Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Molecular Characterization of the Perilla frutescens Limonene Gene (PFLS) by Agroinfiltration into Nicotiana benthamiana

들깨 Limonene 유전자의 담배식물(Nicotiana benthamiana)내 Agroinfiltration에 의한 분자적 특성

  • Seong, Eun-Soo (Bioherb Research Institute, Kangwon National University) ;
  • Seo, Eun-Won (Bioherb Research Institute, Kangwon National University) ;
  • Kim, Hyoung-Seok (Department of Natural Resources and Environment Science, University of Illinois) ;
  • Heo, Kweon (Division of Bio-resources Technology, College of Agriculture and Life Science, Kangwon National University) ;
  • Lee, Ju-Kyung (Division of Bio-resources Technology, College of Agriculture and Life Science, Kangwon National University) ;
  • Chung, Ill-Min (Department of Applied Life Science, Konkuk University) ;
  • Ghimire, Bimal Kumar (Department of Applied Life Science, Konkuk University) ;
  • Kim, Myong-Jo (Bioherb Research Institute, Kangwon National University) ;
  • Lim, Jung-Dae (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Yu, Chang-Yeon (Bioherb Research Institute, Kangwon National University)
  • 성은수 (강원대학교 한방바이오연구소) ;
  • 서은원 (강원대학교 한방바이오연구소) ;
  • 김형석 (미국 일리노이대학 자연자원환경과학과) ;
  • 허권 (강원대학교 식물자원응용공학부) ;
  • 이주경 (강원대학교 식물자원응용공학부) ;
  • 정일민 (건국대학교 응용생명과학과) ;
  • 비말 (건국대학교 응용생명과학과) ;
  • 김명조 (강원대학교 한방바이오연구소) ;
  • 임정대 (강원대학교 삼척캠퍼스 생약자원개발학과) ;
  • 유창연 (강원대학교 한방바이오연구소)
  • Published : 2009.02.28
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Abstract

The full-length cDNA encoding Perilla frutescens limonene synthase (PFLS) (603 amino acids, GenBank accession no. D49368) was cloned. To elucidate the role of PFLS in gene regulation, we transiently transformed full-length PFLS into tobacco plants. PFLS mRNA was first detected in the intact leaves of the plants at 6 h, and the LS transcript level increased after 12 h in leaves treated with oxidative stress-related chemicals. The transient overexpression of PFLS resulted in increased transcription of NbPR1 and NbSIP in Nicotiana benthamiana leaves. Thus, our result confirmed that the infiltration of PFLS gene act as a transcriptional regulator of NbPR1 or NbSIP genes in the tobacco.

Keywords

References

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