Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
권호 표지
DOI QR코드

DOI QR Code

THE EFFECT OF PRIMING ETCHED DENTIN WITH SOLVENT ON THE MICROTENSILE BOND STRENGTH OF HYDROPHOBIC DENTIN ADHESIVE

산 부식된 상아질에 대한 용매를 이용한 프라이밍이 소수성 상아질 접착제의 미세인장접착강도에 미치는 영향

  • Park, Eun-Sook (Department of Conservative Dentistry, School of Dentistry, Seoul National University) ;
  • Bae, Ji-Hyun (Department of Conservative Dentistry, Seoul National University Bundang Hospital) ;
  • Kim, Jong-Soon (Department of Conservative Dentistry, School of Dentistry, Seoul National University) ;
  • Kim, Jae-Hoon (Department of Conservative Dentistry, School of Dentistry, Seoul National University) ;
  • Lee, In-Bog (Department of Conservative Dentistry, School of Dentistry, Seoul National University) ;
  • Kim, Chang-Keun (Department of Chemical Engineering, Choongang University) ;
  • Son, Ho-Hyun (Department of Conservative Dentistry, School of Dentistry, Seoul National University) ;
  • Cho, Byeong-Hoon (Department of Conservative Dentistry, School of Dentistry, Seoul National University)
  • 박은숙 (서울대학교 치과대학 치과보존학교실) ;
  • 배지현 (서울대학교 분당병원 치과 보존과) ;
  • 김종순 (서울대학교 치과대학 치과보존학교실) ;
  • 김재훈 (서울대학교 치과대학 치과보존학교실) ;
  • 이인복 (서울대학교 치과대학 치과보존학교실) ;
  • 김창근 (중앙대학교 화학신소재공학부) ;
  • 손호현 (서울대학교 치과대학 치과보존학교실) ;
  • 조병훈 (서울대학교 치과대학 치과보존학교실)
  • Published : 2009.01.30
Download PDF
⟨ Previous Next ⟩

Abstract

Deterioration of long-term dentin adhesion durability is thought to occur by hydrolytic degradation within hydrophilic domains of the adhesive and hybrid layers. This study investigated the hypothesis that priming the collagen network with an organic solvent displace water without collapse and thereby obtain good bond strength with an adhesive made of hydrophobic monomers and organic solvents. Three experimental adhesives were prepared by dissolving two hydrophobic monomers, bisphenol-A-glycidylmethacrylate (Bis-GMA) and triethyleneglycol dimethacrylate (TEGDMA), into acetone, ethanol or methanol. After an etching and rinsing procedure, the adhesives were applied onto either wet dentin surfaces (wet bonding) or dentin surfaces primed with the same solvent (solvent-primed bonding). Microtensile bond strength (MTBS) was measured at 48 hrs, 1 month and after 10,000 times of thermocycles. The bonded interfaces were evaluated using a scanning electron microscope (SEM). Regardless of bonding protocols, well-developed hybrid layers were observed at the bonded interface in most specimens. The highest mean MTBS was observed in the adhesive containing ethanol at 48 hrs. With solvent-primed bonding, increased MTBS tendencies were seen with thermo cycling in the adhesives containing ethanol or methanol. However, in the case of wet bonding, no increase in MTBS was observed with aging.

장기적인 상아질 접착의 내구성 악화는 접착층과 혼성충의 친수성 부위에서의 가수분해에 의해 일어나는 것으로 보고 되고 있다. 본 연구의 가설은 콜라겐 망상체를 유기용매로 프라이밍하면 콜라겐 조직을 붕괴시키지 않고 수분을 밀어내고 소수성 단량체와 유기용매로 이루어진 접착제가 침투하여 접착강도를 얻을 수 있다는 것이다. 두 소수성 단량체인 Bisphenol-A-glycidylmethacrylate (Bis-GMA)와 triethyleneglycol dimethacrylate (TEGDMA)를 아세톤, 에탄올 또는 메탄올에 용해시켜 세 가지의 실험용 접착제를 준비하였다. 산 부식과 수세과정 후에, 접착제를 습윤 상아질 표면(습윤 접착)이나 동일한 용매로 프라이밍 된 상아질 표면(용매 프라이밍 접착)에 적용하였다. 48시간 후와 1개월 후, 및 10,000회의 열순환 후에 미세인장결합강도를 측정하였다. 접착계면은 주사전자현미경을 이용하여 관찰하였다. 접착 방법에 무관하게 대부분의 시편의 접착계면에서 잘 발달된 혼성층을 관찰할 수 있었고, 가장 높은 평균 미세인장결합강도는 에탄올을 포함하는 접착제의 48시간 후 시편에서 관찰되었다. 용매를 이용하여 프라이밍하는 접착 방법에서는 에탄올이나 메탄올을 포함하는 접착제에서 열순환 후에 미세인장접착강도가 증가하는 경향을 보였다. 그러나, 습윤 접착의 경우에는 시효처리 후 미세인장결합강도의 증가가 관찰되지 않았다. 본 연구에서 에탄올을 이용한 상아질 프라이밍으로 우수한 접착력을 얻을 수 있었고, 열순환 후 접착력 이 더욱 증가하였다.

Keywords

References

  1. Nakabayashi N, Kojima K, Masuhara E. The promotion of adhesion by the infiltration of monomers into tooth substrates. J Biomed Mater Res 16:265-273, 1982 https://doi.org/10.1002/jbm.820160307
  2. Rosales-Leal JI, Osorio R, Holgado-Terriza JA, Cabrerizo-Vilchez MA, Toledano M. Dentin wetting by four adhesive systems. Dent Mater 17:526-532, 2001 https://doi.org/10.1016/S0109-5641(01)00014-8
  3. Eliades G, Vougiouklakis G, Palaghias G. Heterogeneous distribution of single-bottle adhesive monomers in the resin-dentin interdiffusion zone. Dent Mater 17:277-283, 2001 https://doi.org/10.1016/S0109-5641(00)00082-8
  4. Wang Y, Spencer P. Evaluation of the interface between one-bottle adhesive systems and dentin by Goldner's trichrome. Am J Dent 18:66-72, 2005
  5. Spencer P, Wang Y. Adhesive phase separation at the dentin interface under wet bonding conditions. J Biomed Mater Res 62:447-456, 2002 https://doi.org/10.1002/jbm.10364
  6. Spencer P, Wang Y, Lawrence K. Identification of collagen encapsulation at the dentin/adhesive interface. J Adhes Dent 6:91-95, 2004
  7. Tay FR, Pashley DH, Suh BI, Carvalho RM, Itthagarun A. Single-step adhesives are permeable membranes. J Dent 30:371-382, 2002 https://doi.org/10.1016/S0300-5712(02)00064-7
  8. Tay FR, Frankenberger R, Krejci I, Bouillaguet S, Pashley DH, Carvalho RM, Lai CNS. Single-bottle adhesives behave as permeable membranes after polymerization. I. In vivo evidence. J Dent 32:611-621, 2004 https://doi.org/10.1016/j.jdent.2004.04.006
  9. Armstrong SR, Vargas MA, Qian F, Laffoon JE. Microtensile bond strength of a total-etch 3-step, total-etch 2-step, self-etch 2-step, and a self-etch 1-step dentin bonding system through 15-month water storage. J Adhes Dent 5:47-56, 2003
  10. Tay FR, Hashimoto M, Pashley DH, Peters MC, Lai SCN, Yiu CKY, Cheong C. Aging affects two modes of nanoleakage expression in bonded dentin. J Dent Res 82:537-541, 2003 https://doi.org/10.1177/154405910308200710
  11. De Munck J, Van Meerbeek B, Yoshida Y, Inoue S, Vargas M, Suzuki K, Lambrechts P, Vanherle G. Fouryear water degradation of total-etch adhesives bonded to dentin. J Dent 82:136-140, 2003 https://doi.org/10.1177/154405910308200212
  12. Hashimoto M, Ohno H, Sano H, Kaga M, Oguchi H. In vitro degradation of resin-dentin bonds analyzed by microtensile bond test, scanning and transmission electron microscopy. Biomaterials 24:3795-3803, 2003 https://doi.org/10.1016/S0142-9612(03)00262-X
  13. Son SJ, Chang JG, Kang SH, Yoo HM, Cho BH, Son HH. The nanoleakage patterns of experimental hydrophobic adhesive after load cycling. J Kor Acad Cons Dent 33(1):9-19, 2008 https://doi.org/10.5395/JKACD.2008.33.1.009
  14. Fukushima T, Inoue Y, Miyazaki K, Itoh T. Effect of primers containing N-methylolacrylamide or N-methylolmethacrylamide on dentin bond durability of a resin composite after 5 years. J Dent 29:227-234, 2001 https://doi.org/10.1016/S0300-5712(01)00002-1
  15. Nakajima M, Okuda M, Ogata M, Pereira PN, Tagami J, Pashley DH. The durability of a fluoride-releasing resin adhesive system to dentin. Oper Dent 28:186-192, 2003
  16. Osorio R, Erhardt MC, Pimenta LA, Osorio E, Toledano M. EDTA treatment improves resin-dentin bonds' resistance to degradation. J Dent Res 5;84:736-740, 2005 https://doi.org/10.1177/154405910508400810
  17. Maciel KT, Carvalho RM, Ringle RD, Preson CD, Russell CM, Pashley DH. The effects of acetone, ethanol, HEMA, and air on the stiffness of human decalcified dentin matrix. J Dent Res 75:1851-1858, 1996 https://doi.org/10.1177/00220345960750110601
  18. Fukuda K, Nezu T, Terada Y. The effects of alcoholic compounds on the stability of type I collagen studied by differential scanning calorimetry. Dent Mater J 19:221-228, 2000 https://doi.org/10.4012/dmj.19.221
  19. Nakabayashi N, Watanabe A, Gendusa NJ. Dentin adhesion of 'modified'4-META/MMA-TBB resin: function of HEMA. Dent Mater 8:259-264, 1992 https://doi.org/10.1016/0109-5641(92)90096-U
  20. Perdigao J, Frankenberger R. Effect of solvent and rewetting time on dentin adhesion. Qunitessence Int 32:385-390, 2001
  21. Cho BH, Dickens SH. Effects of the acetone content of single solution dentin bonding agents on the adhesive layer thickness and the microtensile bond strength. Dent Mater 20:107-115, 2004 https://doi.org/10.1016/S0109-5641(03)00071-X
  22. Mohan B, Kandaswamy D. A confocal microscopic evaluation of resin-dentin interface using adhesive systems with three different solvents bonded to dry and moist dentin-An in vitro study. Quintessence Int 36:511-521, 2005
  23. Rosenblatt J, Devereux B, Wallace DG. Injectable collagen as a pH-sensitive hydrogel. Biomaterials 15:985-995, 1994 https://doi.org/10.1016/0142-9612(94)90079-5
  24. Carvalho RM, Yoshiyama M, Brewer PD, Pashley DH. Dimensional changes of demineralized human dentine during preparation for scanning electron microscopy. Arch Oral Biol 41:379-386, 1996 https://doi.org/10.1016/0003-9969(95)00130-1
  25. Asmussen E. Peutzfeldt A. Short- and Long-term bonding efficacy of a self-etching, one-step adhesive. J Adhes Dent 5:41-45, 2003
  26. Miguez PA, Pereira MP, Swift EJ Jr. One-year tensile bond strengths of two self-etching primers to bovine enamel. J Esthet Restor Dent 16:243-248, 2004 https://doi.org/10.1111/j.1708-8240.2004.tb00044.x
  27. Park JS, Kim JS, Kim MS, Son HH, Kwon HC, Cho BH. Aging effect on the microtensile bond strength of self-etching adhesives. J Kor Acad Cons Dent 31(6):415-426, 2006 https://doi.org/10.5395/JKACD.2006.31.6.415
  28. Takahashi A, Inoue S, Kawamoto C, Ominato R, Tanaka T, Sato Y, Pereira PN, Sano H. In vivo longterm durability of the bond to dentin using two adhesive systems. J Adhes Dent 4:151-159, 2002
  29. Nakajima M, Okuda M, Ogata M, Pereira PN, Tagami J, Pashley DH. The durability of a fluoride-releasing resin adhesive system to dentin. Oper Dent 28:186-192, 2003
  30. Armstrong SR, Vargas MA, Chung I, Pashley DH, Campbell JA, Laffoon JE, Qian F. Resin-dentin interfacial ultrastructure and microtensile dentin bond strength after five-year water storage. Oper Dent 29:705-712, 2004
  31. Frankenberger R, Strobel WO, Lohbauer U, Kramer N, Petschelt A. The effect of six years of water storage on resin composite bonding to human dentin. J Biomed Mater Res B Appl Biomater 69(1):25-32, 2004 https://doi.org/10.1002/jbm.b.20024
  32. Tarumi H, Imazato S, Ehara A, Kato S, Ebi N, Ebisu S. Post-irradiation polymerization of composites containing bis-GMA and TEGDMA. Dent Mater 15:238-242, 1999 https://doi.org/10.1016/S0109-5641(99)00040-8
  33. Kim YS, Park SH, Choi GW, Choi KK. Effect of intermediate resin hydrophilicity on bond strength of single step adhesive. J Kor Acad Cons Dent 32(5):445-458, 2007 https://doi.org/10.5395/JKACD.2007.32.5.445