Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Astaxanthin Inhibits $H_2O_2$-Mediated Apoptotic Cell Death in Mouse Neural Progenitor Cells via Modulation of P38 and MEK Signaling Pathways

  • Kim, Jeong-Hwan (Department of Biomaterial Control, Dong-Eui University) ;
  • Choi, Woo-Bong (Department of Biomaterial Control, Dong-Eui University) ;
  • Lee, Jong-Hwan (Department of Biomaterial Control, Dong-Eui University) ;
  • Jeon, Sung-Jong (Department of Biomaterial Control, Dong-Eui University) ;
  • Choi, Yung-Hyun (Department of Biomaterial Control, Dong-Eui University) ;
  • Kim, Byung-Woo (Department of Biomaterial Control, Dong-Eui University) ;
  • Chang, Hyo-Ihl (Department of Biotechnology, School of Life Sciences and Biotechnology, Korea University) ;
  • Nam, Soo-Wan (Department of Biomaterial Control, Dong-Eui University)
  • Published : 2009.11.30
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Abstract

In the present study, the neuroprotective effects of astaxanthin on $H_2O_2$-mediated apoptotic cell death, using cultured mouse neural progenitor cells (mNPCs), were investigated. To cause apoptotic cell death, mNPCs were pretreated with astaxanthin for 8 h and followed by treatment of 0.3 mM $H_2O_2$. Pretreatment of mNPCs with astaxanthin significantly inhibited $H_2O_2$-mediated apoptosis and induced cell growth in a dose-dependent manner. In Western blot analysis, astaxanthin-pretreated cells showed the activation of p-Akt, p-MEK, p-ERK, and Bcl-2, and the reduction of p-P38, p-SAPK/JNK, Bax, p-GSK3b, cytochrome c, caspase-3, and PARP. Because $H_2O_2$ triggers caspases activation, this study examined whether astaxanthin can inhibit caspases activation in $H_2O_2$-treated mNPCs. After $H_2O_2$ treatment, caspases activities were prominently increased, but astaxanthin pretreatment significantly inhibited $H_2O_2$-mediated caspases activation. Astaxanthin pretreatment also significantly recovered the ATP production ability of $H_2O_2$-treated cells. These findings indicate that astaxanthin inhibits $H_2O_2$-mediated apoptotic features in mNPCs. Inhibition assays with SB203580 ($10\;{\mu}M$, a specific inhibitor of p38) and PD98059 ($10\;{\mu}M$, a specific inhibitor of MEK) clearly showed that astaxanthin can inhibit $H_2O_2$-mediated apoptotic death via modulation of p38 and MEK signaling pathways.

Keywords

References

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