Journal of the korean academy of Pediatric Dentistry (대한소아치과학회지)

Korean Academy of Pediatric Dentistry (대한소아치과학회)
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DETECTION SYSTEM OF STREPTOCOCCUS MUTANS IN SALIVA USING MONOCLONAL ANTIBODY

Monoclonal Antibody를 이용한 Streptococcus mutans 검출 방법의 임상적 적용에 관한 연구

  • Hong, Hi-Jung (Dankook University, School of Dentistry, Department of Pediatric Dentistry) ;
  • Kim, Jong-Soo (Dankook University, School of Dentistry, Department of Pediatric Dentistry) ;
  • Kim, Yong-Kee (Dankook University, School of Dentistry, Department of Pediatric Dentistry)
  • 홍희정 (단국대학교 치과대학 소아치과학교실) ;
  • 김종수 (단국대학교 치과대학 소아치과학교실) ;
  • 김용기 (단국대학교 치과대학 소아치과학교실)
  • Published : 2009.11.30
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Abstract

The purpose of this study was to evaluate the clinical effectiveness of new streptococcus detection system which used monoclonal antibody against Streptococcus mutans. 92 children aged between 2 and 8 were involved in this experiment and their saliva samples were collected for testing. Streptococcus mutans were measured by both monoclonal antibody-based detecting system (Saliva-$check^{TM}$ Mutans) and dip slide detecting system($Dentocult^{TM}$-SM). The results showed that Saliva-$check^{TM}$ Mutans levels had a significant correlation with dfs rate of subjects and the two test kits, Saliva-$check^{TM}$ Mutans and $Dentocult^{TM}$-SM were shown to have a good correlation although they were based on different mechanism.

Monoclonal antibody를 이용한 Saliva-$check^{TM}$ Mutans키트의 타액 Streptococcus mutans검출방법으로서의 활용도와 임상적 우식지수 및 기존의 세균배양방법과의 상관관계를 알아보기 위해 2008년 2월에서 5월 중 평촌키즈웰치과에 내원한 만 2세에서 만 8세 사이의 92명의 아동을 대상으로 Streptococcus mutans 검출검사를 실시하였으며 또한 우식에 영향을 미치는 다른 요인인 치면세균막 pH와 타액완충능력검사를 실시하여 다음과 같은 결론을 얻었다. 1. Saliva-$check^{TM}$ Mutans 검사결과 양성을 나타낸 아동은 27명으로 29.3%이었고, 음성을 나타낸 아동은 65명으로 70.65%이었다. 우식경험유치면률은 음성 아동 13.89%, 양성 아동 25.23%으로 나타났다. 2. Monoclonal antibody를 이용한 검사방법 인 Saliva-$check^{TM}$ Mutans와 기존의 세균 배양방법인 $Dentocult^{(R)}$-SM은 각각 상이한 검사방법을 이용함에도 불구하고 검사결과 높은 상관관계를 보였다(p<0.01). 3. Saliva-$check^{TM}$ Mutans검사와 치면세균막 pH검사와는 역상관관계를 나타내었으나(p<0.01),타액완충능 검사와는 상관관계가 나타나지 않았다(p>0.05). 이상의 결과로 보았을 때 Monoclonal antibody를 이용한 검사방법인 Saliva-$check^{TM}$ Mutans는 구강 내 Streptococcus mutans를 측정하는 방법으로 적당하며 또한 검사에 필요한 시간을 대폭 줄일 수 있고 사용방법도 매우 간편하게 개발되어 환자들에게 적용함에 있어 효과적이라고 사료되었다.

Keywords

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