Journal of Pharmaceutical Investigation

The Korean Society of Pharmaceutical Sciences and Technology (한국약제학회)
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Preparation and Cellular Uptake of Hydrophobic Quantum Dots Encapsulated in Poly-L-Lactic Acid Film

소수성 양자점을 함유한 Poly-L-Lactic Acid film의 제조 및 세포흡수 연구

  • Lee, Ji-Sook (Center for Chemoinformatics Research, Korea Institute of Science and Technology) ;
  • Woo, Kyoung-Ja (Nano-Materials Research Center, Korea Institute of Science and Technology) ;
  • Chung, He-Sson (Center for Chemoinformatics Research, Korea Institute of Science and Technology)
  • 이지숙 (한국과학기술연구원 생체과학연구본부) ;
  • 우경자 (한국과학기술연구원 나노과학연구본부) ;
  • 정혜선 (한국과학기술연구원 생체과학연구본부)
  • Published : 2009.02.20
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Abstract

To overcome the stability problem of hydrophilic quantum dot (Q-dot), cellular uptake of hydrophobic instead of hydrophilic Q-dot was studied in the hope to find a simple method to use Q-dot as a cellular imaging probe. Hydrophobic Q-dot and poly-L-lactic acid (PLLA) were co-dissolved in chloroform to prepare stable films. Due to the cellular compatibility of PLLA, adherent cells were cultured on the film to observe the degree of Q-dot uptake and cytotoxicity of the prepared films. The results show that Q-dots were absorbed into NIH3T3 and EMT6 cells. Cellular uptake was also observed when hydrophobic Q-dots were coated directly on a glass plate. PLLA/Q-dot film and Q-dot coated on glass plate did not show major cytotoxicity. In vivo tumor model was also used to show the uptake of Q-dot from the PLLA/Q-dot film to the tumor site.

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References

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