Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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The Effector Functions of Mature T Lymphocytes Are Impaired in Transgenic Mice Expressing the SH2 Domain of TSAd/Lad

  • Choi, Youngbong (Department of Life Science, Ewha Womans University) ;
  • Park, Eunkyung (Department of Life Science, Ewha Womans University) ;
  • Ahn, Eunseon (Department of Life Science, Ewha Womans University) ;
  • Park, Inyoung (Department of Life Science, Ewha Womans University) ;
  • Yun, Yungdae (Department of Life Science, Ewha Womans University)
  • Received : 2009.04.21
  • Accepted : 2009.07.27
  • Published : 2009.09.30
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Abstract

TSAd/Lad is a T cell adaptor molecule involved in $p56^{lck}$-mediated T cell activation. To investigate the functions of TSAd in T cells, we generated transgenic (TG) mice expressing the SH2 domain of TSAd (TSAd-SH2) under the control of the $p56^{lck}$ proximal promoter. In T cells from TSAd-SH2 TG mice, T cell receptor (TCR)-mediated early signaling events, such as $Ca^{2+}$ flux and ERK activation, were normal; however, late activation events, such as IL-2 production and proliferation, were significantly reduced. Moreover, TCR-induced cell adhesion to extracellular matrix (ECM) proteins and migration through ECM proteins were defective in T cells from TSAd-SH2 TG mice. Furthermore, the contact hypersensitivity (CHS) reaction, an inflammatory response mainly mediated by T helper 1 (Th1) cells, was inhibited in TSAd-SH2 TG mice. Taken together, these results show that TSAd, particularly the SH2 domain of TSAd, is essential for the effector functions of T cells.

Keywords

Acknowledgement

Supported by : Center for Cell Signaling and Drug Discovery, Ewha Womans University

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