한국동물위생학회지 (Korean Journal of Veterinary Service)

  • 제32권3호
  • /
  • Pages.227-239
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  • 2009
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  • 3022-7372(pISSN)
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  • 2287-7630(eISSN)
한국동물위생학회 (The Korean Society of Veterinary Service)
권호 표지

소와 돼지유래 살모넬라속균의 약계내성유전자의 특성에 관한 연구

Investigation on antimicrobial resistance genes of Salmonella spp. isolated from pigs and cattle

  • 이우원 (부산광역시 보건환경연구원 축산물위생검사소) ;
  • 정병열 (국립수의과학검역원) ;
  • 이강록 (부산광역시 보건환경연구원 축산물위생검사소) ;
  • 이동수 (부산광역시 보건환경연구원 축산물위생검사소) ;
  • 김용환 (경상대학교 수의과대학)
  • Lee, Woo-Won (Veterinary Service Laboratory, Busan Metropolitan City Institute of Health and Environment) ;
  • Jung, Byeong-Yeal (National Veterinary Research & Quarantine Service) ;
  • Lee, Gang-Rok (Veterinary Service Laboratory, Busan Metropolitan City Institute of Health and Environment) ;
  • Lee, Dong-Soo (Veterinary Service Laboratory, Busan Metropolitan City Institute of Health and Environment) ;
  • Kim, Yong-Hwan (College of Veterinary Medicine, Gyeongsang National University)
  • 발행 : 2009.09.30
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초록

At the present study, it was aimed to detect virulence genes and antimicrobial resistance genes among 102 strains of 12 Salmonella serotypes isolated from pigs and cattle. In polymerase chain reaction (PCR), invA was detected from all strains of Salmonella spp., spvC was detected from Salmonella enterica serotype Enteritidis (S. Enteritidis) (100%), S. Bradenburg (75%), and S. Typhimurium (20.4%). Drug resistance related genes of 12 types were detected from all strains. TEM ($bla_{TEM}$) gene was detected from 51 (92.7%) of 55 $\beta$-lactams (54 ampicillin or 1 amoxicillin) resistance strains. 55 (100%) of 55 chloramphenicol resistance strains, 3 (100%) of 3 gentamicin resistance strains and 5 (100%) of 5 kanamycin resistance strains did contain cml, aadB, and aphA1-Iab, respectively. strB (89.9%), strA (88.4%), aadA2 (84.1%) and aadA1 (72.5%) were detected from 69 streptomycin resistance strains. sulII and dhfrXII were detected from 49 (100%) of 49 sulfamethoxazole/trimethoprim resistance strains, but sulI was not detected. tetA (97.9%) and tetB (21.6%) were detected from 97 tetracycline resistance strains. int gene was detected from 58 (56.9%) of 102 strains. 54 S. Typhimurium of 102 Salmonella spp. were attempted to detect drug resistance genes. TEM was detected from 44 (95.7%) of 46 $\beta$-lactams (45 ampicillin or 1 amoxicillin) resistance strains. cmlA was detected from 51 (100%) of 51 chloramphenicol resistance strains. aadA2 (100%), strA (100%), strB (100%), and aadA1 (79.6%) were detected from 54 streptomycin resistance strains. sulII (100%) and dhfrXII (100%) were detected from 49 sulfamethoxazole/trimethoprim resistance strains. tetA was detected from 54 (100%) of 54 tetracycline resistance strains. int gene was detected from 54 (100%) of 54 strains. The major drug resistance pattern and resistance gene profile were ampicillin, chloramphenicol, streptomycin, sulfamethoxazole/trimethoprim and tetracycline (ACSSuT) and TEM, cmlA, aadA1, aadA2, strA, strB, sulII, dhfrXII, tetA and int, respectively.

키워드

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