Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Kaempferol Isolated from Nelumbo nucifera Stamens Negatively Regulates $Fc{\varepsilon}RI$ Expression in Human Basophilic KU812F Cells

  • Shim, Sun-Yup (Institute of Marine Life Science, Pukyong National University) ;
  • Choi, Jae-Sue (Division of Food Science and Biotechnology, Pukyong National University) ;
  • Byun, Dae-Seok (Division of Food Science and Biotechnology, Pukyong National University)
  • Published : 2009.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

Mast cells and basophils perform important functions as pivotal effector cells in IgE-mediated allergic reactions. KU812F cells, a human basophilic cell line isolated originally from chronic myelocytic leukemia, express a high affinity receptor of IgE, $Fc{\varepsilon}RI$. Kaempferol was extracted and isolated from a methanolic extract of flavonoid-rich Nelumbo nucifera stamens. In the present study, the inhibitory effects of kaempferol on $Fc{\varepsilon}RI$ expression in human basophilic KU812F cells was examined. Flow cytometric analysis revealed that $Fc{\varepsilon}RI$ expression on the cell surface was suppressed in a concentration-dependent manner when the cells were cultured with kaempferol. Moreover, RT-PCR analysis showed that the mRNA levels for $Fc{\varepsilon}RI$ ${\alpha}-\;and\;{\gamma}$-chains were reduced as the result of kaempferol treatment in a concentration-dependent manner. Kaempferol showed its suppressive effects on intracellular $Ca^{2+}$ concentration and histamine release from anti-$Fc{\varepsilon}RI$ ${\alpha}$-chain antibody-stimulated cells in a concentration-dependent manner. These observations indicate that kaempferol may exert antiallergic effect via down regulation of $Fc{\varepsilon}RI$ expression and degranulation.

Keywords

References

  1. Amoui, M., L. Drrova, P. Tolar, and P. Draber. 1997. Direct interaction of Syk and Lyn protein tyrosine kinases in rat basophilic leukemia cells activated via type Fc epsilon receptors. Eur. J. Immunol. 27: 321-328 https://doi.org/10.1002/eji.1830270146
  2. Blank, U., C. Ra, L. Miller, K. White, H. Metzer, and J. P. Kinet. 1989. Complete structure and expression in transfected cells of high affinity IgE receptor. Nature 337: 187-189 https://doi.org/10.1038/337187a0
  3. Cho, E. J., T. Yokozawa, D. Y. Rhyu, S. C. Kim, N. Shibahara, and J. C. Park. 2003. Study on the inhibitory effects of Korean medicinal plants and their main compounds on the 1,1- diphenyl-2-picrylhydrazyl radical. Phytomedicine 14: 544-551
  4. Drombrowicz, D., V. Flamand, K. K. Brigman, B. H. Koller, and J. P. Kinet. 1993. Abolition of anaphylaxis by targeted disruption of the high affinity immunoglobulin E receptor $\varepsilon$ chain gene. Cell 75: 969-976 https://doi.org/10.1016/0092-8674(93)90540-7
  5. Eiseman, E. and J. B. Bolen. 1992. Engagement of the highaffinity IgE receptor activates src protein-related tyrosine kinases. Nature 355: 78-80 https://doi.org/10.1038/355078a0
  6. Fujimura, Y., H. Tachibana, and K. Yamada. 2001. A tea catechin suppresses the expression of the high-affinity IgE receptor Fc$\varepsilon$RI in human basophilic KU812 cells. J. Agric. Food Chem. 49: 2527-2531 https://doi.org/10.1021/jf001392w
  7. Fujimura, Y., H. Tachibana, M. Maeda-Yamamoto, M. T. Miyase, M. Sano, and K. Yamada. 2002. Antiallergic tea catechin, (-)-epigallocatechin-3-O-(3-O-methyl)-gallate, suppresses Fc$\varepsilon$RI expression in human basophilic KU812 cells. J. Agric. Food Chem. 50: 5729-5734 https://doi.org/10.1021/jf025680z
  8. Hakimi, J. C., J. A. Seals, L. Kondas, W. Pettine, W. Danko, and J. Kochan. 1990. The $\alpha$ subunit of the human IgE receptor (Fc$\varepsilon$RI) is sufficient for high affinity IgE binding. J. Biol. Chem. 265: 22079-22081
  9. Hata, D., Y. Kawakami, N. Inagaki, C. S. Lantz, T. Kitamura, W. N. Khan, et al. 1998. Involement of Bruton's tyrosine kinase in Fc$\varepsilon$RI-dependent mast cell degranulation and cytokine production. J. Exp. Med. 187: 1235-1247 https://doi.org/10.1084/jem.187.8.1235
  10. Huxley, R. R. and H. A. Neil. 2003. The relation between dietary flavonol intake and coronary heart disease mortality: A meta-analysis of propoective cohort diseases. Eur. J. Clin. Nutr. 57: 904-908 https://doi.org/10.1038/sj.ejcn.1601624
  11. Jung, H. A., J. E. Kim, H. Y. Chung, and J. S. Choi. 2003.Antioxidant principles of Nelumbo nucifera stamens. Arch.Pharm. Res. 26: 279-285 https://doi.org/10.1007/BF02976956
  12. Kim, H. K., H. R. Park, J. S. Lee, T. S. Chung, and H. Y. Chung. 2007. Down-regulation of iNOS and TNF-alpha expression by kaempferol via NF-kappaB inactivation in aged rat gingival tissues. Biogerontology 8: 399-408 https://doi.org/10.1007/s10522-007-9083-9
  13. Kinet, J. P. 1999. The high affinity IgE receptor (FcεRI): From physiology to pathology. Annu. Rev. Immunol. 17: 931-972 https://doi.org/10.1146/annurev.immunol.17.1.931
  14. Kinet, J. P., U. Blank, A. Brini, M. H. Jouvin, H. Kuster, O. Mejan, and C. Ra. 1991. The high affinity receptor for immunoglobulin E: A target for therapy of allergic diseases. Int. Arch. Allergy Appl. Immunol. 94: 51-55 https://doi.org/10.1159/000235323
  15. Kishi, K. 1985. A new leukemia cell line with Philadelphia chromosome characterized as basophile precursors. Leuk. Res. 9: 381-390 https://doi.org/10.1016/0145-2126(85)90060-8
  16. La Cour, B., P. Molgaard, and Z. Yi. 1995. Traditional Chinese medicine in treatment of hyperlipidaemia. J. Ethnopharmacol. 46: 125-129 https://doi.org/10.1016/0378-8741(95)01234-5
  17. Lim, S. S., Y. J. Jung, S. K. Hyun, Y. S. Lee, and J. S. Choi. 2006. Rat lens aldose reductase inhibitory constituents of Nelumbo nucifera stamens. Phytother. Res. 20: 825-830 https://doi.org/10.1002/ptr.1847
  18. Metzer, H. 1991. The high affinity receptor for IgE on mast cells. Clin. Exp. Immunol. 4: 269-279
  19. Middleton, E. J. 1998. Effect of plant flavonoids on immune and inflammatory cell function. Adv. Exp. Med. Biol. 439: 175-182
  20. Nadler, M. J., S. A. Matthews, H. Turner, and J. P. Kinet. 2000. Signal transduction by the high-affinity immunoglobulin E receptor Fc$\varepsilon$RI coupling form to function. Adv. Immunol. 76: 325-355
  21. Nishiyama, C., M. Hasegawa, M. Nishiyama, K. Takahashi, Y. Akizawa, T. Yokota, K. Okumura, H. Ogawa, and C. Ra. 2002. Regulation of human Fc$\varepsilon$RI $\alpha$-chain gene expression by multiple transcription factors. J. Immunol. 168: 4546-4552 https://doi.org/10.4049/jimmunol.168.9.4546
  22. Nishiyama, C. 2006. Molecular mechanism of allergy-related gene regulation and hematopoietic cell development by transcription factors. Biosci. Biotechnol. Biochem. 70: 1-9 https://doi.org/10.1271/bbb.70.1
  23. Nishiyama, C., T. Ito, M. Nishiyama, S. Masaki, K. Maeda, N. Nakano, et al. 2005. GATA-1 is required for expression of Fc{epsilon} RI on mast cells: Analysis of mast cells derived from GATA-1 knockdown mouse bone marrow. Int. Immunol. 17: 847-856 https://doi.org/10.1093/intimm/dxh278
  24. Nishiyama, C., T. Yokota, K. Okumura, and C. Ra. 1999. The transcription factors Elf-1 and GATA-1 to cell-specific enhancer elements of human high affinity IgE receptor $\alpha$-chain gene. J. Immunol. 163: 623 630
  25. Schwartz, L. B., R. A. Lewis, D. Selden, and K. F. Austen. 1981. Acid hydrolases and tryptase from secretory granules of dispersed human lung mast cells. J. Immunol. 126: 1290-1294
  26. Siraganian, R. P., J. Zhang, K. Suzuki, and K. Sada. 2002. Protein tyrosine kinase Syk in mast cell signaling. Mol. Immunol. 38: 1229-1233 https://doi.org/10.1016/S0161-5890(02)00068-8
  27. Shore, P. A., A. Burkhalter, V. H. Cohn, and H. C. Victor. 1959. A method for the fluorometric assay of histamine in tissues. J. Pharmacol. Exp. Ther. 127: 182-186
  28. Suzuki, Y., T. Yoshimura, T. Matsui, T. Inoue, O. Niide, S. Nunomura, and C. Ra. 2003. Fc(epsilon)RI signaling of mast cells activates intracellular production of hydrogen peroxide: Role in the regulation of calcium. J. Immunol. 171: 6119-6127
  29. Takahashi, K., C. Nishiyama, and C. Ra. 1999. Transcriptional factors Elf-1 and GATA-1 bind to cell-specific enhancer elements of human-affinity IgE receptor alpha-chain gene. J. Immunol. 163: 623-630
  30. Turner, H. and J. P. Kinet. 1999. Signalling through the highaffinity IgE receptor Fc epsilonRI. Nature 25: 24-30
  31. Van Bergen, P. E., P. G. Hatcher, J. J. Boon, M. E. Collinson, and J. W. De Leeuw. 1997. Macromolecular composition of the propagule wall of Nelumbo nucifera. Phytochemistry 45: 601- 617 https://doi.org/10.1016/S0031-9422(96)00880-1
  32. Yono, S., H. Tachibana, and K. Yamada. 2005. Flavones suppress the expression of the high affinity IgE receptor Fc$\varepsilon$RI in human basophilic KU812F cells. J. Agric. Food Chem. 53: 1812-1817 https://doi.org/10.1021/jf047929d