Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Processing and Biological Activity of Gelatin Hydrolysate from Branchiostegus japonicus Scales

옥돔(Branchiostegus japonicus) 비늘 유래 젤라틴의 가수분해 및 가수분해물의 기능성

  • Ahn, Yong-Seok (Department of Food Bioengineering, Jeju National University) ;
  • Lee, Won-Woo (Department of Marine Life Science, Jeju National University) ;
  • Lee, Seung-Hong (Department of Marine Life Science, Jeju National University) ;
  • Ahn, Gin-Nae (Department of Marine Life Science, Jeju National University) ;
  • Ko, Chang-Ik (Department of Marine Life Science, Jeju National University) ;
  • Oh, Chang-Kyung (Department of Tourism Hotel Culinary Art, Jeju College of Technology) ;
  • Oh, Myung-Cheol (Department of Tourism Hotel Culinary Art, Jeju College of Technology) ;
  • Kim, Dong-Woo (Central Research Center, Natural F&P Co., Ltd.) ;
  • Jeon, You-Jin (Department of Marine Life Science, Jeju National University) ;
  • Kim, Soo-Hyun (Department of Food Bioengineering, Jeju National University)
  • 안용석 (제주대학교 식품생명공학과) ;
  • 이원우 (제주대학교 해양생명과학과) ;
  • 이승홍 (제주대학교 해양생명과학과) ;
  • 안긴내 (제주대학교 해양생명과학과) ;
  • 고창익 (제주대학교 해양생명과학과) ;
  • 오창경 (제주산업정보대학 관광호텔조리과) ;
  • 오명철 (제주산업정보대학 관광호텔조리과) ;
  • 김동우 ((주)네추럴 F&P 중앙연구소) ;
  • 전유진 (제주대학교 해양생명과학과) ;
  • 김수현 (제주대학교 식품생명공학과)
  • Published : 2009.10.31
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Abstract

The potential utility of fish scales to the functional food industry has been investigated due to its antioxidant and antihypertensive characteristics. In this study, we report on the reactive oxygen species (ROS) scavenging and angiotensin I converting enzyme (ACE) inhibitory activities of gelatin hydrolysates processed from Branchiostegus japonicus scales, which are also high in protein content (about 46.1%). We prepared the enzymatic gelatin hydrolysates with four proteases (${\alpha}$-chymotrypsin, Alcalase, Neutrase and trypsin) from B. japonicus scale gelatin, which was prepared according to different reaction times, substrate/enzyme ratios and substrate concentrations. The enzymatic hydrolytic degrees of the gelatin increased time-dependently up to 6 hrs, while the Alcalase gelatin hydrolysates showed the highest hydrolysis degrees compared to the others. Furthermore, gelatin hydrolysates of Neutrase and ${\alpha}$-chymotrypsin showed the highest DPPH radical and $H_2O_2$ scavenging activities ($IC_{50}$ value; 9.18 mg/mL and 9.74 mg/mL), respectively. However, the activities were not significant (P<0.05). We also observed that the four gelatin hydrolysates significantly increased ACE inhibitory activities from approximately 20% to 60% (P<0.05), Among them, the Alcalase gelatin hydrolysates showed the higher ACE inhibitory activity ($IC_{50}$ value; 0.73 mg/mL) compared to the others. These results suggest that the enzymatic gelatin hydrolysates prepared from B. japonicus scales may possess a potentially useful function as an ACE inhibitory agent. As such, the utility of B. japonicus scales should be given due consideration for application in the functional food industry.

Keywords

References

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