Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Intracullular Functions of the mas2+ Gene in the Fission Yeast, Schizosaccharomyces pombe

분열형 효모에서의 mas2+ 유전자의 세포 내 기능

  • Sin, Sang-Min (Faculty of Biological Science and Research Institute for Basic Science, Wonkwang University) ;
  • Cha, Jae-Young (Faculty of Biological Science and Research Institute for Basic Science, Wonkwang University) ;
  • Ha, Se-Eun (Faculty of Biological Science and Research Institute for Basic Science, Wonkwang University) ;
  • Sim, Sun-Mi (Faculty of Biological Science and Research Institute for Basic Science, Wonkwang University) ;
  • Kim, Hyoung-Do (Faculty of Biological Science and Research Institute for Basic Science, Wonkwang University) ;
  • Lee, Jung-Sup (Department of Biological Science Research Center for Proteineous Materials, Chosun University) ;
  • Park, Jong-Kun (Faculty of Biological Science and Research Institute for Basic Science, Wonkwang University)
  • 신상민 (원광대학교 생명과학부, 기초자연과학연구소) ;
  • 차재영 (원광대학교 생명과학부, 기초자연과학연구소) ;
  • 하세은 (원광대학교 생명과학부, 기초자연과학연구소) ;
  • 심선미 (원광대학교 생명과학부, 기초자연과학연구소) ;
  • 김형도 (원광대학교 생명과학부, 기초자연과학연구소) ;
  • 이정섭 (조선대학교 BK21 단백질활성제어 인력양성 사업팀) ;
  • 박종군 (원광대학교 생명과학부, 기초자연과학연구소)
  • Published : 2009.01.30
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Abstract

The regulation of gene expression plays an important role in cell cycle controls. In this study, a novel $mas2^+$ (mitosis associated protein) gene, a homolog of human SMARCAD1 was isolated and characterized from a fission yeast Schizosaccharomyces pombe (S. pombe) using gene-specific polymerase chain reaction. The isolated gene contained a complete open reading frame capable of encoding 922 amino acid residues with a typical promoter, as judged by nucleotide sequence analysis. It was also found that an SNF2 domain is located, which is involved in the chromosome remodeling. The quantitative analysis of the $mas2^+$ transcript against $adh1^+$ showed that the expression level of $mas2^+$ is high before septum formation in S. pombe. When $mas2^+$ null mutant cells were grown at 27 and $35^{\circ}C$, the cytokinesis of $mas2^+$ null mutant was greatly delayed and a large number of multi-septate and mis-segregated cells were produced. In addition, the number of multi-septate cells significantly increased. When cells were cultured in YES rich medium to increase proliferation, the abnormal phenotypes $mas2^+$ null mutant dramatically increased. These phenotypes could be rescued by an over-expression of the mast gene. The Mas2 protein localized in the nuclei of S. pombe, as evidenced by Mas2-EGFP signals. These results suggest that the $mas2^+$ is homologous to human SMARCAD1 gene and involved in septum formation and chromosome remodeling control.

유전자 발현의 조절은 세포주기 조절에 중요한 역할을 한다. 본 연구에서 새로운 $mas2^+$ (${\underline{m}}itosis$ ${\underline{as}}sociated$ protein) 유전자는 인간의 SMARCAD1와 상동성을 갖고 분열형 효모인 Schizosaccharomyces pombe (S. pombe)에서 gene-specific PCR 방법에 의해서 분리하였다. 분리된 유전자는 SNF2 도메인이 위치해 있고, 이것은 염색체 재구성에 관련되어 있다. $adh1^+$을 이용한 $mas2^+$ 전사체의 발현량 분석은 $mas2^+$의 발현수준은 S. pombe에서 격막 형성 전에 가장 높았다. $mas2^+$ 완전돌연변이의 세포분열은 26와 $35^{\circ}C$에서 지연되는 현상이 보였고, 다수익 다중 격막이나 핵분열이 일어나지 않는 세포들을 발견하였다. 세포들을 완전배지인 YES에서 증식을 증가시키기 위해서 배양했을 때, 정상과 다른 형태의 표현형을 가진 $mas2^+$ 완전돌연변이 세포들이 증가했다. 이런 표현형들은 $mas2^+$ 유전자의 과발현에 의해서 감소하였다. Mas2 단백질은 S. pombe의 핵내에 위치하였다. 이런 결과들은 $mas2^+$가 인간의 SMARCAD1과 상동성을 갖고 있고, 염색체 재구성과 격막 형성 조절에 사용된다는 것을 나타낸다.

Keywords

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