Journal of the Korean Wood Science and Technology

The Korean Society of Wood Science & Technology (한국목재공학회)
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Antifungal Activity of Extracts from Black Heartwood of Cryptomeria japonica against Dermatophytes

삼나무 흑심재 추출물의 피부사상균에 대한 항진균 활성

  • Lee, Su-Yeon (Dept. of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University) ;
  • Gwak, Ki-Seob (Dept. of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University) ;
  • Lee, Jun-Jae (Dept. of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University) ;
  • Yeo, Hwanmyeong (Dept. of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University) ;
  • Choi, In-Gyu (Dept. of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University)
  • 이수연 (서울대학교 농업생명과학대학 산림과학부) ;
  • 곽기섭 (서울대학교 농업생명과학대학 산림과학부) ;
  • 이전제 (서울대학교 농업생명과학대학 산림과학부) ;
  • 여환명 (서울대학교 농업생명과학대학 산림과학부) ;
  • 최인규 (서울대학교 농업생명과학대학 산림과학부)
  • Received : 2009.01.12
  • Accepted : 2009.02.16
  • Published : 2009.05.25
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Abstract

The aim of this study was to investigate the antifungal activitiy of extracts from different parts of Cryptomeria japonica against Trichophyton mentagrophytes, Trichophyton rubrum and Microsporum gypseum. Disc diffusion and agar dilution methods were used for determining the antifungal activity of extracts, and their major constituents were analyzed by GC/MS. Black heartwood extract among the extracts showed the highest antifungal activity against dermatophytes. TLC was used for fractionating the effective fraction from the black heartwood extract with n-hexane and chloroform as developing solvents, and then antifungal activity of each fraction was examined against dermatophytes. As a result, seventh fraction showed the highest antifungal activity among nine fractions. The major constituent of the seventh fraction determined by GC/MS was expected to be sesquiterpene, damascone with 2 more carbon atoms. The other constituents were also identified as elemol, eudesmol and hinokione.

본 연구는 삼나무 부위별 추출물들의 피부사상균 Trichophyton mentagrophytes, Trichophyton rubrum, Microsporum gypseum에 대한 항진균 활성을 평가하였다. 항진균 활성의 평가는 디스크확산법과 한천희석법을 사용하였고 활성 성분의 주성분을 확인하기 위해 GC/MS 분석을 실시하였다. 항진균 활성은 삼나무의 정상재와 흑심재 중 흑심재의 심재가 가장 높은 활성을 보였다. 활성 성분은 n-hexane과 chloroform를 전개용매로 이용하여 TLC를 실시하였다. TLC를 통해 분리된 활성 성분들에 대해 다시 항진균 활성을 측정한 결과 7번째 분획물이 가장 높은 활성을 나타내었다. 분획물에 대하여 GC/MS 분석을 실시한 결과 탄소개수 13개를 가지는 damascone에 탄소 2개가 더해진 sesquiterpene으로 예상되었다. 이밖에 elemol, eudesmol, hinokione 등도 검출되었다.

Keywords

Acknowledgement

Supported by : 산림청

References

  1. 곽기섭, 박미진, 정의배, 장제원, 최인규. 2006. 피부사상균에 대한 편백정유의 mono 및 sesqui-terpene 항 진균 활성 비교. 목재공학. 34(3): 46∼55
  2. 나승영, 오서전, 김홍준, 김종문, 주영승, 정승일. 2006. 삼나무(Cryptomeria japonica) 정유성분의 화학적 구성과 항균효과. 한방안이비인후피부과학회지. 19(3): 68∼74
  3. 박미진, 이수민, 곽기섭, 정의배, 장제원, 최인규. 2005. 피부사상균 Microsporum canis 및 Trichophyton mentagrophytes에 대한 편백정유의 항진균활성물질 탐색. 목재공학. 33(3): 72∼78
  4. 박장규, 이우재, 김경훈, 서기범. 1995. 충청지방의 표재성 피부사상균증의 임상 및 균학적 관찰. 대한피부과학회지. 33(1): 92∼99
  5. 유희준, 최연상, 백영곤, 손숙자. 1994. 서울지방 백선증의 원인균 분포(1985∼1991). 대한피부과학회지. 32(1): 24∼33
  6. 이기왕, 김홍직. 1984. 중복 감염된 표재성 진균증의 균학적 및 면역학적 연구. 대한피부과학회지. 22(4): 363∼368
  7. Arihara, S., A. Umeyama, S. Bando, S. Imoto, M. Ono, M. Tani, and K. Yoshikawa. 2004a. A New abietane and two dimeric abietane diterpenes from the black heartwood of Cryptomeria japonica. Chem. Pharm. Bull. 52(3): 354$\sim$358 https://doi.org/10.1248/cpb.52.354
  8. Arihara, S., A. Umeyama, S. Bando, S. Imoto, M. Ono, and K. Yoshikawa. 2004b. Three new sesquiterpenes from the black heartwood of Cryptomeria japonica. Chem. Pharm. Bull. 52(4): 463∼465 https://doi.org/10.1248/cpb.52.463
  9. Chang, S. T., P. F. Chen, S. Y. Wang, and H. H. Wu. 2001. Antimite activity of essential oils and their constituents from Taiwania cryptomerioides. J. Med. Entomol. 38: 455∼457 https://doi.org/10.1603/0022-2585-38.3.455
  10. Chen, X., C. S. Kim, T. Kashiwagi, S. Tebayashi, and M. Horiike. 2001. Antifeedant against Acusta despesta from the jaspanese cedar, Cryptomeria japonica. Biosci. Biotechnol. Biochem. 65(6): 1434 ∼1437 https://doi.org/10.1271/bbb.65.1434
  11. Cheng, S. S., H. Y. Lin, and S. T. Chang. 2005. Chemical composition and antifungal activity of essential oils from different tissues of japanese cedar (Cryptomeria japonica). J. Agric. Food Chem. 53: 614∼619 https://doi.org/10.1021/jf0484529
  12. Ishiguri, F., S. Maruyama, K. Takahashi, Z. Abe, S. Yokota, M. Andoh, and N. Yoshizawa. 2003. Extractives relating to heartwood color changes in sugi by a combination of smoke-heating and UV radiation exposure. J. Wood Sci. 49: 135∼139 https://doi.org/10.1007/s100860300021
  13. Kawachi, S., S. Meguro, and S. Inada. 1991. Cultivation of shiitake (Lentinus edodes) on wood-meal medium of Cryptomeria japonica. Inhibitory effect of ferruginol on mycelial growth (in Japanese). Mokuzai Gakkaishi 37: 971∼975
  14. Kofujita, H., Y. Fujino, T. Sasaki, M. Hasebe, M. Ota, and K. Suzuki. 2001. Antifungal activity of the bark of Cryptomeria japonica and its relevant components (in Japanese). Mokuzai Gakkaishi 47: 479∼486
  15. Kofujita, H., M. Ota, K. Takahashi, Y. Kawi, and Y. Hayashi. 2002. A diterpene quinone from the bark of Cryptomeria japonica. Phytochemistry 61: 895∼898 https://doi.org/10.1016/S0031-9422(02)00352-7
  16. Kubo, T. and S. Ataka. 1998. Blackening of sugi (Cryptomeria japonica D. Don) heartwood in relation to metal content and moisture content. J. Wood Sci. 44: 137∼141 https://doi.org/10.1007/BF00526259
  17. Male, O. 1990. The significance of mycology in medicine. In: Hawksworth, D.L., Ed. Frontier in mycology. CAB International, Wallingford, UK. pp. 131∼156
  18. Matsushita, Y., Y. Hwang, K. Sugamoto, and T. Matsui. 2006. Antimicrobial activity of heartwood components of sugi (Cryptomeria japonica) against several fungi and bacteria. J. Wood Sci. 52: 552∼556 https://doi.org/10.1007/s10086-005-0793-9
  19. Matsui, T., Y. Matsushita, K. Sugamoto, K. Ogawa, A. Kimiyama, and S. Muta. 2001. Mycelial growth inhibition of shiitake (Lentinula edodes) by several terpenoids isolated from sugi (Cryptomeria japonica) wood (in Japanese). Mokuzai Gakkaishi 47: 58∼62
  20. Nakajima, K., T. Yoshimoto, and T. Fukuzumi. 1980. Substances inhibiting growth of shiitake mycelium in sugi wood (Cryptomeria japonica D. Don) (in Japanese). Mokuzai Gakkaishi 26: 698$\sim$702