Estes, L. Forsberg and R, Morgenstern. 2004, Structural organization of the murine microsomal glutathione S-transferase gene (MGST1) from the 129/SvJ strain: identification of the promoter region and a comprehensive examination of tissue expression, Biochim. Biophys. Acta. 1678:163-169 https://doi.org/10.1016/j.bbaexp.2004.03.001
  6. Kelner, M. J., R. D. Bagnell, M. A. Montoya, L. A. Estes, L. Forsberg and R, Morgenstern, 2000. Structural organization of the microsomal glutathione S-transferase gene (MGST1) on chromosome 12p13.1-13.2, J. Biol. Chem, 275:13000-13006 https://doi.org/10.1074/jbc.275.17.13000
  7. Ma, D., Y. Liu, S. Liu, Q. Li and A. Shan. 2007, Influence of Ligustrum lucidum and Schisandra chinensis fruits on antioxidative metabolism and immunological parameters of layer chicks, Asian-Aust. J. Anim. Sci. 20:1438-1443
  8. Maeda, A., J. W. Crabb and K. Palczewski. 2005, Microsomal glutathione S-transferase 1 in the retinal pigment epithelium: Protection against oxidative stress and a potential role in aging. Biochem, 44:480-489 https://doi.org/10.1021/bi048016f
  9. Matzura, O. and A. Wennborg. 1996. RNAdraw: an integrated program for RNA secondary structure calculation and analysis under 32-bit microsoft windows. Comput. Appli. Biosci. 12: 247-249
  10. Mosialou, E., G. Ekstrom, A. E. Adang and R. Morgenstern. 1993, Evidence that rat liver microsomal glutathione transferase is responsible for glutathione-dependent protection against lipid peroxidation. Biochem, Pharmacol. 45:1645-1651 https://doi.org/10.1016/0006-2952(93)90305-G
  11. Rousseau, P., M. Le Discorde, G. Mouillot, C. Marcou, E. D. Carosella and P. Moreau. 2003, The 14 bp deletion-insertion polymorphism in the 3' UT region of the HLA-G gene influences HLA-G mRNA stability, Hum. Immunol. 64:1005- 1010 https://doi.org/10.1016/j.humimm.2003.08.347
  12. Sambrook, J. and D. W. Russel. 2001, Molecular cloning: a laboratory manual. 3rd ed, Cold Spr. Harbor Lab. Press, NewYork
  13. Schmidt-Krey, I., K. Mitsuoka, T. Hirai, K. Murata, Y. Cheng, Y. Fujiyoshi, R, Morgenstern and H. Hebert. 2000. The threedimensional map of microsomal glutathione transferase 1 at 6Å resolution, EMBO J. 19:6311-6316 https://doi.org/10.1093/emboj/19.23.6311
  14. Wang, L. and Z. R. Xu. 2006, Effects of arsenic (AsIII) on lipid peroxidation, glutathione content and antioxidant enzymes in growing pigs, Asian-Aust. J. Anim. Sci 19:727-733