Applied Chemistry for Engineering (공업화학)

The Korean Society of Industrial and Engineering Chemistry (한국공업화학회)
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Production of Bio-ethanol from Agar using Saccharomyces cerevisiae

Saccharomyces cerevisiae 에 의한 Agar로부터 바이오 에탄올 생산

  • Lee, Sung-Mok (Department of Bioscience and Biotechnology, College of Engineering, Silla University) ;
  • Yu, Byung Jo (Bio & Health Group, Emerging Center, Samsung Advanced Institute of Technology) ;
  • Kim, Young Min (Department of Bioscience and Biotechnology, College of Engineering, Silla University) ;
  • Choi, Soo-Jeong (Department of Bioscience and Biotechnology, College of Engineering, Silla University) ;
  • Ha, Jong-Myung (Department of Bioscience and Biotechnology, College of Engineering, Silla University) ;
  • Lee, Jae-Hwa (Department of Bioscience and Biotechnology, College of Engineering, Silla University)
  • 이성목 (신라대학교 공과대학 생명공학과) ;
  • 유병조 (삼성종합기술원) ;
  • 김영민 (신라대학교 공과대학 생명공학과) ;
  • 최수정 (신라대학교 공과대학 생명공학과) ;
  • 하종명 (신라대학교 공과대학 생명공학과) ;
  • 이재화 (신라대학교 공과대학 생명공학과)
  • Received : 2009.02.12
  • Accepted : 2009.03.11
  • Published : 2009.06.10
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Abstract

Red-algae agar, consisting of D-galactose and 3, 6-anhydro-L-galactose, is usable for bio-ethanol production if hydrolyzed to monomer unit. The objective of this study is to produce bio-ethanol from agar using the heat and acid-treatment. Bio-ethanol was produced by Saccharomyces cerevisiae KCCM1129 strains using agar-pretreatment. The optimal condition for reducing sugar conversion by agar was found to be 15 min reaction at a HCl concentration of 0.1 N and $120^{\circ}C$. The optimum concentration for maximum cell growth was 0.1 N NaCl (17.88 g/L). Over 0.1 N NaCl, the cell growth decreased to 6.78~10.76 g/L. At 16% agar concentration, the ethanol production obtained by optimum pretreatment was found to be 10.16 g/L.

해조류 중에서도 홍조류의 agar는 D-galactose와 3,6-anhydro-L-galactose로 구성되어 있기 때문에 이를 분해하면 바이오 에탄올을 생산 할 수 있는 가능성이 높다. 본 연구에서는 열처리와 산 처리를 이용하여 agar를 당화하고 이를 통해 바이오 에탄올을 생산하고자 한다. 바이오 에탄올을 생산하기 위하여 전처리 된 agar에 Saccharomyces cerevisiae KCCM1129를 접종하여 발효하였다. Agar로부터 환원당 생성의 최적조건은 0.1 N HCl이었고, $120^{\circ}C$에서 15 min 반응하는 것으로 확인되었다. 발효균주 성장을 위한 최적 염 농도는 0.1 N NaCl로 17.88 g/L까지 성장하였으며, 0.1 N 이상의 농도에서 6.78~10.76 g/L로 성장이 감소했다. 그리고 agar 16% 농도에서 최적 전처리에 의한 에탄올 생산은 10.16 g/L이었다.

Keywords

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